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Preparation method and applications of rice bran polysaccharide iron

A technology of rice bran polysaccharide and ferric chloride hexahydrate, which is applied in the field of polysaccharides, can solve the problems of large cell toxicity and achieve the effect of low cytotoxicity, low cytotoxicity and high bioavailability

Inactive Publication Date: 2019-05-31
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the non-viral vectors reported so far contain a large number of amino groups, resulting in the vector itself having greater cytotoxicity.

Method used

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  • Preparation method and applications of rice bran polysaccharide iron
  • Preparation method and applications of rice bran polysaccharide iron
  • Preparation method and applications of rice bran polysaccharide iron

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Take 10mL of 1% acetic acid solution, and configure it into rice bran polysaccharide acetic acid solution, and the mass concentration of rice bran polysaccharide in the solution is 0.6%. Then add 3g FeCl 3 ·6H 2 O crystals were ultrasonically treated for 10-15 minutes to dissolve them completely to obtain a casting solution.

[0055] Pour the film-casting liquid onto a clean and dry glass plate, let it cast naturally to form a film, and then dry it in an oven at 50°C to obtain rice bran polysaccharide-FeCl 3 Composite film.

[0056] The dried rice bran polysaccharide-FeCl 3 The composite membrane was soaked in 0.06mol / L NaOH solution and left to stand for 7 days, then taken out, washed with ethanol and acetone, and dried to obtain iron rice bran polysaccharide.

Embodiment 2

[0058] Take 10mL of 2% acetic acid solution, and configure it into rice bran polysaccharide acetic acid solution, and the mass concentration of rice bran polysaccharide in the solution is 0.8%. Then add 4g FeCl 3 ·6H 2 O crystals were ultrasonically treated for 10-15 minutes to dissolve them completely to obtain a casting solution.

[0059] Pour the film-casting liquid onto a clean and dry glass plate, let it cast naturally to form a film, and then dry it in an oven at 45°C to obtain rice bran polysaccharide-FeCl 3 Composite film.

[0060] The dried rice bran polysaccharide-FeCl 3 The composite film was soaked in 0.1mol / L NaOH solution and left to stand for 7 days, then taken out, washed with ethanol and acetone, and dried to obtain iron rice bran polysaccharide.

Embodiment 3

[0062] Take 10mL of 1% acetic acid solution, and configure it into rice bran polysaccharide acetic acid solution, and the mass concentration of rice bran polysaccharide in the solution is 0.5%. Then add 2g FeCl 3 ·6H 2 O crystals were ultrasonically treated for 10-15 minutes to dissolve them completely to obtain a casting solution.

[0063] Pour the film-casting liquid onto a clean and dry glass plate, let it cast naturally to form a film, and then dry it in an oven at 40°C to obtain rice bran polysaccharide-FeCl 3 Composite film.

[0064] The dried rice bran polysaccharide-FeCl 3 The composite film was soaked in 0.08mol / L NaOH solution and left to stand for 6 days, then taken out, washed with ethanol and acetone, and dried to obtain iron rice bran polysaccharide.

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Abstract

The invention discloses a preparation method and applications of rice bran polysaccharide iron, and belongs to the technical field of polysaccharide. The preparation method comprises following steps:rice bran polysaccharide is dissolved in an acetic acid solution so as to obtain a rice bran polysaccharide acetic acid solution; ferric trichloride hexahydrate crystals are dissolved in the rice branpolysaccharide acetic acid solution so as to obtain a film casting solution; the film casting solution is poured onto a glass plate for natural curtain coating film forming and drying so as to obtaina rice bran polysaccharide composite film; the rice bran polysaccharide composite film is immersed in an alkaline solution for standing, and washing and drying are carried out so as to obtain rice bran polysaccharide iron. The rice bran polysaccharide iron can be taken as an iron supplementation agent, or a gene carrier. The rice bran polysaccharide iron is prepared using the preparation method,and is used in preparation of iron supplementation agents and gene carriers effectively, so that defects of conventional iron supplementation agents such as low bioavailability, low absorption efficiency, and large side effect are avoided, and the defects of conventional non-virus gene carriers such as high cytotoxicity are avoided.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to a preparation method and application of rice bran polysaccharide iron. Background technique [0002] Iron is the trace element with the highest content in the human body. It participates in many physiological functions of the body, such as forming hemoglobin to participate in the transportation and storage of oxygen, forming myoglobin and improving the body's immunity. However, iron deficiency anemia is one of the most common diseases in the world, especially the elderly, children and women are more susceptible to iron deficiency due to their physiological specialties, and iron deficiency seriously affects people's physical fitness and intelligence. Iron supplements are effective drugs for the treatment and prevention of iron deficiency anemia. The most common iron supplements currently on the market are inorganic salts represented by ferrous sulfate. This type of iron s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/16C12N15/87
Inventor 刘梁潘显虎陈新廖荣熊学敏
Owner WUHAN POLYTECHNIC UNIVERSITY
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