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Human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry

A technology of human immunoglobulin and immunoglobulin, applied in the direction of biological testing, measuring devices, material inspection products, etc., to achieve the effects of easy promotion, cost saving, and reduction of manual dilution and other steps

Inactive Publication Date: 2019-04-16
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of how to provide a detection kit with wide detection linear range, high detection accuracy and good detection stability

Method used

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  • Human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry
  • Human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry
  • Human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry

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preparation example Construction

[0037] In the preparation of the above kit, the preparation of the R1 reagent and the calibrator can be prepared according to the needs, and there is no special limitation. The preparation method of the R2 reagent includes the following steps:

[0038] Step 1. Add latex microspheres, buffer and antibody to the reagent bottle, place on a mixer and mix for more than 60 minutes, centrifuge at 12000rmp / min for 20 minutes, and discard the supernatant;

[0039] Step 2. Add buffer containing activator to the reagent bottle from which the supernatant was discarded in step 1, centrifuge at 12000rmp / min for 20min, and discard the supernatant;

[0040] Step 3. Place the reagent bottle obtained in step 2 on a mixer and mix for 60 minutes, add buffer, protective agent and preservative, and ultrasonicate. The ultrasonic conditions are: power 500W, ultrasonic 5s, interval 5s, ultrasonic time 20min in total, Obtain R2 reagent;

[0041]It should be noted that the amount of each raw material i...

Embodiment 1

[0044] Human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetric method, including R1 reagent, R2 reagent and calibrator;

[0045] R1 reagent, consisting of 25mM Tris-HCl, 1% NaCl and 0.1% Proclin 300, pH 7.5±0.05 (25±1°C);

[0046] R2 reagent consists of 0.4% BSA, 0.1% Proclin 300, 0.2% antibody-coupled latex microspheres (the mass ratio of antibody to latex microspheres is 1:10) and 25mM HEPES buffer, the pH is 7.5±0.05 (25± 1°C);

[0047] The calibrator consists of multiple solutions, each solution includes human immunoglobulin G, 20mM PBS and 0.1% Procline300, the concentrations of human immunoglobulin G are 0mg / L, 6.25mg / L, 12.5mg / L, 37.5mg / L, 75mg / L and 150mg / L.

[0048] For the preparation of the above-mentioned human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetric method, the R1 reagent and calibrator can be configured according to the needs, and the preparation method of the R2 reagent is as follows:

[...

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Abstract

The invention, which belongs to the technical field of medical in-vitro diagnostics, relates to a human urine immunoglobulin G detection kit based on latex-enhanced immunoturbidimetry, thereby solvinga problem of providing a human urine immunoglobulin G content detection kit having advantages of wide detection linear range, high detection accuracy, and high detection stability. The kit comprisesan R1 reagent, an R2 reagent and a calibrator. The R1 reagent includes a buffer solution, NaCl and a preservative. The R2 reagent includes a buffer solution, an antibody-coupling latex microsphere, aprotective agent and a preservative; and the antibody is the goat anti-human immunoglobulin polyclonal antibody or a rabbit anti-human immunoglobulin polyclonal antibody. The calibrator is formed by aplurality of solution; each solution includes human immunoglobulin G, a buffer solution and a preservative; and the concentrations of the human immunoglobulin G in different solutions are different.The kit has advantages of wide detection linear range, high detection accuracy, and high detection stability.

Description

technical field [0001] The invention belongs to the technical field of medical in vitro diagnosis, and in particular relates to a human urine immunoglobulin G detection kit based on latex-enhanced immune turbidimetry. Background technique [0002] Immunoglobulin (Ig) refers to a globulin having antibody (Ab) activity or a chemical structure similar to an antibody molecule. Immunoglobulin G is the main immunoglobulin in the blood, most of which exist in the form of monomers, mainly composed of spleen and lymph, and cannot be filtered by glomeruli, so the content in human urine is extremely low. [0003] When the damage to the glomerular filtration membrane is relatively mild, the urinary microalbumin (MA) and transferrin (TRF) of medium molecular weight in the urine mainly increase in filtration. Aggravated, IgG began to appear in the urine. When the damage to the glomerular filtration membrane was severe, in addition to IgG being filtered out in the urine, IgM with a larger...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 张瑞夏冬梅孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
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