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A sample pretreatment method based on on-site detection of ion mobility spectrometry

A technology of ion mobility spectrometry and sample pretreatment, which is applied in the field of on-site detection, can solve the problems of fragile extraction head, expensive extraction needle, and limited promotion, and achieve the effect of low cost, convenient method, and improved detection efficiency

Active Publication Date: 2020-07-24
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, solid-phase microextraction technology is widely used in the removal and enrichment of on-site samples, but the commercial extraction needles of solid-phase microextraction are expensive, and the extraction head is fragile and easy to break, which limits the promotion of this technology.

Method used

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  • A sample pretreatment method based on on-site detection of ion mobility spectrometry
  • A sample pretreatment method based on on-site detection of ion mobility spectrometry
  • A sample pretreatment method based on on-site detection of ion mobility spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Seal the tip of the pipette with a parafilm, and add an appropriate amount of a mixture of styrene, n-hexane and azobisisobutyronitrile to the tip with a mass ratio of 5:2:1. The volume is 30ul, put it in an oven for in-situ polymerization, the oven temperature is 65°C, and a polymer monolithic column with extraction ability is obtained at the pipette tip, and the pipette tip prepared by the tip polymer is stored in a pipette tip box ;

[0025] (2) Extract the samples to be tested on site. Insert the pipette gun into the above-mentioned treated tip, dissolve the prohibited substance lorazepam in sleep-improving health care products in water (1ppm), take out 5ml of the sample liquid into a 10ml disposable centrifuge tube, and insert the tip of the tip into the test solution" Draw-out" after 50 cycles. Put 100ul of the eluate into a 2ml disposable centrifuge tube, and use a pipette to carry out the "suction-extrusion" cycle for 20 times, and the eluate contains the ...

Embodiment 2

[0028] (1) Seal the tip of the pipette with a parafilm, add an appropriate amount of a mixture of divinylbenzene, n-decyl alcohol and azobisisobutyronitrile to the tip, the mass ratio is 3:1:1, and the added mixture The total volume of the liquid is 3ul, put it into an oven for in-situ polymerization, the oven temperature is 75°C, and a polymer monolithic column with extraction ability is obtained at the pipette tip, and the pipette tip prepared by the tip polymer is placed in the pipette tip box Save in;

[0029] (2) Extract the samples to be tested on site. Insert the pipette gun into the above-mentioned treated tip, dissolve the psychoactive substance marijuana in water (1ppm), take out 10ml of the sample liquid into a 10ml disposable centrifuge tube, and insert the tip of the tip into the test solution to "suck-out" to carry out After 30 cycles. Put 20ul of the eluate into a 2ml disposable centrifuge tube, and use a pipette to carry out the "suction-push" cycle for 15 ti...

Embodiment 3

[0032] (1) Seal the tip of the pipette with a parafilm, add a mixture of divinylbenzene, styrene, toluene and azobisisobutyronitrile to the tip, the mass ratio is 10:3:2, and the added mixture The total volume of the liquid is 10ul, put it into an oven for in-situ polymerization reaction, the oven temperature is 80°C, a polymer monolithic column with extraction ability is obtained at the pipette tip, and the pipette tip prepared by the tip polymer is placed in the pipette tip box Save in;

[0033] (2) Extract the samples to be tested on site. Insert the pipette gun into the above-mentioned treated tip, dissolve the psychoactive substance ketamine in soda water (1ppm), take out 8ml of the sample liquid into a 10ml disposable centrifuge tube, and insert the tip of the tip into the test solution to "suck-out" "After 40 cycles. Put 500ul of the eluate into a 2ml disposable centrifuge tube, and use a pipette to carry out the "suction-push" cycle 20 times, and the eluate contains ...

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Abstract

The invention discloses a sample pretreatment method of on-site test based on ion migration spectrometry. The method comprises the following steps that A, a mixed solution is added at a gun head of apipette, is put into a drying oven to carry out in-situ polymerization reaction, and a polymer monolithic column with an extraction capability is obtained at the gun head; B, an on-site sample to be tested is extracted, the pipette is inserted into the treated gun head, after the gun head stretches into a test solution to carry out "suck in-push out" for multiple times in a circulation mode, a small amount of eluant is placed into a 2 ml disposable centrifugal pipe, and is carried out the "suck in-push out" for multiple times in a circulation mode, and the eluent contains the enriched sample;and C, ion migration spectrometry detects the enriched sample. According to the method, the problems that the trace of a compound to be detected and the water sample cannot be directly detected by theion migration spectrometry, a rapid and simple sample pretreatment method is provided, and the sample to be detected is detected on site; and the method can be applied to the fields of on-site screening of drug addictives, food illegal addition and the like.

Description

technical field [0001] The invention relates to the field of on-site detection, in particular to a sample pretreatment method for on-site detection based on ion mobility spectrometry. Background technique [0002] In recent years, the media has exposed a lot of drug abuse incidents in KTV, bars and other entertainment places, and in terms of food safety, such as soups, hot pots, drinks and some health products, it is reported that they contain some illegally added drugs. The improper use of these prohibited drugs will cause great damage to people's health, and the abuse of mentally prohibited drugs may also induce other illegal and criminal activities. At present, for the detection of these prohibited substances, some laboratory detection techniques are usually used, such as liquid chromatography-mass spectrometry (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS) to detect samples. For law enforcement personnel, it is necessary to judge whether there are contraband...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34G01N1/40G01N27/62
CPCG01N1/34G01N1/405G01N27/622
Inventor 杜振霞孙堂强
Owner BEIJING UNIV OF CHEM TECH
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