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Molecular logic gate with three-phase output function for quickly identifying Ag<+> and Cys, and construction method of molecular logic gate

A logic gate and three-phase technology, applied in the field of three-phase output molecular logic gate and its construction, can solve the problem of low cost

Active Publication Date: 2020-05-08
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the limitations of the single signal output of existing molecular logic gates, and provides a method for quickly identifying Ag + A molecular logic gate and a construction method thereof with a three-phase output function of Cys, the signal output in the construction method of the molecular logic gate is a combined output of fluorescence intensity, fluorescence anisotropy, and fluorescence attenuation three-phase signals, providing more reliable and sensitive At the same time, the nucleic acid aptamer does not need to be labeled, the analysis and detection are simple and the cost is lower

Method used

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  • Molecular logic gate with three-phase output function for quickly identifying Ag&lt;+&gt; and Cys, and construction method of molecular logic gate
  • Molecular logic gate with three-phase output function for quickly identifying Ag&lt;+&gt; and Cys, and construction method of molecular logic gate
  • Molecular logic gate with three-phase output function for quickly identifying Ag&lt;+&gt; and Cys, and construction method of molecular logic gate

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Experimental program
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Effect test

Embodiment 1

[0048] The first step is to prepare berberine mother liquor:

[0049] Weigh 74.3mg of berberine hydrochloride, and dilute it with secondary deionized water in a 100 mL volumetric flask;

[0050] In the second step, Ag + -Preparation of nucleic acid aptamer solution:

[0051] Ag + - The aptamer was centrifuged for 5-10min, and the centrifuged Ag + -Nucleic acid aptamer was dissolved in 20 mmol / L Tris-HAc buffer to prepare Ag with a concentration of 100 μM + -Nucleic acid aptamer solution, heated at 90°C for 7 minutes, cooled to room temperature, stored at -20°C for later use, and diluted to 10 μM with 20 mmol / L Tris-HAc buffer before use;

[0052] The third step is to prepare Ag with different concentration gradients + Ionic standard solution:

[0053] Weigh 67.9 mg of silver nitrate, and dilute it in a 100 mL volumetric flask with secondary deionized water;

[0054] The fourth step is to prepare Cys standard solutions with different concentration gradients:

[0055] We...

Embodiment 2

[0070] The first step is to prepare berberine mother liquor:

[0071] Weigh 74.3mg of berberine hydrochloride, and dilute it with secondary deionized water in a 100 mL volumetric flask;

[0072] In the second step, Ag + -Preparation of nucleic acid aptamer solution:

[0073] Ag + - The aptamer was centrifuged for 5-10min, and the centrifuged Ag + -Nucleic acid aptamer was dissolved in 20 mmol / L Tris-HAc buffer to prepare Ag with a concentration of 100 μM + -Nucleic acid aptamer solution, heated at 90°C for 7 minutes, cooled to room temperature, stored at -20°C for later use, and diluted to 10 μM with 20 mmol / L Tris-HAc buffer before use;

[0074] The third step is to prepare Ag with different concentration gradients + standard solution:

[0075] Weigh 67.9 mg of silver nitrate, dilute it in a 100 mL volumetric flask with secondary deionized water, and dilute step by step to obtain standard solutions of different concentrations;

[0076] The fourth step is to prepare Cys...

Embodiment 3

[0095] The first step is to prepare berberine mother liquor:

[0096] Weigh 74.3mg of berberine hydrochloride, and dilute it with secondary deionized water in a 100 mL volumetric flask;

[0097] In the second step, Ag + -Preparation of nucleic acid aptamer solution:

[0098] Ag + - The aptamer was centrifuged for 5-10min, and the centrifuged Ag + -Nucleic acid aptamer was dissolved in 20 mmol / L Tris-HAc buffer to prepare Ag with a concentration of 100 μM + -Nucleic acid aptamer solution, heated at 90°C for 7 minutes, cooled to room temperature, stored at -20°C for later use, and diluted to 10 μM with 20 mmol / L Tris-HAc buffer before use;

[0099] The third step is to prepare Ag with different concentration gradients + standard solution:

[0100] Weigh 67.9 mg of silver nitrate, dilute it in a 100 mL volumetric flask with secondary deionized water, and dilute step by step to obtain standard solutions of different concentrations;

[0101] The fourth step is to prepare Cys...

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Abstract

The invention discloses a molecular logic gate with a three-phase output function for quickly identifying Ag<+> and Cys and a construction method of the molecular logic gate, which belong to the technical field of molecular computers, and can solve the problem of limitation of single signal output of an existing molecular logic gate. The molecular logic gate is characterized in that: Cys and Ag<+>are taken as two parallel input signals, and fluorescence intensity, fluorescence anisotropy and fluorescence lifetime are taken as output signals; and an ''inhibit '' gate, namely, a basic Boolean logic gate is constructed by taking berberine as a signal report unit and an Ag<+>-aptamer as a molecular recognition matrix and utilizing changes of fluorescence intensity, fluorescence anisotropy andfluorescence attenuation of the berberine in different microenvironments in the presence of the Ag<+>-aptamer, Cys and Ag<+>. The constructed molecular logic gate circuit is good in performance, highin sensitivity and easy to distinguish, and can solve the limitation caused by a single output signal.

Description

technical field [0001] The invention belongs to the technical field of molecular computers, and in particular relates to a rapid identification of Ag based on nucleic acid aptamer-target interaction and metal ion coordination. + Molecular logic gates with three-phase output function of Cys and its construction method. Background technique [0002] As a chemical system simulating logic operations, molecular logic gates realize the miniaturization of information processing and integration through computation at the molecular level. Similar to logic gates in digital circuits, like silicon processors that use electronic motion, to create micro-systems that can use the inherent diversity of molecules to complete computing functions, use molecules to describe the input and output signals of logic gates, and then realize Logical operations at the molecular level. Molecules in molecular logic gates can respond to external stimuli, and molecular logic gates also follow the rules of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06G06N3/00
CPCC09K11/06G01N21/6428G01N2021/6439G06N3/002
Inventor 卫艳丽兰艺凤秦国杰王丽董川
Owner SHANXI UNIV
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