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Method for producing acetone-ethanol-butanol (ABE) by fermentation with straw hydrolysate as raw material

A technology of hydrolyzate and straw, applied in the field of biomass energy preparation, can solve the problems of lack of ability to synthesize glutathione, few research reports on anaerobic bacteria, etc., achieve good practical value and promotion and application significance, and improve fermentation yield The effect of rate and dosage optimization

Active Publication Date: 2020-01-21
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] Changes in the oxidation-reduction environment in the fermentation broth have a great impact on cell growth and product fermentation. Glutathione is often used to regulate the oxidation-reduction potential of aerobic bacteria. There are few research reports on anaerobic bacteria
Clostridium acetobutylicum and Clostridium beijejerinckii are anaerobic bacteria that do not have the ability to synthesize glutathione

Method used

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  • Method for producing acetone-ethanol-butanol (ABE) by fermentation with straw hydrolysate as raw material
  • Method for producing acetone-ethanol-butanol (ABE) by fermentation with straw hydrolysate as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The method for producing ABE by fermenting the straw hydrolyzate provided in this example with fresh corncobs as the raw material for fermentation is as follows.

[0044] (1) Preparation of straw hydrolyzate

[0045] When preparing the straw hydrolyzate, steps such as straw pretreatment and biological enzymolysis are included, specifically:

[0046] (1) Straw pretreatment

[0047] After drying the fresh corncobs, crush them through a 40-mesh sieve, put 20g of corncobs into a 500mL conical flask, add 200mL of 0.75% dilute sulfuric acid, and treat them at 121°C for 60min, Ca(OH) 2 Adjust the pH to 4.8.

[0048] (2) Biological enzymatic hydrolysis

[0049] Add biological enzymes to the sterilized straw mixture in step (1), mix well, enzymatically treat at 55°C for 48h, then centrifuge at 3000r / min for 5min to remove solid sediment, and the supernatant is the hydrolyzed straw to be fermented liquid;

[0050] The biological enzyme is a mixture of cellulase, xylanase, an...

Embodiment 2

[0066] In this example, fresh corncobs were taken as an example to conduct a fermentation experiment. The specific operation was the same as in Example 1, only the strain was adjusted to Clostridium acetobutylicum (the preparation method of the seed liquid was the same as in Example 1), and the addition of glutathione was set. The time node is added after 8 hours of fermentation (the amount added is the same as in Example 1).

[0067] The total RNA was extracted at 18h in the acidogenic period and 36h in the solventogenic period, and the difference in gene expression of key enzymes in the solvent metabolism pathway was analyzed by fluorescent quantitative PCR method. The fluorescent quantitative results were as follows: figure 2 Shown (specific key enzyme genes and corresponding enzyme names are: ack acetate kinase; buk butyrate kinase; ptb phosphotransbutyrylase; pta phosphotransacetylase; adhE alcohol dehydrogenase; ctfAB CoA transferase; bdhB butanol dehydrogenase Hydrogen...

Embodiment 3

[0073] In this example, taking fresh wheat straw as an example, a fermentation experiment was carried out. The specific operation is the same as that in Example 1. Clostridium acetobutylicum and Clostridium beijerinckii were used for fermentation respectively, and the time node for adding glutathione was set as after 8 hours of fermentation Add (the amount added is the same as in Example 1). the result shows:

[0074] When inoculating Clostridium acetobutylicum alone for fermentation, in the fermentation broth: ethanol content 1.25g / L, acetone content 3.17g / L, butanol content 4.89g / L, total solvent content 9.31g / L;

[0075] When inoculated with Clostridium beijerinckii for fermentation alone, in the fermentation broth: ethanol content 1.03g / L, acetone content 2.64g / L, butanol content 4.32g / L, total solvent content 7.99g / L.

[0076] As a control, if glutathione is not added (other related operations are the same), after 72 hours of fermentation, when Clostridium acetobutylicum...

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Abstract

The invention belongs to the technical field of biomass energy preparation, and particularly relates to a method for producing acetone-ethanol-butanol (ABE) by fermentation with a straw hydrolysate asa raw material. The method specifically includes the steps of straw pretreatment, biological enzymolysis, preparation of an ABE fermentation culture solution, a fermentation hydrolysate and the like.According to the method, when butanol is fermented with a straw acid hydrolysate as a raw material and in combination with the fermentation treatment of specific microbial strains, the expression level of related key enzymes in a solvent metabolism pathway can be significantly affected by adding glutathione, and thus chemical compositions of cell membranes of the strains are changed, and the butanol production capacity is improved. According to the method, straws are pretreated into hydrolysate and then directly fermented to produce ABE, the complex detoxification process is avoided, so thatthe production cost is reduced, and the industrialization of ABE fermentation is promoted; and furthermore, through the optimization of the fermentation process, especially the addition and utilization of the glutathione in the fermentation process, the fermentation yield of the ABE is further improved, and the ABE industrial production is promoted.

Description

technical field [0001] The application belongs to the technical field of biomass energy preparation, and specifically relates to a method for producing ABE by fermenting straw hydrolyzate as a raw material. Background technique [0002] In the development and utilization of biomass energy technology, acetone-ethanol-butanol (ABE) fermentation is a relatively mature fermentation technology and the second largest fermentation process in the world after alcohol fermentation. With the rapid development of the global economy, energy issues have become an important constraint on economic development, and it is imminent to find potential energy sources that can replace fossil fuels. [0003] Bio-fermentation to produce fuel is one of the most effective methods, and bio-butanol is one of the most promising researches on bio-fuel. Biobutanol is not only an important organic chemical raw material, but also a new type of biofuel with great potential. However, the use of food crops to...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P7/16C12P7/36C12P19/14C12P19/02C12N1/38C12R1/145
CPCC12P7/10C12P7/16C12P7/36C12P19/14C12P19/02C12N1/38C12P2203/00C12P2201/00Y02E50/10
Inventor 郜晓峰苏亚蕊贺洁马芳园王深垒
Owner HENAN UNIVERSITY
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