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Slimming and fat reducing composition and application thereof

A technology of composition and extract, applied in the field of biochemistry, can solve problems such as combined use that has not yet been reported, and achieve the effects of reducing body weight, preventing hyperlipidemia and coronary heart disease

Active Publication Date: 2019-08-16
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although both berberine hydrochloride and the fat-soluble extract of Ascidian brevista have certain blood lipid-lowering activities, the combination of the two has not been reported in the existing studies

Method used

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  • Slimming and fat reducing composition and application thereof
  • Slimming and fat reducing composition and application thereof
  • Slimming and fat reducing composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] In the present invention, the preparation method of the fat-soluble extract of Brachydia zodiacum comprises the following steps:

[0027] (1) Homogenize the Brachydia zoster, filter to obtain the filtrate;

[0028] (2) Extract the filtrate with n-butanol, separate the n-butanol part, concentrate to extract, and obtain the fat-soluble extract of Ascidian breviputum.

[0029] In the invention, the Brachydia zoster is homogenized and filtered to obtain a filtrate. In the present invention, before the homogenization, the impurities and attachments of the Ascidian squirrel are preferably removed. Preferably in the present invention, the Brachydia zoster is crushed and then mixed with physiological saline for homogenization. In the present invention, the mass ratio of the Brachygnathus constellata to physiological saline is preferably 1:3-1:5, more preferably 1:4. In the present invention, the homogenate is preferably an ice-bath homogenate, and the ice-bath homogenate is ...

Embodiment 1

[0038] 1. Preparation of the extract of Brachydontida zodiac

[0039] Remove the impurities and attachments of Brachyptina zodiac, cut into small pieces with surgical scissors, add normal saline according to the ratio of 1:4 volume ratio of Brachydia zodiac: normal saline, and homogenize in ice bath for 3 times, and change the homogenate each time. A new ice-water bath was used to avoid the change of active substances caused by the increase of temperature. The slurry was filtered with a Buchner funnel and the filtrate was collected. The filtrate was extracted with n-butanol (volume ratio 1:3), repeated 3 times, the extracted n-butanol fractions were combined, concentrated under reduced pressure at 45°C to an extract, and the lipid-soluble extract of Brachydia zoster was obtained for future use.

[0040] 2. Composition Preparation

[0041] The fat-soluble extract of Brachydia zodiac is mixed with commercially available berberine hydrochloride (purity ≥ 90%) at a weight ratio ...

Embodiment 2

[0042] Example 2 Composition Weight Loss and Lipid-lowering Activity

[0043] 1 main instrument

[0044] Fully automatic microplate reader (TECAN); carbon dioxide incubator (Thermo); constant temperature incubator (China, Harbin); microscope (Japan, OLYMPUS); Junyi); horizontal shaker (Beijing, Dingguo); chemiluminescence imaging system (DNR), etc.

[0045] 2 main reagents

[0046] Serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL) detection kit (purchased from Nanjing Jiancheng Institute of Biology); prolactin (Prolactin) ELISA detection reagent Box (purchased from Wuhan Uersen); Ham's F12k medium (USA, Gibco); fetal bovine serum (Israel, BI); horse serum (MRC); adenosine A1 receptor antibody (Beijing Boaosen); GAPDH antibody ; horseradish peroxidase-labeled goat anti-rabbit IgG (Bioworld); R-PIA (Sigma); DPCPX (Sigma);

[0047] 3 test drugs

[0048] The fat-reducing composition for weight loss prepared in Examp...

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Abstract

The invention provides a slimming and fat reducing composition and application thereof, and relates to the field of biochemical technology. The slimming and fat reducing composition, comprises, by weight, 6-15 parts of berberine hydrochloride and 1-5 parts of aplidium constellatum fat-soluble extract. The aplidium constellatum fat-soluble extract is obtained by homogenizing aplidium constellatum,performing filtering to obtain filtrate, extracting the filtrate by n-butanol and concentrating the extract. The composition has the remarkable effects of reducing body weight and regulating blood lipids, and can obviously prevent and treat hyperlipidemia, prevent obesity, reduce the body weight, regulate lipoprotein abnormality and lipid metabolism, and reduce the incidence of coronary heart disease.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a composition for weight loss and fat reduction and application thereof. Background technique [0002] Berberine hydrochloride is an isoquinoline alkaloid. Molecular formula [C 20 h 18 NO 4 ] + . Also known as berberine. It exists in many plants of 4 families and 10 genera such as Berberidaceae. Berberine can precipitate yellow needle-like crystals from ether. The melting point is 145°C. Soluble in water, insoluble in benzene, ether and chloroform. Chinese patent CN1939310A discloses a weight loss composition composed of berberine and oryzanol, which discloses that berberine has the effect of inhibiting adipogenesis, and inhibits the differentiation of preadipocytes by inhibiting the gene expression of PPARγ2; berberine hydrochloride (Berberine hydrochloride) has low solubility and is not easy to be absorbed by the human body, while oryzanol is a mixture of ferulic...

Claims

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Application Information

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IPC IPC(8): A61K35/655A61P3/04A61P3/06A61P9/10A61P1/16A23L33/00A61K31/4375
CPCA23V2002/00A61K31/4375A61K35/655A23L33/00A61P1/16A61P3/04A61P3/06A61P9/10A61K2300/00A23V2200/3324A23V2200/326A23V2200/3262A23V2200/30A23V2250/2042
Inventor 王雪艳唐旭东
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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