Solid state fermentation medium for paecilomyces lilacinus and method for culturing paecilomyces lilacinus
A technology of Paecilomyces lilacinus and solid fermentation, which is applied in the field of cultivation of Paecilomyces lilacinus, can solve the problems of long fermentation time, high cost, low yield and the like, and achieves the effects of wide sources, low cost and pollution reduction.
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Embodiment 1
[0044] Activation medium: PDA medium
[0045] Primary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water
[0046] Secondary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .
[0047] Solid fermentation medium for Paecilomyces lilacinus:
[0048] 20 servings of wheat bran, 5 servings of husk, 5 servings of corn cob, 20 servings of wood ear mushroom residue, 20 servings of eryngii mushroom residue, and 50 servings of apple pomace.
[0049] step:
[0050] Strain activation:
[0051] Configure PDA medium, restore the strain from the preservation state to room temperature, thaw the frozen strain, inoculate the preserved strain into the PDA medium for cultivation, select the strong colonies of the medium, and select some of the col...
Embodiment 2
[0062] Activation medium: PDA medium
[0063] Primary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .
[0064] Secondary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .
[0065] Solid fermentation medium for Paecilomyces lilacinus:
[0066] 15 servings of wheat bran, 4 servings of chaff, 6 servings of corn cob, 40 servings of fungus residue, 25 servings of apple pomace, and 10 servings of pear pomace.
[0067] step:
[0068] Strain activation:
[0069] Configure PDA medium, restore the strain from the preservation state to room temperature, thaw the frozen strain, inoculate the preserved strain into the PDA medium for cultivation, select the strong colonies of the medium, and select some of the colonies to inoculate t...
Embodiment 3
[0080] Activation medium: PDA medium
[0081] Primary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .
[0082] Secondary seed culture medium: sucrose 40g / L, soybean powder 20g / L, sodium nitrate 5g / L, dipotassium hydrogen phosphate 1.5g / L, zinc sulfate 0.23g / L, iron sulfate 0.05g / L, the balance is water .
[0083] Solid fermentation medium for Paecilomyces lilacinus:
[0084] 20 parts of wheat bran, 8 parts of husk, 5 parts of corn cob, 45 parts of Pleurotus eryngii dregs, 30 parts of apple dregs, 20 parts of pear dregs.
[0085] step:
[0086] Strain activation:
[0087] Configure PDA medium, restore the strain from the preservation state to room temperature, thaw the frozen strain, inoculate the preserved strain into the PDA medium for cultivation, select the strong colonies of the medium, and select some of the colonies to inoculate the new Culture ...
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