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Application of cryptotanshinone in preparation of chemosensitizer for Ph+ acute lymphocytic leukemia

A technology of acute lymphocytes and cryptotanshinone, applied in the field of biomedicine, can solve problems such as recurrence, organ damage, and obstruction of normal body cell division

Active Publication Date: 2019-06-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also big problems in the application of the above-mentioned therapies: First, although the commonly used chemotherapy regimens based on cell cycle blockers can prevent the abnormal proliferation of leukemia cells, they also seriously hinder the division of normal body cells; , the metabolic process of chemotherapy drugs is complicated, and the patient's heart, liver, kidney and other important organs are severely damaged; thirdly, although commonly used chemotherapy can quickly alleviate the disease, it is very easy to produce drug resistance and relapse

Method used

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  • Application of cryptotanshinone in preparation of chemosensitizer for Ph+ acute lymphocytic leukemia
  • Application of cryptotanshinone in preparation of chemosensitizer for Ph+ acute lymphocytic leukemia
  • Application of cryptotanshinone in preparation of chemosensitizer for Ph+ acute lymphocytic leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1 The study of combined administration of cryptotanshinone and imatinib in Ph+ acute lymphoblastic leukemia cell lines

[0085] A certain concentration gradient of cryptotanshinone and imatinib were used to treat Ph+ acute lymphoblastic leukemia cell line Sup-B15 cells for 24 hours, while the concentration of cryptotanshinone was fixed, and different gradient concentrations of imatinib were used to treat Sup-B15 cells for 24 hours. , the MTT colorimetric method was used to calculate the respective growth inhibition intensity of cells under five different drug treatment modes.

[0086] The specific research methods are as follows:

[0087] 1. Experimental cell lines

[0088] Sup-B15Ph+ acute lymphoblastic leukemia cell line was purchased from ATCC cell bank in the United States.

[0089] 2. Cell culture:

[0090] Sup-B15 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine cell culture at a constant ...

Embodiment 2

[0110] Example 2 The study of combined administration of cryptotanshinone and triptolide in Ph+ acute lymphoblastic leukemia cell lines

[0111] A certain concentration gradient of cryptotanshinone and triptolide were used to treat Ph+ acute lymphoblastic leukemia cell line Sup-B15 for 24 hours, while the concentration of anticancer drug triptolide was fixed, and cryptotanshinone with different gradient concentrations was used to treat Sup-B15. After 24 hours of cells, the MTT colorimetric method was used to calculate the respective growth inhibition strengths of the cells under four different drug treatment modes.

[0112] The specific research methods are as follows:

[0113] 1. Experimental cell lines

[0114] Sup-B15Ph+ acute lymphoblastic leukemia cell line was purchased from ATCC cell bank in the United States.

[0115] 2. Cell culture:

[0116] Sup-B15 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine ce...

Embodiment 3

[0137] Example 3 Study of Cryptotanshinone Combined with Artemisinin in Ph+ Acute Lymphoblastic Leukemia Cell Lines

[0138] A certain concentration gradient of cryptotanshinone and artemisinin were used to treat the Ph+ acute lymphoblastic leukemia cell line Sup-B15 for 24 hours, while the concentration of the anticancer drug cryptotanshinone was fixed, and different gradient concentrations of artemisinin were used to treat Sup-B15 cells for 24 hours. , the MTT colorimetric method was used to calculate the inhibitory intensity of cell proliferation under four different drug treatment modes.

[0139] The specific research methods are as follows:

[0140] 1. Experimental cell lines

[0141] Sup-B15Ph+ acute lymphoblastic leukemia cell line was purchased from ATCC cell bank in the United States.

[0142] 2. Cell culture:

[0143] Sup-B15 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine cell culture at a constant...

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Abstract

The invention discloses an application of cryptotanshinone in preparation of a chemosensitizer for acute lymphocytic leukemia, and application of combination of cryptotanshinone and anticancer drugs in preparation of drugs for treating and / or preventing acute lymphocytic leukemia, drug resistance reversal drugs and / or drugs for preventing relapse after pre-treatment. According to research discovery of the present invention, when cryptotanshinone and anticancer drugs are combined for use, cryptotanshinone and anticancer drugs are compounded to greatly increase the sensitivity of leukemia cellsto anticancer drugs, and the proliferation inhibition effect of multiple anticancer drugs on acute lymphocytic leukemia cells is improved in an explosive manner, so that the usage amount of each drugis far lower than that of the current clinical drug, and the explosive synergistic interaction effect is realized. Therefore, cryptotanshinone is used as an effective chemosensitizer for acute lymphocytic leukemia, and has important research significance and wide application prospect in the aspects of treating acute lymphocytic leukemia and solving the problem of drug resistance of acute lymphocytic leukemia.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically relates to the application of cryptotanshinone in the preparation of Ph+ acute lymphoblastic leukemia chemotherapy sensitization drugs. Background technique [0002] Leukemia, commonly known as "blood cancer", is a type of malignant blood tumor disease derived from hematopoietic stem cells, which is divided into lymphocytic leukemia and myeloid leukemia. Lymphocytic leukemia is divided into acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL). Among them, Ph+ acute lymphoblastic leukemia (Ph+ALL) has a rapid course of disease, a high degree of malignancy, and a very poor prognosis. Drugs targeting the Philadelphia chromosome (Philadelphia, Ph) - TKI inhibitors (such as imatinib), etc., have good curative effect when patients take them at the initial stage, but the curative effect is limited, and drug resistance will soon appear. [0003] In view of ...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61K45/06A61K31/506A61K31/585A61K31/366A61P35/02
CPCA23V2002/00A61K31/5025A61K31/506A61K31/58A61K45/06A23L33/105A61P35/02A61K2300/00A23V2250/2124A23V2200/308
Inventor 董博文周惠屈良鹄
Owner SUN YAT SEN UNIV
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