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Fish cartilage extract for preventing arthritis

A fishbone extract, a technology for extracting, applied in the directions of anti-inflammatory agents, bone diseases, drug combinations, etc., can solve the problems of hindering the passage of gastric and intestinal mucosa, many hydrophilic groups, incomplete absorption, etc., and achieve the preparation method. Simple and feasible, ensure anti-complement activity, reduce the effect of loss

Inactive Publication Date: 2019-11-22
浦江县昂宝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chondroitin sulfate is an important component of articular cartilage. It can up-regulate the expression level of type II collagen mRNA in chondrocytes and promote cartilage regeneration. It was found that the relative molecular weight of chondroitin sulfate is large, and its large volume often prevents it from passing through the gastric and intestinal mucosa smoothly, so it cannot perform its various pharmacological functions well in the body, and it has many hydrophilic groups and is fat-soluble. Poor, so incomplete absorption in the body, and low molecular weight chondroitin sulfate (LMWCS) has the characteristics of low viscosity, good solubility, easy absorption and high bioavailability, can pass through the gastric and intestinal mucosa smoothly, and has better biological Utilization and therapeutic effect of type II collagen-induced rheumatoid arthritis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Fish cartilage extract for preventing arthritis, the fish bone extract contains 80-90% low molecular weight chondroitin sulfate and 10-20% collagen peptide, the molecular weight of the above low molecular weight chondroitin sulfate is 400-500Da. The above-mentioned fish cartilage extract prevents bone damage caused by degenerative osteoarthritis or wear and tear due to joint aging, and can be used as a candidate component for preparing health food for preventing, treating or improving arthritis diseases.

[0014] The preparation method of the fish cartilage extract that prevents arthritis specifically comprises the following steps:

[0015] 1) Preparation of fish cartilage powder: Wash the fish cartilage, add distilled water to the fish cartilage according to the material-liquid ratio of 1:15 (g / mL), then add acarbose with a weight of 0.03‰ of fish cartilage and fish cartilage with a weight of 0.3 % vitamin C, soaked for 1 hour, boiled for 40 minutes, fished out and rem...

Embodiment 2

[0019] Fish cartilage extract for preventing arthritis, the fish bone extract contains 80-90% low molecular weight chondroitin sulfate and 10-20% collagen peptide, the molecular weight of the above low molecular weight chondroitin sulfate is 400-500Da.

[0020] The preparation method of the fish cartilage extract that prevents arthritis specifically comprises the following steps:

[0021] 1) Preparation of fish cartilage powder: Wash the fish cartilage, add distilled water to the fish cartilage according to the material-to-liquid ratio of 1:10 (g / mL), then add acarbose with a weight of 0.05‰ of fish cartilage and fish cartilage with a weight of 0.2 % vitamin C, soaked for 3 hours, boiled for 20 minutes, removed fish meat and grease, rinsed with water, dried, coarsely crushed to 50 mesh, and then ultrafinely crushed in an ultrafine pulverizer to obtain fish cartilage powder. spare;

[0022] 2) Enzymatic hydrolysis extraction: add distilled water to fish cartilage powder accord...

Embodiment 3

[0025] Fish cartilage extract for preventing arthritis, the fish bone extract contains 80-90% low molecular weight chondroitin sulfate and 10-20% collagen peptide, the molecular weight of the above low molecular weight chondroitin sulfate is 400-500Da.

[0026] The preparation method of the fish cartilage extract that prevents arthritis specifically comprises the following steps:

[0027] 1) Preparation of fish cartilage powder: Wash the fish cartilage, add distilled water to the fish cartilage according to the material-liquid ratio of 1:12 (g / mL), then add acarbose with a weight of 0.04‰ of fish cartilage and 0.25 weight of fish cartilage % vitamin C, soaked for 2 hours, boiled for 30 minutes, fished out, fish meat and oil removed, rinsed with water, dried, coarsely crushed to 30 mesh, and then ultrafinely crushed in an ultrafine pulverizer to obtain fish cartilage powder. spare;

[0028] 2) Enzymatic hydrolysis and extraction: add distilled water to fish cartilage powder ac...

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Abstract

The invention discloses a fish cartilage extract for preventing arthritis. The fish cartilage extract is prepared from 80-90% of low-molecular-weight chondroitin sulfate and 10-20% of collagen peptide, and the molecular weight of the low-molecular-weight chondroitin sulfate is 400-500 Da. A preparation method of the fish cartilage extract includes the following steps: fish cartilages are soaked indistilled water and then boiled, fish meat and grease are removed, drying is conducted, and superfine grinding is conducted to obtain fish cartilage powder; distilled water and a complex enzyme are added into the fish cartilage powder, enzymolysis, enzyme killing, centrifugation and ultra-filtration are conducted, and then drying is conducted to obtain ultra-filtration powder; and distilled waterand a chondroitin sulfate AC enzyme are added into the ultra-filtration powder for enzymatic degradation, enzyme killing, filtration, ultra-filtration, concentration and dialysis are conducted, and freeze drying is conducted to obtain the fish cartilage extract. The fish cartilage extract has the beneficial effects that the fish cartilage extract has the effect of anticomplement activation, can protect chondrocytes against being cracked and has the better potential curative effect in prevention and treatment of arthritis.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to fish cartilage extracts for preventing arthritis. Background technique [0002] my country's fish output ranks first in the world, with an annual output of nearly 40 million tons. In the general aquatic product processing process, fish cartilage and discarded fish heads are often used as leftovers, or processed into fish bone meal and added to poultry feed, but more Fish cartilage and fish heads are discarded, causing environmental pollution and waste of resources. The structure of fish cartilage is relatively loose, and its main components are mucopolysaccharides, proteins and inorganic salts. Mucopolysaccharide is an acidic heteropolymer polysaccharide formed by the alternating combination of hexosamine and hexuronic acid. The main component in cartilage is chondroitin sulfate. Chondroitin sulfate and collagen are also important components of fish cartilage matrix. Th...

Claims

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Application Information

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IPC IPC(8): A61K35/60A61P29/00A61P19/02
CPCA61K35/60A61P19/02A61P29/00
Inventor 邢晓丹
Owner 浦江县昂宝生物技术有限公司
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