Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for culturing pathogenic bacteria of gummy stem blight of sweet potatoes

A technology for culture medium and pathogenic bacteria, applied in the field of microorganisms, can solve the problems of few conidia, difficulty in completing pathogenicity determination and drug screening test, restricting the application of pathogenic bacteria of vine blight, etc., so as to promote vegetative growth and reproductive growth, The effect of promoting the formation of

Inactive Publication Date: 2019-09-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many mediums for cultivating fungi, such as PDA medium, oat medium, corn flour medium, carrot medium, etc., but these mediums cannot meet the requirements of rapid growth of the pathogenic bacteria of sweet potato blight, which has become The bottleneck in the research of this bacterium makes it difficult to complete the pathogenicity assay and drug screening test of this bacterium
In addition, it is difficult to induce the conidia of the fungus Blight blight. Although some specific media can meet the requirements of rapid growth of mycelia, there are few conidia produced, which seriously restricts the pathogenic bacteria Blight blight. Application in related research of sweet potato breeding and pathology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium for culturing pathogenic bacteria of gummy stem blight of sweet potatoes
  • Culture medium for culturing pathogenic bacteria of gummy stem blight of sweet potatoes
  • Culture medium for culturing pathogenic bacteria of gummy stem blight of sweet potatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the influence of the culture medium of different carbon source, nitrogen source on the growth of sweet potato blight pathogenic bacteria

[0033] In this example, on the basis of Chapei's medium, the effects of different carbon and nitrogen sources on the pathogen of sweet potato blight were studied by changing the carbon and nitrogen sources.

[0034] The components of Cha's medium are: 1 g of dipotassium hydrogen phosphate, 0.5 g of potassium chloride, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 O) 0.5g, ferrous sulfate is 0.01g, potassium nitrate 3g, sucrose 30g, agar is 20g.

[0035] Maltose, xylose, fructose, lactose, mannose, soluble starch, dextrin, sorbitol, glucose, galactose, trehalose, ribose, cellulose, and rhamnose were used to replace sucrose in Chapei's medium, keeping The nitrogen source in each culture medium was potassium nitrate, and 14 kinds of culture media with different carbon sources were prepared.

[0036] L-cysteine, glycine,...

Embodiment 2

[0054] Embodiment 2: the influence of different mediums on the growth of sweet potato blight pathogenic bacteria

[0055] (1) The preparation of Cha's medium with maltose as the sugar source and peptone as the nitrogen source and the cultivation of the pathogenic bacteria of the blight

[0056] Step 1: Take by weighing dipotassium hydrogen phosphate 1g, potassium chloride 0.5g, magnesium sulfate heptahydrate (MgSO 4 ·7H 2 (0) 0.5g, ferrous sulfate is 0.01g, peptone 3g, maltose 30g, agar are 20g;

[0057] Step 2: Mix the weighed medicines together, add deionized water to make the volume to 1000mL, and adjust to pH=7;

[0058] Step 3: Place the prepared medium in an autoclave, autoclave at 121°C for 30 minutes, and then take it out for use;

[0059] Step 4: Pour the sterilized culture medium into the plate in a sterile environment for standby;

[0060] Step 5: In a sterile environment, use sterilized tweezers to pick up the sweet potato blight pathogenic bacteria cake with...

Embodiment 3

[0092] Embodiment 3: the impact of culture conditions on the spore production of sweet potato blight pathogenic bacteria

[0093] According to the experimental results of Example 2, it is found that dark culture can not induce sporulation of the pathogenic bacterium of the blight, although the growth rate of the pathogen can be improved through the optimization of the medium, it cannot effectively promote the sporulation. Therefore, the present invention further improves the sporulation of the blight The culture conditions of the pathogenic bacteria were optimized in order to obtain a method that can promote the spore production of the pathogenic bacteria of Blight blight.

[0094] In this example, several different culture media were taken as examples to study the mycelium growth and spore production under different culture conditions.

[0095] A: The culture medium with optimized carbon source and nitrogen source, the medium components are: dipotassium hydrogen phosphate 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a culture medium for culturing pathogenic bacteria of gummy stem blight of sweet potatoes. The culture medium comprises a carbon source, a nitrogen source, dipotassium hydrogenphosphate, magnesium sulfate heptahydrate, potassium chloride, ferrous sulfate, agar and water, wherein the carbon source is maltose, galactose or ribose, and the nitrogen source is peptone or L-proline. Due to the fact that the culture medium contains the specific carbon source and the specific nitrogen source which are suitable for growth of the pathogenic bacteria of the gummy stem blight, thegrowth speed and the biomass of the pathogenic bacteria of the gummy stem blight are greatly improved, the bacterial colony diameter can reach 80 mm or above after 7 days of culture, and powerful support is provided for the morphological structure, biological characteristics, medicament control and screening and the like of the pathogenic bacteria in the next biological experiment.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a culture medium for cultivating the pathogenic bacteria of variegated blight and a cultivation method for promoting the spore production of the pathogenic bacterium of variegated blight. Background technique [0002] Sweet potato (Dioscorea esculenta (Lour.) Burkill) named sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc., is a twining herbaceous vine of Convolvulaceae Dioscorea. Native to tropical America, it was introduced into China at the end of the 16th century, and there are currently more than 500 species. Sweet potatoes are distributed from south to north and from east to west in China, and are widely planted in Sichuan, Henan, Shandong, Guangzhou, Anhui, Zhejiang and other places. my country's sweet potato planting area accounts for about 70% of the world's total. More than 100 million tons, accounting for 80% of the world's total output. Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 谢昀烨王汉荣方丽王连平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com