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Macromolecular potassium ion fluorescent probe as well as preparation method and application thereof

A fluorescent probe, potassium ion technology, applied in fluorescence/phosphorescence, chemical instruments and methods, analytical materials, etc., can solve the problems of the probe detection limit range to be further improved, the poor water solubility of small molecule probes, etc., to achieve water solubility And the effect of improving biocompatibility and high selectivity

Active Publication Date: 2020-01-03
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the water solubility of this small molecule probe is poor, and the detection limit range of this type of probe needs to be further improved

Method used

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  • Macromolecular potassium ion fluorescent probe as well as preparation method and application thereof
  • Macromolecular potassium ion fluorescent probe as well as preparation method and application thereof
  • Macromolecular potassium ion fluorescent probe as well as preparation method and application thereof

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Embodiment 1

[0074] This embodiment provides a preparation method of polymer potassium ion fluorescent probe P1, specifically as follows:

[0075] (1) Synthetic polymer OEG-1

[0076] Polyethylene glycol monomethyl ether methyl methacrylate (mPEG350-MMA, 1g), methacrylic acid (MAA, 60mg), methacryloyloxyethyltrimethylammonium chloride (METAC, 50mg), porphyrin Porphylin monomer (Porphylin-MA, 2mg) and azobisisobutyronitrile (AIBN, 20mg) were added into a 10mL Shrek (Schlenk) bottle, and 5mL DMF was added to dissolve. Freeze the mixed liquid into a solid state with liquid nitrogen. After repeated vacuuming and thawing three times, nitrogen gas was passed, the valve was closed, and the reaction was carried out in an oil bath at 65°C for 16 hours. After the reaction was completed, the reaction solution was slowly dropped into methanol under ice-bath conditions to obtain a white precipitate. Suction filtration and rinsing with methanol three times gave white solid OEG-1 (959.0 mg), the calcu...

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Abstract

The invention relates to a macromolecular potassium ion fluorescent probe as well as a preparation method and application thereof. The probe is a ratio type macromolecular potassium ion fluorescent probe based on a water-soluble macromolecular skeleton, has a structure as shown in P1 and is prepared by the steps of performing active free radical polymerization reaction to obtain macromolecular OEG-1 which is completely water soluble, performing esterification reaction on carboxyl in a macromolecular skeleton and hydroxyl in a micromolecular potassium ion probe KS-23 and grafting a potassium ion sensor KS-23 on the water-soluble macromolecular skeleton to obtain the ratio type macromolecular potassium ion fluorescent probe P1 based on the water-soluble macromolecular skeleton. Compared withthe micromolecular potassium ion KS-23, the water solubility and the biocompatibility of the P1 are improved, the potassium ion selectivity is high, the potassium ion detection range is between 1 and200 mM, the potassium ion concentration is expected to be subjected to quantitative analysis through peak ratio, and the requirement on intracellular potassium ion quantitative detection is met.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a polymer potassium ion fluorescent probe and its preparation method and application. Background technique [0002] Potassium ion (K + ) is one of the most abundant metal ions in mammalian cells, accounting for about 4% of the dry weight of the human body. It plays an extremely important role in many physiological activities, including heart beat, muscle contraction, nerve signal conduction and renal excretion. K + It is not only involved in maintaining intracellular osmotic pressure and electrolyte balance, regulating blood pressure, but also involved in regulating the transduction of biochemical signals in the entire nervous system. K in the cytoplasm of mammalian cells + The concentration of ions is about 130-180mM, while the extracellular concentration is 3.5-5.0mM. Potassium ion levels in normal serum have been maintained between 3.5-5.5mM, when excessive i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/28C08F220/06C08F220/34C08F8/42C09K11/06G01N21/64
CPCC08F8/42C08F220/28C09K11/06C09K2211/14G01N21/6428
Inventor 田颜清宁爵伟
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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