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A method of using 5-aminolevulinic acid to improve sunflower's resistance to parasitic weeds Helianthus helianthus

A technology of aminolevulinic acid and sunflower, applied in the direction of botany equipment and methods, application, seed and rhizome treatment, etc., to achieve the effect of reducing the use and protecting the ecological environment

Active Publication Date: 2021-03-23
ZHEJIANG SCI-TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The invention provides a method for improving the resistance of sunflowers to the parasitic weed Helianthus helianthus by using 5-aminolevulinic acid, effectively alleviating the problem that sunflower helianthus parasitism affects the growth of sunflowers in the sunflower planting process, and will greatly reduce the use of herbicides, Protect the environment

Method used

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  • A method of using 5-aminolevulinic acid to improve sunflower's resistance to parasitic weeds Helianthus helianthus
  • A method of using 5-aminolevulinic acid to improve sunflower's resistance to parasitic weeds Helianthus helianthus
  • A method of using 5-aminolevulinic acid to improve sunflower's resistance to parasitic weeds Helianthus helianthus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1 Preparation of different concentrations of 5-aminolevulinic acid

[0039] Weigh 5-aminolevulinic acid in the dark, and prepare 0, 5, 10, 15, 20 mg / L ALA mediation solution with distilled water.

[0040] Table 1 Concentration gradient of 5-aminolevulinic acid

[0041]

[0042] Note: The recommended concentration refers to the optimal concentration obtained after passing the test, that is, the ALA content added per 1L of water.

[0043] 2 Materials and methods

[0044] 2.1 Pretreatment and germination of sunflower seeds

[0045] Select plump and healthy sunflower TK0409 seeds to peel off the exocarp, sterilize in 70% absolute ethanol solution for 2 minutes, rinse with sterile water several times, put the seeds in different concentrations of ALA solution, soak for 18 hours; fully absorb the ALA solution The seeds were washed twice with distilled water, put into a petri dish with two layers of filter paper containing distilled water, and cultured at 25° C. in the da...

Embodiment 2

[0056] Example 2 Determination of antioxidant enzymes

[0057] Antioxidant enzyme activity includes ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and glutathione reductase (GR) , the antioxidant enzyme activity was measured with a Muti-ModeReader (SYNERGY HTX) instrument according to the following method.

[0058] 1. Determination of APX enzyme

[0059] The APX activity assay method was improved according to the method of Nakano and Asada (1981). The reaction system was 3 mL, including 2.9 mL of 100 mM potassium phosphate buffer (pH 7.0, containing 0.1 mM EDTA-Na 2 , 0.3mM Ascorbic Acid, 0.06mM H 2 o 2 ) and 100 μl enzyme extract. Measure the absorbance change of the reaction mixture within 1min at 290 nm, and the extinction coefficient is 2.8mM -1 cm -1 .

[0060] 2. Determination of CAT enzyme

[0061] CAT activity through H 2 o 2 Determination of consumption (the extinction coefficient is 39.4mM -1 cm -1 ). The react...

Embodiment 3

[0074] The measurement of embodiment 3 MDA and ROS

[0075] MDA and ROS were measured with a spectrophotometer (UV-5500, METASH).

[0076] 1. Determination of MDA

[0077] The content of MDA was determined according to Zhou and Leul (1999) with modified thiobarbituric acid colorimetric method. Add 5 mL of 0.5% thiobarbituric acid (TBA, prepared with 10% trichloroacetic acid) solution to 2 mL of the supernatant enzyme solution, react in a water bath at 95 ° C for 30 min, and immediately put it in an ice bath; Colorimetric at the place, the difference is used to calculate the MDA content, the extinction coefficient is 155mM -1 cm -1 .

[0078] 2. Determination of ROS

[0079] Reactive oxygen species (ROS) including hydrogen peroxide (H 2 o 2 ), superoxide anion (O 2 - ),Hydroxyl radicals( - OH).

[0080] 2.1H 2 o 2 Determination of

[0081] h 2 o 2 According to the method of Velikova et al. (2000), the reaction system was 2 mL, containing 0.5 mL of supernatant, 0...

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Abstract

The invention provides an application of 5-aminolevulinic acid to improvement of resistance of sunflowers to parasitic weed sunflower orobanche Cumana and further finds that 5-aminolevulinic acid improves the resistance of the sunflowers to orobanche Cumana by promoting the synthesis of an antioxidase in sunflower plants, reducing the levels of MDA and ROS in the sunflower plants and increasing the concentration of GSH in the sunflower plants and the GSH / GSSG ratio. The problem that the growth of the sunflowers is affected by parasitism of sunflower orobanche Cumana in a sunflower planting process is effectively relieved, the usage amount of a herbicide is greatly reduced, and the ecological environment is protected.

Description

technical field [0001] The invention relates to the technical field of crop planting, in particular to a method for improving sunflower resistance to parasitic weeds Helianthus helianthus by using 5-aminolevulinic acid. Background technique [0002] Sunflower is an important economic crop in the world and one of the five major oil crops in my country. In recent decades, environmental changes have brought many biotic and abiotic stress problems to crop production, especially the infestation of weeds has caused great harm to crop yield and quality, so weed control is of great importance to agricultural production and the environment. are critical and will play a major role in determining whether we can meet future food production needs. [0003] Sunflower Liedang (Orobanche cumana Wallr.) is a non-photosynthetic root parasitic weed that mainly parasitizes the roots of sunflowers and connects to the host plant’s tissue system through a special structure called haustoria, deple...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01G13/00A01G22/00
Inventor 许玲李娟娟曹梦婷周伟军杨翀朱金文王尖白全江
Owner ZHEJIANG SCI-TECH UNIV
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