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Application of stem cell-derived microvesicles in preparation of preparation for repairing scars

A technology of microvesicles and stem cells, applied in the application field of preparations, can solve the problems of slow skin pigmentation changes, stem cell ethics, safety and difficulty in storage and transportation, and slow wound healing.

Pending Publication Date: 2020-11-06
AFFILIATED HOSPITAL OF JINING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, issues such as ethics, safety, difficulty in storage and transportation of stem cells limit their clinical application
[0003] The current treatment methods for scars only include: for more serious depressed scars, atrophic scars and hypertrophic scars, surgical excision is the main method, but any surgical method cannot completely remove the scars, but only improves or corrects them to the maximum extent. Harm caused by scars, and new scars should be formed after surgery; the most commonly used drugs for treating scars clinically are triamcinolone, triamcinolone acetonide, dexamethasone, etc., but long-term large-scale use can cause Cushing’s syndrome and certain symptoms. side effects; Freon and other low-temperature cold sources are used to treat freckles, senile plaques, etc., and directly contact the lesion. The lesion will be necrotic and fall off after 7 to 10 days, and the epidermis will heal. Disadvantages such as scars; the chemical agent carbolic acid is applied on the surface of the scarred skin. After the skin of the scars falls off, fresh and tender pink skin will appear to achieve the purpose of removing scars. However, this method of removing scars is not suitable for people of yellow race.

Method used

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  • Application of stem cell-derived microvesicles in preparation of preparation for repairing scars
  • Application of stem cell-derived microvesicles in preparation of preparation for repairing scars
  • Application of stem cell-derived microvesicles in preparation of preparation for repairing scars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Obtaining microvesicles derived from umbilical cord mesenchymal stem cells (hucMSCs)

[0036](1) Isolation and culture of umbilical cord MSC: according to the established HucMSC isolation and identification method, in vitro culture and expansion of MSC (Qiao Chun et al. Human mesenchymal stem cells isolated from the umbilical cord. Cell Biol Int. 2008 Jan; 32 (1): 8-15), select healthy human MSCs with strong proliferative ability and good condition ( figure 1 In A), the growth area covered 80%, cultured in serum-free medium for 48 hours to collect the culture supernatant, centrifuged at 2000g for 10 minutes to remove cell debris, then it was MSC-CM, and refrigerated at -70°C for later use.

[0037] (2) Separation and purification of microvesicles in the supernatant secreted by umbilical cord mesenchymal stem cells: the collected MSC-CMs were subjected to differential centrifugation to discard cell debris and organelles, and then transferred to a 15ml 100 000Da MWCO ultr...

Embodiment 2

[0042] Construction of scar rat model and repair effect of hucMSC-microvesicles on scar rats

[0043] (1) Construction of SD rat skin scar model: 8-week-old female SD rats weighing 200 g were anesthetized with 10% chloral hydrate, and 8% Na 2 S was treated with hair removal, and a circular full-thickness skin defect wound with a diameter of 2 cm was prepared with sterile surgical instruments. Scabs formed on the 2nd to 3rd day, and a skin scar model was formed around the 7th day;

[0044] (2) Intervention treatment of HucMSC-microvesicles: set up three experimental groups, PBS control group, high concentration group, low concentration group (high concentration: 4.5×10 11 / experimental animal; low concentration: 4.5×10 8 / experimental animal); three rats in each group, each rat was injected with 200 μl of phosphate buffered saline (PBS) in the subcutaneous tissue around the scar to dissolve high-concentration and low-concentration microvesicles for treatment, and PBS was use...

Embodiment 3

[0054] Repair effect of human scar microvesicles

[0055] (1) Basic information and repair plan: 31-year-old female, underwent the first cesarean section in November 2017, and the second cesarean section in February 2020, resulting in scar tissue after surgery;

[0056] Restoration plan: divided into 6 treatments, each time 4.5×10 11 Particles / volume 200 μl. The nano-microvesicles are stored in the freezer layer of the refrigerator, take out one before use, and let it dissolve naturally at room temperature. After cleaning the scars, take pictures of the scars, and then lie down and apply the completely melted nano-microvesicles evenly on the scars, gently pat and massage until completely absorbed, and treat once every 3 days, initially 6 times.

[0057] (2) Observation of repair effect (such as Figure 8 shown):

[0058] 1) Gross evaluation of scar

[0059] ① Including the degree of scar protrusion: the protruding part of the scar gradually becomes flat;

[0060] ②Scar c...

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Abstract

The invention provides application of stem cell-derived microvesicles in preparation of a preparation for repairing scars, and relates to the technical field of scar skin repair. In the invention, theextracellular vesicles derived from the mesenchymal stem cells obviously inhibit the expression of collagen III, the collagen III in the treatment group of the high-concentration microvesicles and the low-concentration microvesicles is obviously lower than that in the PBS group, and the expression of collagen I also presents a decreasing trend; both the high-concentration microvesicles and the low-concentration microvesicles can promote reasonable distribution of a skin structure, specifically, the structure of collagen is more reasonable, and accessory structures are remarkably increased; therefore, the stem cell-derived microvesicles have a scar repairing effect, the low-concentration microvesicles and the high-concentration microvesicles can promote the repairing effect of scar skin, the scar area of the repaired high-concentration microvesicles is smaller than the treatment of the low-concentration microvesicles, the treatment effect is remarkable, and the stem cell-derived microvesicles can be used for preparing preparations for repairing scars.

Description

technical field [0001] The invention belongs to the technical field of skin scar repair, and in particular relates to the application of stem cell-derived microvesicles in the preparation of scar repair preparations. Background technique [0002] Scar (scar) usually refers to the appearance, morphology and histopathological changes of normal skin tissue caused by various traumas, and it is an inevitable product in the process of human wound repair. When the scar grows beyond a certain limit, various complications will occur, such as the destruction of appearance and dysfunction of function, etc., which will bring huge physical pain and mental pain to the patient, especially the scar left after burns, scalds, and severe trauma. Collagen is the main component of the extracellular matrix involved in wound healing. In normal human skin, there are mainly collagen Ⅰ and collagen Ⅲ. Collagen Ⅰ has a large content and acts as a scaffold. The ratio of collagen Ⅲ is 10:1. In the proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P17/02A61K8/99A61Q19/00
CPCA61K35/28A61P17/02A61K8/99A61Q19/00A61K2800/91
Inventor 张斌甘立军班博别庆丽陈彦戎吴佩佩贾庆志谢娜李莹澳魏东
Owner AFFILIATED HOSPITAL OF JINING MEDICAL UNIV
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