Mycoplasma hyopneumoniae subunit vaccine and preparation method and application thereof

Abstract

The invention provides a mycoplasma hyopneumoniae subunit vaccine and a preparation method and application thereof. A plurality of antigenic proteins of mycoplasma are connected by a flexible Linker and expressed as a chimeric protein, and the proteins are mutually unaffected, and can retain respective immunogenicity. The mucosal immune protection effect of the vaccine is greatly enhanced by adding the escherichia coli heat-labile enterotoxin B subunit and the dendritic cell-inducing peptide to the chimeric protein. The invention also relates to a preparation method of a mycoplasma hyopneumoniae chimeric antigen and application of the mycoplasma hyopneumoniae chimeric antigen to the subunit vaccine for preventing mycoplasma hyopneumoniae infection. The mycoplasma chimeric antigen is expressed in bacillus megaterium and can be used for industrial preparation of safe mycoplasma hyopneumoniae subunit vaccines.

Description

technical field [0001] The invention belongs to the field of veterinary vaccines, and in particular relates to a subunit vaccine of mycoplasma hyopneumoniae and a preparation method and application thereof. Background technique [0002] Mycoplasma hyopneumoniae (Mhp) can cause Mycoplasma hyopneumoniae pneumonia, also known as endemic pneumonia, which is a chronic consumptive respiratory disease of pigs with a high clinical infection rate. Its main symptoms are cough and wheezing, and it is characterized by high morbidity and low mortality. Once the pigs are infected with Mhp, it is difficult to remove it, which not only seriously affects the growth and development of the pigs, increases the dosage of drugs, but also is prone to secondary infection of PRRSV, PCV, etc., resulting in the failure of various vaccinations. The incidence rate of mycoplasma swine pneumonia in my country is 50% to 80%, and the mortality rate in newly infected areas is 5% to 15% (mostly suckling pigs...

AI Technical Summary

Problems solved by technology

[0003] Since Mycoplasma hyopneumoniae has very strict requirements on the nutritional conditions of the culture medium, it is difficult to grow in general culture medium, and it is a kind of difficult culture in animal mycoplasma, and the culture period is long, and it is very easy to contaminate Mycoplasma hyorhina during the culture process. Mycoplasma flocculus, etc., make the production cost of Mycoplasma pneumoniae whole-bacteria inactivated vaccine remain high

Although the protein expressed by the baculovirus expression system has a post-translational modification system, the use of baculovirus to express foreign genes in insect cells can carry out post-translational modifications, correct protein folding and glycosylation, and the expression of eukaryotic gene products It can better phosphorylate, amidate, cut signal peptides, and form tertiary or quaternary structures, but it has disadvantages such as high production costs, many steps, long cycle, and difficult control of production quality.

Although the Escherichia coli expression system is simple and easy to operate, there are problems such as endotoxin contamination and inability to secrete and express

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