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Method for extracting activated sludge extracellular polymers

A technology of extracellular polymers and activated sludge, applied in sludge treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve problems such as low yield, incomplete coverage, and no unified extraction steps , to achieve the effect of high purity and high extraction efficiency

Inactive Publication Date: 2019-11-15
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting extracellular polymers from activated sludge, which aims to solve the problem that although the existing EPS extraction methods have many types, there is no unified extraction step, and there are low yields, incomplete types, Technical issues such as extraction process that may alter or destroy EPS composition

Method used

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  • Method for extracting activated sludge extracellular polymers
  • Method for extracting activated sludge extracellular polymers
  • Method for extracting activated sludge extracellular polymers

Examples

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Embodiment 1

[0084] A method for extracting LB-EPS of activated sludge extracellular polymers. The resuspension of the first sludge sediment is heated at 60°C or 80°C in a water bath for 5 minutes, and then the centrifugal force is 13000×g, the temperature Under the condition of 4°C, centrifuge for 15 minutes to separate the LB-EPS supernatant and the second sludge sediment.

Embodiment 2

[0089] A method for extracting ionic TB-EPS in the second sludge sediment, comprising: according to the Na 2 S·9H 2 The mass ratio of O to the second sludge sediment is 1:4, and the Na 2 S·9H 2 O was added to the resuspension of the second sludge sediment, and after being treated for 1 hour under airtight conditions with a rotation speed of 400 rpm, a temperature of 4°C, and full of nitrogen and protected from light, the NH 4 The mass ratio of F to the second sludge sediment is 1:5.9, adding the NH 4 F, under the conditions of rotation speed of 600 rpm and temperature of 4°C, the treatment was carried out for 1 hour, and the ionic TB-EPS supernatant and the third sludge sediment were separated.

Embodiment 3

[0094] A method for extracting hydrophobic TB-EPS in the third sludge sediment, comprising: adding SDS 140mg / g VSS to the third sludge sediment, stirring at 600rpm, 4°C for 45min, and then passing through 13000× g. Centrifuge at 4°C for 15 minutes, and then filter the supernatant through a 0.22 μm filter membrane, which is the hydrophobic TB-EPS.

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Abstract

The invention belongs to the technical field of sewage biology, and particularly relates to a method for extracting activated sludge extracellular polymers. The method comprises the following steps: pretreating activated sludge, carrying out soluble extracellular polymeric substance (S-EPS) extraction, carrying out loosely bound extracellular polymeric substance (LB-EPS) extraction, carrying out ionic tightly bound extracellular polymeric substance (TB-EPS) extraction, and carrying out hydrophobic tightly bound extracellular polymeric substance (TB-EPS) extraction. According to the method forextracting the activated sludge extracellular polymers, the liquid phase environment of EPS extraction is optimized, the combination of different extraction methods is screened, an inorganic ion extraction agent is selected, and different types of surfactants are screened to separately and sequentially extract four different types of EPS, namely S-EPS, LB-EPS, ionic TB-EPS and hydrophobic TB-EPS in the activated sludge, so that extraction efficiency of different types of EPS is high, purity is high, and the cell damage is small. The adopted inorganic ionic extraction agents are small in changedegree of the extracellular polymer, are green and environmentally friendly, and are accurate in quantification.

Description

technical field [0001] The invention belongs to the technical field of sewage biology, in particular to a method for extracting activated sludge extracellular polymers. Background technique [0002] Microbial aggregates widely exist in some natural and artificial aquatic systems. In the field of sewage treatment, the existence of microbial aggregates can not only improve the efficiency of mud-water separation and ensure good effluent, but also endow a micro-ecosystem that free bacteria cannot provide, enabling many different types of bacteria to be in their suitable ecological niches, and then Removal of different types of pollutants. Extracellular Polymeric Substances (EPS) is a kind of polymeric polymer compound produced by microbial cells under specific environmental conditions, and its components include proteins, polysaccharides, humic substances, nucleic acids, lipids and uronic acids an organic macromolecule. EPS accounts for 85% to 90% of the total organic carbon ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/127C02F11/13C02F11/121C08B37/00
CPCC02F11/121C02F11/127C02F11/13C08B37/0003
Inventor 安强赵彬陈宇航彭咏雪张鹏
Owner CHONGQING UNIV
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