Salmonella broad-spectrum lyase with in-vitro lytic activity and application thereof

Abstract

The invention relates to salmonella broad-spectrum lyase with in-vitro lytic activity and application thereof, and belongs to the technical field of biology. The lyase is named as LysMD19, an amino acid sequence of the lyase is shown as SEQ ID No. 1, and a nucleotide sequence of the lyase is shown as SEQ ID No. 2. The lyase can directly and efficiently lyse salmonella in-vitro under the conditionof no cell permeation reagents such as ETDA, the lysing effect is achieved on salmonella enteritidis, salmonella typhimurium, salmonella pullorum and other salmonella of different serotypes, good temperature stability and high acid and alkali resistance are achieved, the lyase can be used for preventing and treating salmonella infection, and also can be used for killing salmonella in multiple links such as source farms, processing, packaging and transportation to market selling, the safety 'from farms to dining tables' is ensured, and good application prospects and values are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a broad-spectrum lyase with in vitro lytic activity against Salmonella and its antibacterial application. Background technique [0002] Salmonella is a Gram-negative bacillus, which is not only a serious pathogen in livestock and poultry farming, but also an important zoonotic pathogen that causes food poisoning. Among all kinds of bacterial food poisoning worldwide, food poisoning caused by Salmonella often ranks first. Based on the statistical data from 2006 to 2010, Salmonella is the main pathogenic bacteria that caused outbreaks of bacterial foodborne diseases in my country ( 70%-80%), wherein the serotypes that play the main pathogenic role are Salmonella enteritidis and Salmonella typhimurium, and among the foods that cause Salmonella poisoning, meat, eggs, milk and other animal products account for about 90%. Therefore, to solve the public health problem of Salmone...

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Problems solved by technology

[0004] However, compared with Gram-positive bacteria, because the outer membrane of Gram-negative bacteria can block the effective binding of lyases to peptidoglycan targets, lyases cannot lyse Gram-negative bacteria when they are added from the outside. Lack of highly efficient lyases against Gram-negative bacteria

In order to solve the problem of Salmonella infection, researchers have expressed Lys68, LysSTP4, SPN1S, gp146, Gp110, Lys394, PsP3gp10, LysT144 and other lyases targeting Salmonella. These lyases can effectively Lyse Salmonella; but unfortunately, without the help of cell permeabilizers, these lytic enzymes have no cleavage activity in vitro, and cannot play a bactericidal effect when used as antibacterial drugs to directly kill bacteria; and cell permeabilizers such as EDTA have certain toxicity , has a stimulating effect on mucous membranes, upper respiratory tract, etc., so this type of lyase is not suitable for use as antibacterial drugs, feed additives, etc.

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