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A kind of fiber material and its preparation method and application

A fiber material and fiber technology, which is applied in the field of fiber material and its preparation and application, can solve the problems of complicated loading and release process, limit practical application, etc., and achieve the effects of not easy to degrade and fail, simple loading method and high transfer efficiency.

Active Publication Date: 2022-05-10
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The load and release process of this method is relatively complicated, which limits its practical application

Method used

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  • A kind of fiber material and its preparation method and application
  • A kind of fiber material and its preparation method and application
  • A kind of fiber material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) The steps for synthesizing GNR are as follows: first, prepare its seed solution, mix hexadecyltrimethylammonium bromide (CTAB) (0.1M, 9.75mL), chloroauric acid (HAuCl 4 ) (0.01M, 0.25mL), NaBH 4 (0.01M, 0.6mL) was mixed and stirred for 2min, and then used after standing for at least 2h. 4 (0.01M, 0.4mL), AgNO 3 (0.01M, 80μL), ascorbic acid (0.1M, 64μL), add HCl (1.0M, 160μL) and mix well; finally, add 17μL of prepared seed solution to 8.7mL growth solution, leave it overnight at 28°C, and The centrifuge was used after centrifuging at 8000 rpm to remove impurities, and the aspect ratio of the prepared GNR was 4.08.

[0048] (2) Prepare the outer layer spinning solution: dissolve PLLA (2.8g), gelatin (200mg), and GNR (15.6mg) in 20mL of polyhexafluoroisopropanol (HFIP), stir overnight into a colorless viscous shape , take out 5mL as the outer spinning solution.

[0049] (3) Configure the inner layer spinning solution: the bFGF plasmid encoding GFP green fluoresce...

Embodiment 2

[0056] The preparation method of the electrospun fiber is the same as that of Example 1 when the N / P is 30. The SEM and TEM figures of the electrospun fiber (expressed in PG / GNR) obtained in this embodiment are shown in image 3 , wherein, (a) SEM characterization of PG / GNR core-shell structure spun fibers, scale bar: 5 μm. (b) TEM characterization of PG / GNR core-shell structure spun fibers and GNRs. GNRs are uniformly distributed in the shell of spun fibers. Scale bar: 20nm, 200nm.

[0057] The cell experiment method is basically the same as in Example 1, the difference is that: the laser light source with a wavelength of 808nm is used at a rate of 0.45W / cm 2 The power density in the hole was irradiated for different times to measure the photothermal effect of the electrospun fiber. The measurement results are shown in Figure 4 (Wet state), it can be seen from the results that the surface temperature of the spun fiber rises rapidly, and the temperature reaches about 45°C a...

Embodiment 3

[0059] The preparation method of the electrospun fiber is the same as in Example 2.

[0060] The electrospun fibers prepared in this example were subjected to cell culture according to the method of Example 1 (ie steps (5) to (7) of Example 1).

[0061] The sample processing of the cultured cells is divided into three parts, which are (1) flow cytometry to characterize the transfection, (2) cell proliferation experiment, and (3) cell migration experiment.

[0062] (1) Characterization of transfection by flow cytometry: After 48 hours of laser irradiation, the cells cultured on the fiber were digested with trypsin, centrifuged, resuspended in sterile PBS and placed in a flow tube, and placed in a flow tube without any Treated NIH-3T3 cells were used as a control group for flow cytometry testing, and the test results are shown in Figure 5 . The results of flow cytometry to characterize the transfection efficiency show that the electrospun fiber material described in the prese...

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Abstract

The invention relates to a fiber material, which comprises a fiber core layer and a fiber shell layer coated outside the fiber core layer, the fiber core layer includes exogenous molecules and polyethyleneimine; the fiber shell Layers include gold nanorods and biodegradable materials. The fiber material of the present invention achieves the dual purposes of release and efficient delivery of exogenous molecules, and the loading method of exogenous molecules is simple, the delivery efficiency is high, and it is not easy to be degraded and invalidated before release, and has potential applications in biomedical fields such as tissue engineering value.

Description

technical field [0001] The invention specifically relates to a fiber material and its preparation method and application. Background technique [0002] Gene delivery is an important link in gene therapy and tissue engineering. Among these, surface-mediated gene delivery stands out as an even greater advantage. Different from traditional gene delivery methods, this type of research loads gene molecules on the surface or body of biological materials. When the material contacts cells in vitro or implanted into the body, the genes at the surface interface of the material will be endocytized by the cells to realize gene expression. transfer. Compared with other traditional delivery methods, surface-mediated gene delivery has irreplaceable advantages such as high transfection efficiency, stable vector, and long-term gene release. [0003] In recent years, functional genes with regulatory functions are embedded or loaded on the surface of biomaterials and devices, and the functi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/16D01F8/18D01F8/02D01F8/14D01F1/10A61L31/06A61L31/04A61L31/02A61L31/14A61L31/16
CPCD01F8/16D01F8/18D01F8/02D01F8/14D01F1/10A61L31/06A61L31/045A61L31/022A61L31/148A61L31/16A61L2400/04A61L2300/258A61L2300/252A61L2300/414C08L79/02C08L67/04
Inventor 于谦郑琰君张燕霞周洋
Owner SUZHOU UNIV
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