Fodder for sows in lactation period and preparation method thereof
A technology for lactating and breeding sows, applied in the field of feed for sows during lactation and its preparation, can solve the problems of unbalanced nutritional content of blood meal, unbalanced mineral content, low energy content of chicken manure, etc., and increase the average daily Effects of feed intake, increased production efficiency, increased lactation and milk fat content
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Embodiment 1
[0022] Example 1 Strain Enzyme Production Ability Test
[0023] 1. Strain types: Mucor, Aspergillus niger, Trichoderma reesei.
[0024] 1. Medium:
[0025] Separation medium (%): KH 2 PO 4 0.036, MgSO 4 ·7H 2 O 0.05, ZnCl 2 0.0014, cheese 0.4, Na 2 HPO 4 ·7H 2 O 0.107, NaCl 0.016, CaCl 2 0.0002, FeSO 4 0.0002, peptone 0.005, agar 2.0. pH 7.0.
[0026] Shake flask fermentation medium (%): corn flour 4.0, bean cake powder 3.0, bran 2.5, Na 2 HPO 4 0.4, KH 2 PO 4 0.03. pH 7.0.
[0027] Solid fermentation medium: rice bran mixed with water.
[0028] 2. Test method
[0029] Experiment 1 group: According to the volume ratio of 1:1:1, inoculate the three strains into the shake flask fermentation medium at the same time, culture at 30°C and 190r / min for 3 days, then centrifuge the fermentation broth at 10000r / min for 25 minutes, and take the supernatant Enzyme activity measured by liquid; experiment group 2: according to the volume ratio of 1:1:1, Trichoderma ...
Embodiment 2
[0040] 1. Preparation of compound microbial seed solution
[0041] Use the comprehensive PDA slant medium to cultivate Aspergillus niger for 3 days, Mucor for 5 days, and Betide yeast extract for 6 days; respectively use 10.0mL sodium chloride injection and inoculation hook to scrape and wash the mycelia of the slant lawn for 3 times , transferred to the Erlenmeyer flask containing 20.0mL sodium chloride injection, stirred with a magnetic stirrer for 30 minutes, and prepared into 140,000 / mL single spore and single cell suspensions of 3 strains of bacteria respectively.
[0042] Take 10.0 mL of bacterial suspension in 100.0 mL of PDA corn stalk powder compound medium, and culture Aspergillus niger at 150 r / min at 30°C for 2 days, Mucor for 2 days, and Trichoderma reesei extract for 3 days to prepare three kinds of The seed liquid of the strain. According to the volume ratio of Mucor: Aspergillus niger: Trichoderma reesei=1:1:1, measure three kinds of seed liquids respectively,...
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