Preparation method of silk fibroin tissue engineering scaffold based on near-field electrospinning technology

Abstract

The invention discloses a preparation method of a silk fibroin tissue engineering scaffold based on a near-field electrospinning technology. The preparation method comprises the steps that a regenerated silk fibroin aqueous solution is mixed with a polyoxyethylene aqueous solution, the ratio of the mixed materials is adjusted, the viscosity characteristic of the whole mixture is changed, and a silk fibroin / PEO solution system which can be used for near-field electrospinning is prepared; then the near-field electrospinning printing technology is adopted, printing is carried out according to a scaffold model of a cross-shaped net structure circulating in a periodic cycle, and finally, the preparation of the silk fibroin tissue engineering scaffold is realized through a post-treatment processsuch as chemical modifier soaking. The preparation method is simple, the production efficiency is high, the inherent characteristics of the raw materials are not affected, the porosity of the prepared tissue engineering scaffold is high and adjustable, the pore size is proper, the mechanical property is good, and the specific surface area is large; and the biocompatibility and the biological activity of the scaffold material are good, the extracellular matrix can be simulated well, and the scaffold material is suitable for cell adhesion, proliferation and differentiation.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a method for preparing a silk fibroin tissue engineering scaffold based on near-field electrospinning technology. Background technique [0002] Tissue engineering refers to planting isolated group cells and stem cells in a natural or synthetic cell scaffold that can be gradually degraded and absorbed by the human body after in vitro culture and expansion. This kind of biomaterial scaffold can provide cells with survival The three-dimensional space is conducive to the cells to obtain sufficient nutrients, perform gas exchange, and eliminate waste materials, so that the cells can grow on the prefabricated three-dimensional scaffold, and then the material that adheres to the cells is implanted into the tissue defect, and the biological material is gradually degraded. At the same time, the planted cells continue to proliferate, so as to achieve the purpose of repairing tis...

AI Technical Summary

Problems solved by technology

The solvent casting / particle leaching method is to pour the polymer solution containing the porogen into the mold, and remove the porogen after the solvent volatilizes. This method is simple, low in cost, and the size and shape of the formed pores can be controlled. Organic solvents reduce the activity of biomolecules, and if the scaffold is too thick, the porogen in the center will be difficult to remove completely

The freeze-drying method is to sublimate the ice crystals in the material to form an internal porous structure under low temperature and low pressure conditions. The scaffold formed by this method has high porosity, good internal connectivity of the pores, and is easy to control the shape of the scaffold through the mold, but Pores formed have small pore size and poor controllability

The rapid prototyping method (3D printing technology) is to use the CAD model to directly drive the 3D printer, and quickly prepare the tissue engineering scaffold of any shape by means of additive manufacturing. The pore size, porosity and pore shape of the tissue engineering scaffold obtained by the 3D printing technology Controllable, the shape of the scaffold is controllable, and the preparation time is short, but in this process, the porosity formed is low, and special equipment such as a 3D printer is required

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