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Solid state fermentation medium for paecilomyces lilacinus and method for culturing paecilomyces lilacinus

A technology of Paecilomyces lilacinus and solid fermentation, which is applied in the field of cultivation of Paecilomyces lilacinus, can solve the problems of long fermentation time, high cost, low yield and the like, and achieves the effects of wide sources, low cost and pollution reduction.

Pending Publication Date: 2019-02-22
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For more than half a century, researchers at home and abroad have conducted extensive and in-depth research on Paecilomyces lilacinus, and achieved a series of research results in biology, ecology, pest control efficiency and field application. It shows great potential in prevention and control, and the market prospect is broad. Although the traditional fungal fermentation method can realize industrial production, there are problems such as long fermentation time, high cost, and low yield.

Method used

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  • Solid state fermentation medium for paecilomyces lilacinus and method for culturing paecilomyces lilacinus
  • Solid state fermentation medium for paecilomyces lilacinus and method for culturing paecilomyces lilacinus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Activation medium: PDA medium

[0045] Primary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water

[0046] Secondary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .

[0047] Solid fermentation medium for Paecilomyces lilacinus:

[0048] 20 servings of wheat bran, 5 servings of husk, 5 servings of corn cob, 20 servings of wood ear mushroom residue, 20 servings of eryngii mushroom residue, and 50 servings of apple pomace.

[0049] step:

[0050] Strain activation:

[0051] Configure PDA medium, restore the strain from the preservation state to room temperature, thaw the frozen strain, inoculate the preserved strain into the PDA medium for cultivation, select the strong colonies of the medium, and select some of the col...

Embodiment 2

[0062] Activation medium: PDA medium

[0063] Primary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .

[0064] Secondary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .

[0065] Solid fermentation medium for Paecilomyces lilacinus:

[0066] 15 servings of wheat bran, 4 servings of chaff, 6 servings of corn cob, 40 servings of fungus residue, 25 servings of apple pomace, and 10 servings of pear pomace.

[0067] step:

[0068] Strain activation:

[0069] Configure PDA medium, restore the strain from the preservation state to room temperature, thaw the frozen strain, inoculate the preserved strain into the PDA medium for cultivation, select the strong colonies of the medium, and select some of the colonies to inoculate t...

Embodiment 3

[0080] Activation medium: PDA medium

[0081] Primary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .

[0082] Secondary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .

[0083] Solid fermentation medium for Paecilomyces lilacinus:

[0084] 20 parts of wheat bran, 8 parts of husk, 5 parts of corn cob, 45 parts of Pleurotus eryngii dregs, 30 parts of apple dregs, 20 parts of pear dregs.

[0085] step:

[0086] Strain activation:

[0087] Configure PDA medium, restore the strain from the preservation state to room temperature, thaw the frozen strain, inoculate the preserved strain into the PDA medium for cultivation, select the strong colonies of the medium, and select some of the colonies to inoculate the new Culture ...

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Abstract

The invention provides a solid state fermentation medium for paecilomyces lilacinus and a method for culturing the paecilomyces lilacinus and belongs to the technical field of microbial culture. The solid state fermentation medium comprises the following components in parts by weight: 5-40 parts of wheat bran, 1-10 parts of rice husk, 1-10 parts of corncobs, 10-70 parts of mushroom residues and 10-70 parts of fruit residues. The method comprises the following steps: 1) inoculating activated paecilomyces lilacinus into a primary liquid medium for performing primary culture so as to obtain a primary seed solution; 2) inoculating the primary seed solution into a secondary liquid medium for performing secondary culture; and 3) inoculating the secondary seed solution into the solid state fermentation medium for performing solid fermentation culture for 5-8 days. When the solid state fermentation medium provided by the invention is utilized for culturing the paecilomyces lilacinus, the fermentation time can be shortened, the fermentation cost is reduced, and the yield of the paecilomyces lilacinus is increased.

Description

Technical field [0001] The invention belongs to the technical field of microbial culture, and particularly relates to a solid fermentation medium of Paecilomyces lilacinus and a method for culturing Paecilomyces lilacinus. Background technique [0002] Nematodes can invade and parasitize plants. The invaded and parasitic plants affect normal growth and development due to the absorption of nutrients by the nematodes, and the secretions of the nematode metabolism can stimulate the cells and tissues of the host plant, causing plant malformations. Most plant nematodes infest the underground parts of plants, such as roots, tubers, etc. The roots can show nodules, necrosis, stubby roots and clumps, resulting in a large reduction in crop yield or a serious decline in quality. Common plant nematode diseases include root knot nematode disease, soybean cyst nematode disease, wheat grain nematode disease, sweet potato stem nematode disease, rice stem nematode disease, and citrus semi-perfor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14
Inventor 陈晓燕李肖宇揭云东刘玉珍朱春苗黄娟翟修彩钱潘攀骆毛喜林克明余劲聪
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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