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Application of oroxylum indicum as anti-oxidative stress injury agent

An anti-oxidative stress, wood butterfly technology, applied in the field of medicine, can solve problems such as human side effects

Inactive Publication Date: 2020-12-01
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that drugs such as levamlodipine and dexmedetomidine can improve oxidative stress damage of vascular endothelial cells, but long-term use of these drugs will cause certain side effects on the human body (Zhang Yuan, Hai Rong, Lu Xin. L-amlodipine Effect of clodipine on oxidative stress injury of vascular endothelial cells[J]. Chinese Journal of Clinical Pharmacology, 2016(2):159-162; Influence of oxidative stress injury[J]. Journal of Practical Medicine, 2013,29(009):1397-1399.)

Method used

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  • Application of oroxylum indicum as anti-oxidative stress injury agent
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  • Application of oroxylum indicum as anti-oxidative stress injury agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1H 2 o 2 Establishment of induced oxidative stress injury model of human umbilical vein endothelial cells

[0052] 1. Obtaining Human Umbilical Vein Endothelial Cells

[0053] Human umbilical vein endothelial cells (HUVEC) in the logarithmic phase were taken, digested with trypsin, centrifuged at 1000r / min for 5min, removed the supernatant, added complete culture medium and blown to a single cell state, and adjusted the cell density to 5× 10 7 / L.

[0054] 2. Establishment of cellular oxidative stress injury model

[0055] In a 96-well plate, add 100 μL HUVEC to each well and store at 37°C, 5% CO 2 After adherent culture for 24 hours under the culture conditions, the supernatant was sucked off, and 2mmol / L, 1mmol / L, 800μmol / L, 600μmol / L, 400μmol / L, 200μmol / L, 100μmol / L prepared with DMEM culture solution were added respectively. L of H 2 o 2 100 μL, after 2 hours, 4 hours, and 6 hours, remove the supernatant, add 90 μL DMEM culture solution and 10 μL MTT...

Embodiment 2

[0057] Embodiment 2 wooden butterfly pair H 2 o 2 Effects of cell viability and cell morphology on induced oxidative stress injury model of human umbilical vein endothelial cells

[0058] 1. Experimental grouping: divided into 6 groups, namely normal group, model group, and different doses of wood butterfly administration groups (wood butterfly doses were 0.2, 0.4, 0.8, 1.6 μg / mL).

[0059] The used wood butterfly is: dry and mature seeds of the wood butterfly are refluxed and extracted with 70% ethanol, and the obtained wood butterfly extract is purified and enriched by using AB-8 macroporous adsorption resin.

[0060] The preparation method of wood butterfly extract is as follows:

[0061] (1) 70% ethanol reflux extraction: After powdering the dried and mature seeds of the wood butterfly, weigh the powder, first add 70% ethanol 10 times the mass of the powder, and soak overnight. Reflux extraction in water bath at 80°C for 90 minutes, filter while it is hot, add 70% ethan...

Embodiment 3

[0066] Embodiment 3 wood butterfly pair H 2 o 2 Influence of SOD, CAT, MDA, LDH levels on the oxidative stress injury model of human umbilical vein endothelial cells

[0067] 1. Experimental grouping: the same as the experimental grouping in Step 1 of Example 2.

[0068] 2. Determination of SOD, CAT, MDA, and LDH levels: Take the HUVEC cells obtained in Step 1 of Example 1 and plant them in 100 mm culture dishes, 10 mL per dish, at 37 ° C, 5% CO 2 After 24 hours of adherent culture under the culture conditions, the supernatant was sucked off, and 10mL DMEM culture solution was added to the normal group and the model group, and 10mL of different concentrations of wood butterfly extracts prepared with DMEM culture solution were added to the wood butterfly administration group, and pre-incubated After 24 hours, remove the supernatant, and add 200 μmol / L H 2 o 2 10 mL, after 4 hours of action, the supernatant was collected to measure the release of LDH, and the cells were col...

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Abstract

The invention relates to application of oroxylum indicum as an in-vivo anti-oxidative stress injury agent in medicines, relates to new medical application of oroxylum indicum, and belongs to the technical field of medicines. In particular, the oroxylum indicum can be used to protect vascular endothelial cells from oxidative stress damage. Research of the invention shows that: the oroxylum indicumcan obviously improve the cell viability of H2O2-induced human umbilical vein endothelial cells and the viability of superoxide dismutase (SOD) and catalase (CAT), reduces malonaldehyde (MDA) and lactic dehydrogenase (LDH) release and cell apoptosis, enhances the expression of Nrf2, HO-1 and NQO-1 proteins, and has an obvious effect of protecting in-vivo cells from oxidative stress injury.

Description

technical field [0001] The invention belongs to the technical field of medicine, and the invention relates to the application of wood butterfly as an anti-oxidative stress damage agent in medicine, and relates to a new medical application of wood butterfly. Background technique [0002] Oxidative stress is the loss of cellular redox balance between the level of reactive oxygen species and endogenous antioxidant capacity, the relative lack of antioxidant capacity, the relative increase of intracellular reactive oxygen species, and the increase of some toxic reactive oxygen species caused by the attack of biological macromolecules. and tissue damage. Oxidative stress damage caused by free radicals and reactive oxygen species plays a very important role in the occurrence and development of many diseases. [0003] Among them, oxidative stress injury of vascular endothelial cells is closely related to many diseases. Endothelial cells are located on the inner surface of blood ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P39/06A61P9/14A61K131/00
CPCA61K36/185A61P39/06A61P9/14A61K2236/333A61K2236/55
Inventor 张蕾彭倩赵钟祥周玖瑶周园
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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