A kind of low fibroin scaffold and preparation method thereof

A protein scaffold and silk protein technology, which is applied in the field of biomedicine, can solve the problems of inability to prepare low-crystallinity water-insoluble silk protein scaffolds, etc., and achieves the effects of good hot water stability, mild conditions and simple process.

Active Publication Date: 2021-09-28
上海丝波敦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a novel silk protein scaffold and its preparation method, which overcomes the problem that the prior art cannot prepare low-crystallinity water-insoluble silk protein scaffolds

Method used

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  • A kind of low fibroin scaffold and preparation method thereof
  • A kind of low fibroin scaffold and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Soak about 10g raw silk in 0.5L 0.5% Na 2 CO 3 solution, stirred and boiled for 60 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0036] 5 g of the degummed silk after the above treatment was weighed and dissolved in 30 mL of LiBr solution with a concentration of 9.8 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut off of 3500 to dialyze with deionized water for 3 days to obtain a silk protein aqueous solution with a concentration of 6%, and further adjust the concentration of the silk protein aqueous solution to 2%.

[0037] Take 10 mL of the above silk protein solution, add 5 mL of 0.5% ethylene glycol solution, stir evenly, pour it into a polyethylene mold, and then put the mold containing the mixed solution into -20 ° C for 6 hours to freeze. Then take out the frozen body, put it into deionized water to thaw for 1 hour, then rin...

Embodiment 2

[0040] Soak about 50g raw silk in 2L 0.05% Na 2 CO 3 solution, stirred and boiled for 60 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0041] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 9.8 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 7%, and adjust the concentration of the silk protein aqueous solution to 4%.

[0042] Take 15 mL of the above silk protein solution, blend it with 15 mL of ethanol with a mass fraction of 0.1%, and then pour it into a polyethylene mold and freeze it at -20°C for 10 hours. After being frozen, it was taken out, thawed in the air at natural temperature for 2 hours, rinsed with deionized water for 3 hou...

Embodiment 3

[0045] Soak about 50g raw silk in 2L 0.05% Na 2 CO 3 solution, stirred and boiled for 60 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0046] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 9.8 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 7%, and adjust the concentration of the silk protein aqueous solution to 5%.

[0047] Take 15 mL of the above silk protein solution, blend it with 30 mL of methanol with a mass fraction of 0.01%, and then pour it into a polyethylene mold and freeze it at -20°C for 12 hours. After being frozen, it was taken out, thawed in the air at natural temperature for 1 hour, rinsed with deionized water for 3 ho...

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Abstract

The invention relates to a method for preparing a low-crystal silk protein scaffold, comprising the following steps: uniformly blending an aqueous solution of silk protein and an aqueous solution of low-concentration small-molecule alcohol to obtain a mixed solution of silk protein, and using small-molecule alcohol to induce silk protein molecules to entangle and At the same time, the low concentration of small molecule alcohols is used to avoid the transformation of the silk protein conformation; then the silk protein mixed solution is frozen to obtain the silk protein frozen body, and the freezing process is used to promote the occurrence of phase separation; and then the silk protein frozen body Thawing uses the melting of the ice core to form a three-dimensional porous scaffold with a uniform structure; finally, a dry low-crystalline fibroin porous scaffold that is easy to store is prepared through a freeze-drying process.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a low fibroin scaffold and a preparation method thereof. Background technique [0002] There are millions of patients with organ or tissue damage and functional loss due to diseases and accidents every year. In the United States alone, more than 8 million operations are needed to treat such patients every year, and the economic cost is more than 400 billion US dollars. With the development of modern medicine and surgical techniques, tissue or organ transplantation to repair functional loss has been widely accepted, however, it faces a huge donor gap. The formation of tissues or organs in vivo or in vitro by means of regenerative medicine provides a new treatment option for the repair of damaged functions. Among them, the selection and construction of tissue engineering scaffold materials become one of the keys to this treatment method. [0003] Silk protein is a natural polymer bioma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/56A61L27/58
CPCA61L27/227A61L27/50A61L27/56A61L27/58C08L89/00
Inventor 张锋路青青陆福男范志海秦建忠张鹏高洋陆史俊左保齐
Owner 上海丝波敦生物科技有限公司
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