Fluorescent probe for quantitatively detecting acidic or basic amino acids on basis of carbon quantum dot fluorescence quenching or enhancement method and preparation method for fluorescent probe

A carbon quantum dot and fluorescence quenching technology, applied in the field of fluorescent probes, can solve the problems of slow growth, mental retardation, stunting, etc., and achieve the effects of low price, simple preparation method, and easy procurement.

Active Publication Date: 2020-05-08
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The lack of amino acids or amino acid imbalance in children will affect the body's abso

Method used

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  • Fluorescent probe for quantitatively detecting acidic or basic amino acids on basis of carbon quantum dot fluorescence quenching or enhancement method and preparation method for fluorescent probe
  • Fluorescent probe for quantitatively detecting acidic or basic amino acids on basis of carbon quantum dot fluorescence quenching or enhancement method and preparation method for fluorescent probe
  • Fluorescent probe for quantitatively detecting acidic or basic amino acids on basis of carbon quantum dot fluorescence quenching or enhancement method and preparation method for fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation and Characterization of C-dots

[0041] Step 1: Accurately weigh 0.05 g of o-phenylenediamine and 0.05 g of p-aminobenzoic acid, add 10 mL of absolute ethanol to them, dissolve them fully by ultrasonication (200W, 10 min), and transfer them to 100 mL of polytetrafluoroethylene lining , assemble the reactor and put it in the oven, at 180 o C for 12 h.

[0042] Step 2: After the reactor is cooled to room temperature, the solution in the reactor is rotatably evaporated to remove absolute ethanol. Add 10 mL of secondary water, sonicate (200W, 10 min) to dissolve the sticky matter and transfer it to a centrifuge tube, centrifuge the solution at 8000 rpm for 10 minutes, transfer the supernatant to a beaker, and freeze-dry to obtain C-dots solid powder.

[0043] Step 3: Weigh 0.1 g of C-dots solid powder into a beaker, add 10 mL of secondary water to it, and ultrasonically (200W, 10 min) dissolve it fully to obtain a C-dots stock solution with a conce...

Embodiment 2

[0046] Example 2: Response of different substances to C-dots

[0047] Step 1, accurately weigh a certain mass of metal ions (Sn 2+ , Fe 3+ , Al 3+ , Cr 3+ , Fe 2+ , Hg 2+ , Ag + , Mn 2+ , Ba 2+ , Co 2+ , Cu 2+ , Zn 2+ , Na + , Ca 2+ , Ni 2+ , Mg 2+ , Cr(VI), Pb 2+ ) compound, add 10 mL of secondary water, ultrasonically (200W, 5 min) fully dissolve, and prepare a metal ion stock solution with a molar concentration of 0.1 mol / L.

[0048] Step 2: Accurately weigh a certain mass of amino acids (aspartic acid, glutamic acid, tryptophan, phenylalanine, methionine, cysteine, proline, isoleucine, serine, valine amino acid, threonine, leucine, glycine, alanine, homocysteine, tyrosine, asparagine, glutamine, histidine, arginine, lysine), adding 10 mL of secondary water, ultrasonically (200W, 5 min) to fully dissolve, and prepare an amino acid stock solution with a molar concentration of 0.01 mol / L.

[0049] Step 3, accurately weigh a certain mass of anion (C 2 o 4 ...

Embodiment 3

[0056] Example 3: C-dots for titration of acidic and basic amino acids

[0057] Step 1, add 7 µL of C-dots (10 mg / mL) stock solution to 2 mL of secondary water, at this time the concentration of C-dots is 0.0349 mg / mL (2007 µL), test and record the fluorescence intensity of C-dots f 0 .

[0058] Step 2, add aspartic acid stock solution drop by drop to the solution in step 1, test and record the fluorescence intensity F of C-dots, draw the fluorescence spectrum diagram of the whole titration process, see the experimental results Figure 9 . Calculation (F 0 -F) / F 0 And take it as the ordinate, the concentration of aspartic acid as the abscissa, utilize Origin fitting to obtain the linear equation, (F 0 -F) / F 0 = 0.0080c (Asp) +0.0699, R 2 =0.9989, the linear range is 10.94-29.80 μmol / L, and the lowest detection limit is 0.1925 umol / L. See the experimental results Figure 10 .

[0059] Step 3, add glutamic acid stock solution drop by drop to the solution in step 1, ...

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Abstract

The invention belongs to the technical field of fluorescent probes, and provides a fluorescent probe for quantitatively detecting acidic or basic amino acids on basis of a carbon quantum dot fluorescence quenching or enhancement method and a preparation method for the fluorescent probe. The preparation method comprises the following steps: with o-phenylenediamine and p-aminobenzoic acid as substrates, synthesizing carbon quantum dots (C-dots) through a hydrothermal method, and performing rotary evaporation, centrifuging and freeze-drying so as to obtain the fluorescent probe--C-dots solid powder. C-dots are sensitive to pH; when the pH is acidic, fluorescence of the C-dots is weakened; and when the pH is alkaline, the fluorescence of the C-dots is enhanced. Thus, the C-dots are used for amino acid category detection. When acidic amino acid is added, the fluorescence of the C-dots is gradually weakened; when basic amino acid is added, the fluorescence of the C-dots is gradually enhanced; and the C-dots can be used for acidic and basic amino acid detection. The method provided by the invention has the advantages of simple operation, good selectivity, high sensitivity, rapid detection, no need of expensive instruments and equipment, and low detection cost, and can be used for qualitative distinguishing detection of acidic and alkaline amino acids.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to a fluorescent probe for quantitatively detecting acidic or basic amino acids based on a carbon quantum dot fluorescence quenching or enhancement method and a preparation method thereof. Background technique [0002] Amino acid is one of many biologically active macromolecules that build biological organisms, and is the basic material for building cells and repairing tissues. The most common amino acids in the human body are glycine (Gly), alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), phenylalanine (Phe), proline Amino acid (Pro), Tryptophan (Trp), Serine (Ser), Tyrosine (Tyr), Cysteine ​​(Cys), Methionine (Met), Asparagine (Asn), Glutamine Amide (Gln), Threonine (Thr), Aspartic Acid (Asp), Glutamic Acid (Glu), Lysine (Lys), Arginine (Arg) and Histidine (His). According to the difference of the functional groups contained on the surface of ...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65G01N21/6428G01N2021/6432G01N2021/6439
Inventor 弓晓娟吴壮壮刘洋宋胜梅董川
Owner SHANXI UNIV
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