Preparation method of carrier of supported AHL-QS degrading enzyme
A technology of AHL-QS and degrading enzymes, applied in chemical instruments and methods, sustainable biological treatment, water/sludge/sewage treatment, etc., can solve problems such as poor stability, easy inactivation, and easy loss, and achieve Large pore volume, easy operation and low cost
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[0025] A preparation method for a carrier loaded with AHL-QS signal molecule degrading enzyme, comprising the steps of:
[0026] (1) UiO-66 loaded with AHL-QS signal molecule degrading enzyme: UiO-66 was synthesized by solvothermal method, UiO-66 was sieved into 30-35 mesh, and then ultrasonically activated to load AHL-QS degrading enzyme , the loading method is the adsorption method, the AHL-QS signal molecule degrading enzyme and UiO-66 are mixed in a phosphate buffer at a mass ratio of 0.75:1,
[0027] pH = 6.5-7.5, and then put it into a shaker with a temperature of 30-35°C and a rotation speed of 100-200r / min for 5-6h of adsorption.
[0028] The phosphate buffer is a mixed solution of trisodium phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride and ultrapure water in a mass ratio of 0.01:0.06:0.07:0.001:1.
[0029] The AHL-QS signal molecule degrading enzymes are all enzymes including acyltransferase and lactonase that have a degrading effect ...
Embodiment 1
[0033] (1) UiO-66 loaded with AHL-QS signal molecule degrading enzyme: UiO-66 was synthesized by solvothermal method, UiO-66 was sieved into 35 meshes, and then ultrasonically activated to load AHL-QS degrading enzyme, loaded The method is the adsorption method. AHL-QS signal molecule degrading enzyme and UiO-66 are mixed in a phosphate buffer at a mass ratio of 1:1, pH=7.5. Then, the temperature is set to 35°C and the rotation speed is 200r / Adsorption was carried out in a shaker for 6 hours.
[0034] (2) Preparation of AHL-QS signal molecule degrading enzyme carrier: select the density, size and pore size as 800-1000kg / m respectively 3 , 15×15×15mm and 50PPI polyurethane sponge, use small scissors to cut out an appropriate gap in the polyurethane sponge, use a small medicine spoon to place UiO-66 adsorbed with AHL-QS degrading enzyme inside the sponge, and then insert the sponge into In the hollow spherical plastic carrier with a diameter of 10mm, the gap cut out by the pol...
Embodiment 2
[0036] (1) UiO-66 loaded with AHL-QS signal molecule degrading enzyme: UiO-66 was synthesized by solvothermal method, UiO-66 was sieved into 30 meshes, and then ultrasonically activated to load AHL-QS degrading enzyme, loaded The method is the adsorption method. AHL-QS signal molecule degrading enzyme and UiO-66 are mixed in a phosphate buffer at a mass ratio of 0.75:1, pH=7, and then the temperature is set to 30°C and the rotation speed is 150r / Adsorption was carried out in a shaker for 6 hours.
[0037] (2) Preparation of AHL-QS signal molecule degrading enzyme carrier: select the density, size and pore size as 800-1000kg / m respectively 3 , 15×15×15mm and 50PPI polyurethane sponge, use small scissors to cut out an appropriate gap in the polyurethane sponge, use a small medicine spoon to place UiO-66 adsorbed with AHL-QS degrading enzyme inside the sponge, and then insert the sponge into In the hollow spherical plastic carrier with a diameter of 10mm, the gap cut out by the...
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