Pretreatment method of hot processed food and detection method of acrylamide

A technology of acrylamide and detection method, applied in the field of analysis and detection, can solve the problems of low detection accuracy, large interference of impurities and the like

Active Publication Date: 2020-10-02
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the sample pretreatment provided by GB 5009.204-2014 or SN / T 2096-2008, the impurity interference of the sample is large, and the detection accuracy is not high

Method used

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  • Pretreatment method of hot processed food and detection method of acrylamide
  • Pretreatment method of hot processed food and detection method of acrylamide
  • Pretreatment method of hot processed food and detection method of acrylamide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] (1) Preparation of acrylamide series standard working solutions

[0122] (1.1) Prepare 1.0 mg / mL acrylamide standard stock solution

[0123] Accurately weigh 10 mg of acrylamide standard substance (accurate to 0.01 mg), dissolve it in methanol in a 10 mL brown volumetric flask, adjust the volume to the mark, and store in a sealed, dark place at -20°C.

[0124] (1.2) Prepare 10mg / L acrylamide standard stock solution

[0125] Accurately draw 0.25mL of 1.0mg / mL acrylamide standard stock solution into a 25mL brown volumetric flask, dilute to the mark with methanol, and store in a sealed seal at -20°C in the dark.

[0126] (1.3) Prepare 1.0mg / L acrylamide standard solution

[0127] Accurately draw 1.00 mL of 10 mg / L acrylamide standard stock solution into a 10 mL brown volumetric flask, and dilute to the mark with 0.1% formic acid aqueous solution. The 10 mg / L acrylamide standard stock solution is preferably ready-to-use.

[0128] (1.4) Prepare 0.5mg / mL 13 C 3 -Acrylamide...

Embodiment 2

[0144] (1) Sample pretreatment

[0145] (1.1) First crush the potato chips with a pulverizer, accurately weigh 0.5 g (accurate to 0.0001 g) of the crushed potato chips sample into a 25 mL graduated centrifuge tube, add 40 μL of 5.0 μg / L 13 C 3 -Acrylamide internal standard application solution and 10mL ultrapure water, ultrasonically extract for 10min, add 5mL of dichloromethane to degrease, vortex for 5min, centrifuge at 10000r / min for 3min, take out, and the supernatant is the extract;

[0146] (1.2) Put 5.0mL of the sample extraction solution into a GCB / PSA column for purification, wherein the flow rate of the sample extraction solution is 2mL / min, and collect the sample purification solution into a 15mL graduated centrifuge tube;

[0147] (1.3) Add 0.75g potassium bromide to 5mL sample purification solution and vortex to dissolve, add 0.3mL sulfuric acid solution with a concentration of 0.3mol / L, 0.5mL potassium bromate solution with a mass volume percent concentration of...

Embodiment 3

[0157] Detect according to the method of Example 2, the difference from Example 2 is: the concentration of acrylamide in the sample liquid to be tested is 100 μg / kg, and the test result is the average value of 6 experiments, and the test results are shown in Table 4 shown.

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Abstract

The invention relates to the technical field of analysis and detection, in particular to a pretreatment method of hot processed food and a detection method of acrylamide. The pretreatment method comprises the following steps: crushing hot processed food, adding 13C3-acrylamide internal standard application liquid and water, mixing, extracting, mixing an obtained extraction system with dichloromethane, degreasing, purifying an obtained sample extraction liquid by adopting a GCB / PSA column, adding potassium bromide, a sulfuric acid solution and a potassium bromate solution, mixing, and deriving.The invention provides the pretreatment method. After pure water extraction, dichloromethane degreasing, GCB / PSA column purification and potassium bromate and potassium bromide derivation are adoptedto convert acrylamide into 2, 3-dibromoacrylamide, the molecular weight is increased, the polarity is weakened, the chromatographic retention capacity is enhanced, the matrix interference phenomenonin LC-MS / MS detection is greatly reduced, and the detection accuracy is high.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a pretreatment method for heat-processed food and a detection method for acrylamide. Background technique [0002] Acrylamide is a recognized neurotoxin and quasi-carcinogen. Both animal experiments and in vitro cell experiments have proved that acrylamide can also cause changes in genetic material. Thermally processed foods such as coffee and potato chips produce relatively high levels of acrylamide during their baking and frying processes. The determination of acrylamide in food is mainly by gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry. For example, the current standard detection methods for acrylamide in food mainly include "GB 5009.204-2014 National Food Safety Standard-Determination of Acrylamide in Food" And "SN / T 2096-2008 Determination of Acrylamide in Food - Gas Chromatography-Mass Spectrometry Isotope Internal S...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N2030/067
Inventor 王军淋蔡增轩胡争艳张念华赵永信吴平谷
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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