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Application of anabaena and/or spirulina extract as biostimulant in agricultural production

A technology of Anabaena and Spirulina, which is applied in the field of agricultural production, can solve the problems of difficult to obtain fully water-soluble cell extracts, few processed and extract products, and destroy the active components of algae, and achieves easy control of product quality, The effect of rapid fertilizer effect and mature breeding technology

Inactive Publication Date: 2020-09-18
秦山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the above patent reports, the method of crushing microalgae cells and obtaining extracts either requires the use of chemical substances such as acids and alkalis, or requires high-temperature treatment, which partially destroys the active components of the algae; the method of using enzymes or bacteria as tools Biochemical methods involve complex processes and high costs; grinding or ultrasonic methods are difficult to obtain fully water-soluble cell extracts, which have a certain distance from the current requirements of water and fertilizer integration.
Most of the microalgae-based biostimulants on the market are direct products of live algae cells or microalgae harvests, and few processed and extracted products

Method used

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  • Application of anabaena and/or spirulina extract as biostimulant in agricultural production
  • Application of anabaena and/or spirulina extract as biostimulant in agricultural production
  • Application of anabaena and/or spirulina extract as biostimulant in agricultural production

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preparation example Construction

[0042] The extract of anabaena and / or spirulina provided by the present invention is prepared by the following steps: breaking the wall of collected anabaena and / or spirulina at 4-45°C, extracting with water, and extracting The obtained algae fluid containing soluble algae cell extract and cell fragment particles not larger than 400nm is spray-dried to obtain the extract of Anabaena and / or Spirulina.

[0043] The present invention firstly collects the algal bodies of Anabaena and / or Spirulina. In the present invention, there is no special limitation on the source of the algae of the Anabaena and / or Spirulina, and the algae that grows naturally or artificially can be used. When the above-mentioned microalgae are cultivated artificially, artificially cultured spirulina or anabaena need to use a medium containing nitrogen, phosphorus, potassium, calcium, magnesium, iron, sulfur, boron, manganese, zinc, copper and cobalt, while providing carbon dioxide , adjust the pH value of th...

Embodiment 1

[0056] Preparation method of spirulina platensis extract

[0057] (1) The source and harvest of algae cells: when the Spirulina platensis in the natural water body is in the vigorous growth stage, the water pump pumps the algae water and separates the algae cells through a centrifuge to obtain fresh Spirulina platensis algae mud.

[0058] (2) Cell wall breaking: adjust the water content of the harvested algae mud to 20%, pass through a high-pressure homogenizer, break the algae cells under the conditions of a temperature of 10°C and a pressure of 500bar, and obtain a compound containing cell contents and broken cell walls Extract. Using a microscope to visually inspect, under the 800X and 1600X lenses, the extract solution is homogeneous, without cells or cell debris, and the particle size is about 400 mesh nanoparticles.

[0059] (3) Extract drying: the algae cell extract treated by the homogenizer is spray-dried under the conditions of inlet temperature 165°C, outlet temper...

Embodiment 2

[0062] Preparation method of nitrogen-fixing anabaena extract

[0063] (1) The source and harvest of algae cells: During the vigorous growth stage of artificially cultured nitrogen-fixing Anabaena, the algae water is pumped out and filtered through a filter to separate the algae cells, and fresh nitrogen-fixing Anabaena algae mud is obtained.

[0064](2) Cell wall breaking: adjust the moisture content of the harvested algae mud to 40%, pass through a high-pressure homogenizer, break the algae cells under the conditions of a temperature of 40°C and a pressure of 2000bar, and obtain a product containing cell contents and broken cell walls Extract. Using a microscope to visually inspect, under the 800X and 1600X lenses, the extract solution is homogeneous, without cells or cell debris, and the particle size is about 400 mesh nanoparticles.

[0065] (3) Extract drying: the algae cell extract treated by the homogenizer is spray-dried under the conditions of outlet temperature 280°...

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Abstract

The invention provides an application of an anabaena and / or spirulina extract as a biostimulant in agricultural production, and belongs to the technical field of agricultural production. The anabaenaand / or spirulina extract is prepared by the following steps: carrying out wall breaking treatment on collected anabaena and / or spirulina at the temperature of 4-45 DEG C, carrying out water extraction, and carrying out spray drying on the obtained soluble algae cell extract and cell debris particles with the particle size of not more than 400 nm to obtain the anabaena and / or spirulina extract. According to the invention, the complete extraction of active components can be achieved by combining a conventional wall breaking method and a normal temperature water extraction method, so that the damage on the algae active components is avoided, the production cost is reduced, the completely water-soluble extract is obtained, the basis is provided for the subsequent preparation of the water-soluble fertilizer, and the characteristics of rapid effect and accurate fertility are provided. The application of the anabaena and / or spirulina extract as a biostimulant in agricultural production is beneficial to promoting crop growth and increasing crop yield.

Description

Technical field [0001] The invention belongs to the technical field of agricultural production, and specifically relates to the application of extracts of Anabaena and / or Spirulina as biostimulants in agricultural production. Background technique [0002] Biostimulants are a type of biologically derived products that can promote or benefit physiological processes in plants, including benefiting nutrient absorption, improving nutrient absorption efficiency and crop quality, inducing plant disease resistance and stress resistance through biological effects, and A type of substance that can improve the utilization rate of active ingredients in fertilizers and is harmless to the ecological environment. Foreign biostimulants are mainly divided into eight categories: humic acid substances, complex organic substances, beneficial chemicals, non-organic minerals, seaweed extracts, chitin, anti-transpiration agents and free amino acids. In China, biostimulant products are divided int...

Claims

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Application Information

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IPC IPC(8): C05F11/00A01N65/03A01P21/00A01G22/00A01G18/20
CPCC05F11/00A01N65/03A01G22/00A01G18/20
Inventor 秦山李运广
Owner 秦山
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