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Method for determining content of hesperidin in dried tangerine or orange peel by virtue of ASE-HPLC (accelerated solvent extraction-high performance liquid chromatography) process

A technology of hesperidin and tangerine peel, applied in the field of chemical detection, can solve the problems of long reflux time and high professional level requirements of technicians

Inactive Publication Date: 2017-08-04
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] This method takes a long time to reflow and requires a high level of expertise for technicians

Method used

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  • Method for determining content of hesperidin in dried tangerine or orange peel by virtue of ASE-HPLC (accelerated solvent extraction-high performance liquid chromatography) process
  • Method for determining content of hesperidin in dried tangerine or orange peel by virtue of ASE-HPLC (accelerated solvent extraction-high performance liquid chromatography) process
  • Method for determining content of hesperidin in dried tangerine or orange peel by virtue of ASE-HPLC (accelerated solvent extraction-high performance liquid chromatography) process

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Instruments and reagents

[0030] 1.1 Instruments:

[0031] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), Agilent1290 liquid chromatograph

[0032] 1.2 Reagent: Water: Class I water in accordance with GB / T 6682;

[0033] Methanol (CH 4 O): Chromatographically pure (for liquid chromatography).

[0034] 2. Method

[0035] 2.1 Preparation of reference substance solution: Take an appropriate amount of hesperidin reference substance, weigh it accurately, add methanol to make a solution containing 0.4mg per 1ml, and obtain it.

[0036] 2.2 Preparation of the test solution

[0037] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for the test product:

[0038] Take about 1g of the coarse powder of this product, weigh it accurately, put it in a Soxhlet extractor, add 80ml of petroleum ether (60-90℃), heat and reflux for 2-3 hours, discard the petroleum ether, evaporate the dregs to dryness, and add 80ml of met...

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Abstract

The invention discloses a method for determining content of hesperidin in dried tangerine or orange peel by virtue of an ASE-HPLC (accelerated solvent extraction-high performance liquid chromatography) process and belongs to the field of chemical detection. The method is short in extraction time and high in detection accuracy. The method disclosed by the invention adopts an ASE process for extracting dried tangerine or orange peel powder, and collecting methanol extract liquor; and determining the content of hesperidin in the methanol extract liquor by adopting an HPLC process. The method disclosed by the invention can replace a pharmacopeia method for extracting the hesperidin in the dried tangerine or orange peel and determining the content of hesperidin.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for determining the content of hesperidin in tangerine peel. Background technique [0002] "Chinese Pharmacopoeia" 2015 Edition Part One Preparation method of the test product: Take about 1g of the coarse powder of this product, weigh it accurately, put it in a Soxhlet extractor, add 80ml of petroleum ether (60-90°C), and heat to reflux for 2-3 hours , discard the petroleum ether, volatilize the dregs, add 80ml of methanol, heat and reflux until the extract is colorless, let it cool, filter, put the filtrate in a 100ml measuring bottle, and fractionate it with a small amount of methanol [0003] Wash the container once, filter the lotion into the same measuring bottle, add methanol to the mark, shake well, and you get it. [0004] This method takes a long time to reflux and requires a high professional level of technicians. Contents of the invention [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 廖强陈学松韦日伟王丽丽欧妮
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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