Macromolecular potassium ion fluorescent probe as well as preparation method and application thereof
A fluorescent probe, potassium ion technology, applied in fluorescence/phosphorescence, chemical instruments and methods, analytical materials, etc., can solve the problems of the probe detection limit range to be further improved, the poor water solubility of small molecule probes, etc., to achieve water solubility And the effect of improving biocompatibility and high selectivity
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[0074] This embodiment provides a preparation method of polymer potassium ion fluorescent probe P1, specifically as follows:
[0075] (1) Synthetic polymer OEG-1
[0076] Polyethylene glycol monomethyl ether methyl methacrylate (mPEG350-MMA, 1g), methacrylic acid (MAA, 60mg), methacryloyloxyethyltrimethylammonium chloride (METAC, 50mg), porphyrin Porphylin monomer (Porphylin-MA, 2mg) and azobisisobutyronitrile (AIBN, 20mg) were added into a 10mL Shrek (Schlenk) bottle, and 5mL DMF was added to dissolve. Freeze the mixed liquid into a solid state with liquid nitrogen. After repeated vacuuming and thawing three times, nitrogen gas was passed, the valve was closed, and the reaction was carried out in an oil bath at 65°C for 16 hours. After the reaction was completed, the reaction solution was slowly dropped into methanol under ice-bath conditions to obtain a white precipitate. Suction filtration and rinsing with methanol three times gave white solid OEG-1 (959.0 mg), the calcu...
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