A kind of immune enhancer based on Mortierella sinensis and its preparation method
An immune enhancer, the technology of Trichospora sinensis, is applied in the field of immunopotentiator and preparation based on Trichospora sinensis to achieve the effects of improving human immunity, increasing the content of immune active substances and high economic benefits
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Embodiment 1
[0038] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:
[0039] 1) Strain selection
[0040] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );
[0041] 2) Strain culture
[0042] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:
[0043] 2.1) Preparation of primary solid bacteria
[0044]The isolated and purified slant strains were inoculated on the solid medium of a 9 cm petri dish, the medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 Add 3.0 g of agar, 20.0 g of agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in an incubator at 17°C for 20 days to obtain a first-grade solid strain.
[0045] ) secondary liquid seed culture
[0046] Put 200ml of liquid medium in a 500mL conical flask; each 1...
Embodiment 2
[0055] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:
[0056] 1) Strain selection
[0057] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );
[0058] 2) Strain culture
[0059] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:
[0060] 2.1) Preparation of primary solid bacteria
[0061] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish. The medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust pH to 6.5 with 1.0 M NaOH; put in 20 ° C incubator, culture time is 30 days, and obtain first-class solid strains.
[0062] ) secondary liquid shake flask seed culture
[0063] Put 200ml of liquid medium in a 500mL Erlenmeyer flask, containin...
Embodiment 3
[0073] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:
[0074] 1) Strain selection
[0075] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );
[0076] 2) Strain culture
[0077] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:
[0078] 2.1) Preparation of primary solid bacteria
[0079] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish (the medium was: 200.0 g of potato juice, 20.0 g of glucose, 10.0 g of maltose, 10.0 g of peptone, 10.0 g of yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g of agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0M NaOH), put them in an incubator at 18.5°C, and cultivate for 25 days to obtain a first-grade solid strain.
[0080] ) secondary liquid shake flask seed culture
[0081] Put 200ml of liquid medium in a...
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