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Preparation method of zearalenone ratio fluorescence probe

A technology of zearalenone and ratiometric fluorescent probes, which is applied in the fields of nano-optics, fluorescence/phosphorescence, and luminescent materials, can solve the problems of expensive instruments, low sensitivity, and complicated operations, and achieve high selectivity, high sensitivity, and The effect of simple preparation process

Active Publication Date: 2019-10-15
NANJING UNIV OF TECH
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Problems solved by technology

In recent years, thin-layer chromatography, gas chromatography, high-performance liquid chromatography, liquid chromatography-mass chromatography, and enzyme-linked immunoassay have been developed successively. These methods often have the disadvantages of low sensitivity, complicated operation, or expensive instruments.

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  • Preparation method of zearalenone ratio fluorescence probe
  • Preparation method of zearalenone ratio fluorescence probe
  • Preparation method of zearalenone ratio fluorescence probe

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specific Embodiment 1

[0022] Specific embodiment 1, the preparation of zearalenone fluorescent probe comprises the following steps:

[0023] (1) Preparation of silicon quantum dots: Add 0.3180g of sodium citrate to 8mL of glycerin, pass through argon protection, stir for 20min, then add 3-aminopropyltriethoxysilane (APTES) (as silicon source ), continue to stir for 10 min, then transfer to an oil bath, and raise the temperature to 185° C. to continue the reaction for 1.5 h. The solution changed from transparent and colorless to yellow, and had bright blue fluorescence under the irradiation of ultraviolet light, indicating the formation of silicon quantum dots. The crude silicon dots cooled to room temperature were transferred to a dialysis bag with a molecular weight cut-off of 3500 Da (MWCO: 3500), dialyzed for 24 hours with ultrapure water as the dialysate, and the solution in the dialysis bag was collected and lyophilized for storage.

[0024] (2) Preparation of cadmium telluride quantum dots: ...

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Abstract

The invention discloses a preparation method of a zearalenone ratio fluorescence probe. The method comprises the following steps: (1) preparation of a silicon quantum dot: APTES((3-aminopropyl)-triethoxysilane) and sodium citrate are subjected to a reaction in glycerol, and silicon quantum dots are obtained; (2) preparation of CdTe (cadmium telluride) quantum dots: tellurium powder, sodium borohydride and the like are subjected to a reaction, a NaHTe (sodium hydrogen telluride) precursor is obtained and added to the reaction solution, and red CdTe quantum dots are obtained; (3) preparation ofaptamer grafted silicon quantum dots: the prepared silicon quantum dots, N-(3-dimethylaminopropyl)-N-carbodiimide hydrochloride, N-hydroxysuccinimide, and a zearalenone aptamer are subjected to a reaction in an HEPES buffer solution, and silicon fluorescence quantum dots with surface grafted with the zearalenone aptamer are obtained; (4) synthesis of the ratio fluorescence probe: the red CdTe quantum dots are mixed with the silicon fluorescence quantum dots with surface grafted with the zearalenone aptamer, and the fluorescence probe is obtained. The fluorescence probe is high in selectivity and sensitivity, simple to prepare and low in cost.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to the preparation and application of a zearalenone ratio fluorescent probe. Background technique [0002] Zearalenone is a mycotoxin secreted by Fusarium fungus and widely contaminating cereal crops. It has estrogen interference, liver, immunity, and genotoxicity. Zearalenone pollution not only greatly endangers the development of agriculture and animal husbandry, but also causes serious food safety problems. Therefore, establishing a simple, rapid, sensitive and cheap detection method for zearalenone is of great significance for controlling the toxin content in grains and agricultural by-products. Important practical application and social significance. In recent years, thin-layer chromatography, gas chromatography, high-performance liquid chromatography, liquid chromatography-mass chromatography, and enzyme-linked immunoassay have been developed successively. These ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/06G01N21/64B82Y20/00B82Y30/00B82Y40/00
CPCC09K11/883C09K11/06G01N21/6428B82Y20/00B82Y30/00B82Y40/00C09K2211/10C09K2211/1088
Inventor 杨雅琼谭新柳黄菲张立慧石子莹刘兴华明灯明黄和
Owner NANJING UNIV OF TECH
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