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Immobilization method of candida antarctica lipase B

A technology of Candida Antarctica and lipase, applied in the field of enzyme engineering, can solve problems such as loss, and achieve the effects of convenient operation, good temperature stability and solvent tolerance, and simple preparation conditions

Active Publication Date: 2019-10-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although MOFs can improve many properties of enzymes, MOFs itself is a powdery substance, which is easily absorbed by materials during the experimental operation and causes loss.

Method used

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  • Immobilization method of candida antarctica lipase B
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  • Immobilization method of candida antarctica lipase B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Immobilization conditions of lipase B of Candida antarctica and determination method of lipase activity

[0024] Dimethylimidazole and zinc nitrate were added into deionized water, respectively, and sonicated at 40 Hz for 20 minutes to prepare 0.3125mol / L dimethylimidazole aqueous solution and 0.3125mol / L zinc nitrate aqueous solution. Take 60mL 0.3125mol / L dimethylimidazole aqueous solution, 1.5mL 0.3125mol / L zinc nitrate aqueous solution and 3mL Candida antarctica lipase B enzyme solution (Denmark Novozymes CALB, enzyme amount 72mg / ml, 4166.67 U / ml) was mixed to obtain a mixed enzyme solution, and the reaction was magnetically stirred at 25° C. and 200 rpm for 30 min to obtain a reaction solution containing MOFs (CALB-ZIF-8) complex. Add 0.5 g of macroporous resin D101-1 and 2.5 mL of 25% polyvinylimide (PEI) aqueous solution with a mass concentration of 25% by weight to the reaction solution, cross-linking reaction for 5 hours at 30°C and 200 rpm in a water ba...

Embodiment 2

[0031] Example 2 Effect of the ratio and concentration of solution used in the preparation process of immobilized enzyme on enzyme activity

[0032] The volume of the zinc nitrate aqueous solution in Example 1 was set to 1.0 mL-2.4 mL, and the other operations and conditions were the same as in Example 1. The results are shown in Table 1. The optimal volume ratio of the dimethylimidazole aqueous solution to the zinc nitrate aqueous solution is 40: 1.

[0033] Table 1: Results of specific enzyme activity of different volumes of zinc nitrate aqueous solutions

[0034]

Embodiment 3

[0035] Example 3 Effect of the amount of enzyme solution used in the preparation process of immobilized enzyme on enzyme activity

[0036] The volume of the Candida antarctica lipase B enzyme solution in Example 1 was set to 1-6 ml, and the other operations and conditions were the same as those in Example 1. The results are shown in Table 2. The optimal amount of enzyme solution added was 3 mL (72 mg / ml).

[0037] Table 2 Results of specific enzyme activity of different volumes of enzyme solution

[0038]

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Abstract

The invention discloses an immobilization method of candida antarctica lipase B. The method comprises the steps: mixing a dimethylimidazole aqueous solution, a zinc nitrate aqueous solution and Candida antarctica lipase B, carrying out reaction for 30 min under the magnetic stirring conditions of the temperature of 25 DEG C and the speed of 200 rpm, then adding non-polar macroporous adsorption resin and a crosslinking agent, carrying out cross-linking reaction for 5 h under the water bath conditions of the temperature of 15-45 DEG C and the speed of 200 rpm, carrying out suction filtration bya vacuum suction filter, drying a filter cake, and thus obtaining the immobilized candida antarctica lipase B. Compared with conventional commercial immobilized lipase on the market, the prepared immobilized lipase has higher enzyme activity, so the enzyme immobilization method has a good industrial application prospect.

Description

[0001] (1) Technical field [0002] The invention belongs to the field of enzyme engineering, and relates to a method for immobilizing Candida antarctica lipase B. [0003] (2) Background technology [0004] Lipase (Lipase, EC3.1.1.3, glyceride hydrolase) belongs to the class of carboxyl ester hydrolase, which can gradually hydrolyze triglycerides into glycerol and fatty acids, as well as the decomposition, esterification and esterification of ester compounds. Functions of exchange, lactone synthesis, peptide synthesis, ester polymerization and acylation reaction. Lipase exists in fat-containing animal, plant and microbial (such as mold, bacteria, etc.) tissues. Among them, Candida antarctica lipase B (CALB) is an important lipase. Candida antarctica lipase B is widely used in optically active drugs, structural lipids with special physiological functions, fine chemicals, chiral compounds, and biodiesel, and has great application value. [0005] Lipase-catalyzed reactions have the ch...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/08
CPCC12N9/20C12N11/08C12N11/14C12Y301/01003
Inventor 郑建永蔡鑫勇章银军汪钊
Owner ZHEJIANG UNIV OF TECH
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