Extraction condition optimization method and extraction method of tripterygium multi-index active ingredients
A technology of active ingredients and extraction conditions, which is applied in the field of extraction conditions optimization and extraction of multi-index active ingredients of Tripterygium To achieve the effect of simple extraction, fewer experiments, saving resources and energy consumption
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Embodiment 1
[0045] A method for optimizing extraction conditions of tripterygium wilfordii multi-index active ingredients
[0046] 1. Extraction experiment
[0047] Weigh the root powder of tripterygium wilfordii with a balance, extract with a mixed solution of one or more components in methanol, ethyl acetate, and acetonitrile, and use a liquid-to-solid ratio of 1:1 to 80:1 between the extraction solution and the powder, Ultrasonic extraction at 50-400W power for 5-60 minutes; solid-liquid separation to obtain an extract containing multiple-index active ingredients of Tripterygium wilfordii. After the extract was filtered through the membrane, it was used for liquid chromatography tandem mass spectrometry detection (R.J.Zhuo, H.B.Xiao et al. Rapid simultaneous quantitative determination of terpenoids in Tripterygium wilfordii Hook F by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry . Analytical Methods, 2013, 5, 2046–2052). Utilizin...
Embodiment 2
[0078] Take the powder of Tripterygium wilfordii medicinal root, add ethyl acetate methanol solution with ethyl acetate volume concentration of 75% according to the liquid-to-material ratio of 32, ultrasonically extract for 30 minutes under 250W ultrasonic power, and separate solid and liquid to obtain Tripterygium wilfordii multi-index Extract of the active ingredient. After the extract was filtered through the membrane, it was used for detection by liquid chromatography tandem mass spectrometry. The calculated OD value is 6.509, and the extraction rate reaches 97.31%.
Embodiment 3
[0080] Take the powder of Tripterygium wilfordii medicinal root, add ethyl acetate methanol solution with a volume concentration of 75% ethyl acetate according to the liquid-to-material ratio of 40, ultrasonically extract for 30 minutes under 250W ultrasonic power, and separate solid and liquid to obtain Tripterygium wilfordii multi-index Extract of the active ingredient. After the extract was filtered through the membrane, it was used for detection by liquid chromatography tandem mass spectrometry. The calculated OD value is 6.513, and the extraction rate reaches 97.37%.
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