Breeding method of cordyceps militaris high-activity strain

Abstract

The invention belongs to the technical field of cordyceps militaris cultivation, and discloses a breeding method of a cordyceps militaris high-activity strain. A cordyceps militaris monospore strain is separated artificially, and gene mating types of cordyceps militaris are distinguished by adopting a molecular biology method; monospore strains of active substances such as high-yield cordycepin and adenosine are screened; through screening of nutritional compatibility, a pair of strains of different gene mating types are selected and paired, and further, a high-yield target strain is obtainedthrough cordyceps militaris outlet verification. The breeding method of the cordyceps militaris high-activity strain has the advantages that a traditional strain breeding technology by changing a culture medium formula and simple mating type pairing is changed, pairing and cordyceps militaris outlet verification are carried out, the technology bottleneck of no quantized investigation parameters inbreeding work is broken through, and the breeding method can be applied to rejuvenation of cordyceps militaris degradation strains, can also be applied to breeding of high-activity strains, gets ridsof the blindness and randomness of a traditional breeding mode, and has important significance for stabilizing the yield of cordyceps militaris in industrial production, inhibiting strain degeneration, rejuvenating the degraded strains and breeding the high-activity strains.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps militaris cultivation, and in particular relates to a method for breeding high-activity strains of Cordyceps militaris. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Cordyceps militaris, also known as Northern Cordyceps militaris, is a fungus that can parasitize on the pupae and larvae of Lepidoptera insects. It belongs to the same genus of Ergotaceae Cordyceps as Cordyceps sinensis, and has similar active ingredients to Cordyceps sinensis It is a traditional nourishing Chinese medicine with a long history of application. Traditional Chinese medicine believes that it enters the two meridians of the lung and kidney, which can not only nourish the lung yin, but also nourish the kidney yang. "Compendium of Xinhua Materia Medica" records that Cordyceps militaris "whole plant: sweet in taste, flat in nature, beneficial to...

AI Technical Summary

Problems solved by technology

[0007] (1) In the prior art, the Cordyceps militaris strain causes the fruiting body output to reduce rapidly and strain preservation difficulty because of multiple passages

It did not take into account that mat-alpha and mat-HMG genotype strains could be separated from the original strain of Cordyceps militaris, and after re-pairing, the problem of strain degradation caused by high number of passages and long storage time could be solved;

[0008] (2) Some scholars expect to seek a certain ratio between the mating strains of different genes to obtain the high yield of fruiting bodies of Cordyceps militaris, but they have not considered the optimal inoculation quality ratio of different Cordyceps militaris strains mat-alpha and mat-HMG strains is different;

[0009] (3) The nutritional incompatibility of monospore strains has not been considered in the existing breeding method of Cordyceps militaris based on genetic mating type. If the nutritional incompatibility between strains will lead to the incompatibility of nutrients between strains, the failure to pass The fruiting body of Cordyceps militaris grows during the sexual reproduction stage

[0010] (4) In the prior art, some scholars began to obtain monospore strains by isolation, and selected the optimal ratio between the two mating types, expecting to obtain high yield of Cordyceps militaris, but ignored the content of active substances in the fruiting bodies of Cordyceps militaris , the ability of different monospore strains to metabolize and produce active substances such as Cordyceps militaris polysaccharides and adenosine is different

[0013] The present invention separates single spores from the conidia of Cordyceps militaris, obtains two single spore strains of different mating types, and analyzes the yield of the active substance of the strain and the nutrient affinity between the strains, and selects the nutrient affinity and the activity in the mycelium. The mat-alpha and mat-HMG strains with high content of components are paired to prepare liquid seeds as the cultivar of Cordyceps militaris. This method has not been reported at home and abroad.

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