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Application of carbonized sanguisorba roots in prevention and treatment of tinea corporis and cruris and tinea manus and pedis

A technology of Burnet charcoal and tinea corporis, applied in the prevention and treatment of tinea manus and pedis, Burnet charcoal in the field of tinea corporis, can solve the problem that there are no reports on the prevention or treatment of tinea corporis and jock itch, few reports on antibacterial efficacy, and no See Trichophyton rubrum and other pathogenic bacteria of jock itch, reports on bacteriostasis, etc.

Pending Publication Date: 2020-08-11
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

("Chinese Pharmacopoeia" 2015 edition) At present, reports on the efficacy of Burnet charcoal mainly focus on hemostasis and the treatment of burns and scalds. There are few reports on antibacterial efficacy, and no antibacterial reports on pathogenic bacteria such as Trichophyton rubrum and other pathogens of tinea corporis and jock itch , and there is no report on the prevention or treatment of tinea corporis, nor such descriptions in ancient books

Method used

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  • Application of carbonized sanguisorba roots in prevention and treatment of tinea corporis and cruris and tinea manus and pedis
  • Application of carbonized sanguisorba roots in prevention and treatment of tinea corporis and cruris and tinea manus and pedis
  • Application of carbonized sanguisorba roots in prevention and treatment of tinea corporis and cruris and tinea manus and pedis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The investigation of embodiment 1 Burnet charcoal anti-trichophyton rubrum activity

[0020] The inhibitory effect of Burnet charcoal on Trichophyton rubrum was determined by the micro-liquid base dilution method. At the same time, many commonly used antibacterial traditional Chinese medicines such as Cnidium, Kochia, Saponaria, Tujingpi, Baixianpi, Sophora flavescens, etc. For comparison, Burnet burnet, the original medicinal material of Burnet charcoal, was used for comparison, and the shape change of Burnet before and after processing is shown in figure 1 .

[0021] 1. Strains

[0022] Trichophyton rubrum ATCC MYA-4438 was purchased from the American type culture collection (Americantype culture collection, ATCC).

[0023] 2. Preparation of samples to be tested

[0024] Take 10g of Cnidium Fructus Cnidii, Kochia Fructus Fructus Kochia Fructus, Saponaria japonica, Cnidaria chinensis, Cortex alba, Sophora flavescens, Burnet burnet, and Burnet charcoal powder, add 10...

Embodiment 2

[0047] Component analysis of embodiment 2 Burnet charcoal extract

[0048] The Burnet charcoal extract was prepared under item "2" in Example 1, and high performance liquid chromatography-mass spectrometry was used for component analysis, and the analysis conditions were as follows:

[0049] Chromatographic conditions: Agilent Poroshell 120EC-C18 (2.1mm×50mm, 2.7μm) chromatographic column is used, the column temperature is 30°C, acetonitrile is used as mobile phase A, water (containing 0.1% formic acid) is used as mobile phase B, according to Table 3 Gradient elution was carried out in the medium, the flow rate was 0.30 mL / min, and the injection volume was 1 μL.

[0050] Table 3 Mobile phase gradient elution program

[0051]

[0052] Primary mass spectrometry conditions: electrospray ionization (ESI) positive and negative ion mode, drying gas temperature 300°C, atomizing gas pressure 241.3kPa, capillary outlet voltage 100V, drying gas flow rate 3L / min, capillary voltage 3.5k...

Embodiment 4

[0055] Example 4 Mechanism Research of Burnet Charcoal against Trichophyton rubrum

[0056] 1. Research on the material basis of Burnet charcoal against Trichophyton rubrum

[0057] Determination of anti-redness of gallic acid, pyrogallic acid, methyl gallate, ethyl gallate, catechin, ellagic acid, ursolic acid and protocatechualdehyde in Burnet charcoal by micro-liquid dilution method Trichophyton effect, sample configuration and assay method are the same as in Example 1, and the experimental results are shown in Table 4.

[0058] Table 4 The minimum inhibitory concentration of active ingredients of Burnet charcoal to Trichophyton rubrum

[0059]

[0060]

[0061] The results show that the main material basis of Burnet charcoal against Trichophyton rubrum may be gallic acid, pyrogallic acid, methyl gallate, ethyl gallate, and protocatechualdehyde; among them, pyrogallic acid, methyl gallate The content of three components, protocatechualdehyde, in Burnet charcoal is h...

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Abstract

The invention provides application of carbonized sanguisorba roots in prevention and treatment of tinea corporis and cruris and tinea manus and pedis, and application in preparation of medicines or daily necessities. Meanwhile, the invention provides application of one or more of gallic acid, pyrogallic acid, methyl gallate, ethyl gallate and protocatechuic aldehyde in prevention and treatment oftinea corporis and cruris and tinea manus and pedis, and application in preparation of medicines or daily necessities. Trichophyton rubrum is a main pathogenic bacterium of tinea corporis and cruris and tinea manus and pedis. The invention shows that the carbonized sanguisorba roots can significantly inhibit the growth of the trichophyton rubrum, and the effect is equivalent to that of common traditional Chinese medicines sophora flavescens and golden larch bark for skin tinea. After the carbonized sanguisorba roots are administrated, the growth of the trichophyton rubrum is remarkably inhibited through ocular lens observation. Clinical curative effect observation shows that after carbonized sanguisorba root gel with the content of 0.5% is used for treating for 4 weeks, the cure rate of patients reaches 96.15%, and half of the patients are cured. Meanwhile, the main components including the gallic acid, the pyrogallic acid, the methyl gallate, the ethyl gallate and the protocatechuic aldehyde in sanguisorba roots all have a remarkable effect of resisting the trichophyton rubrum, and the content is not reported.

Description

technical field [0001] The invention belongs to the field of external application of traditional Chinese medicine, and in particular relates to the application of Burnet charcoal in the prevention and treatment of tinea corporis and tinea manus and pedis. Background technique [0002] The pathogenic fungi of tinea corporis and tinea manuum are dermatophytes, and the common feature is that they are keratinophilic. There are more than 40 species of dermatophytes, of which more than 20 species can cause diseases in humans or animals. Epidemiology data from various regions and different hospitals in China show that the pathogenic bacteria of tinea corporis, jock itch, and tinea manus and pedis are roughly the same. The most common one is Trichophyton rubrum, which is the main pathogenic bacteria. sporozoites and gypsum-like microspores, etc. Dermatophytes can be transmitted from person to person, animal to person, pollutant to person, and between different parts of the body. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/739A61K31/192A61K31/05A61K31/223A61K31/11A61P17/00A61P31/10A61K8/9789A61K8/368A61K8/34A61K8/37A61Q19/00A61Q17/00A61K125/00
CPCA61K36/739A61K31/192A61K31/05A61K31/223A61K31/11A61P17/00A61P31/10A61K8/9789A61K8/368A61K8/347A61K8/37A61Q19/00A61Q17/005A61K2236/333A61K2300/00
Inventor 顾琦梁莹陈军顾薇苏曼王昊
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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