Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Photosensitizer and preparation method thereof

A photosensitizer and carrier technology, applied in the field of photosensitizers, can solve the problems of low luminous efficiency, shallow tissue penetration depth, etc., and achieve the effects of simple preparation process, strong absorption efficiency, and high-efficiency photodynamic therapy

Inactive Publication Date: 2019-01-22
SHENZHEN UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a photosensitizer and its preparation method to solve the problems of low upconversion luminescence efficiency and shallow tissue penetration depth of previous photosensitizers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Photosensitizer and preparation method thereof
  • Photosensitizer and preparation method thereof
  • Photosensitizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] The invention provides a method for preparing a photosensitizer, which is characterized in that it comprises the following steps:

[0043] S01: Heat 1 equivalent of gadolinium chloride, 15-50 equivalents of oleic acid, and 35-70 equivalents of octadecene in an inert gas environment; add 5-10 equivalents of NH after cooling 4 F and 1-2 times the equivalent of NaOH were reacted at 300°C for 1 hour, and NaGdF was obtained after precipitation treatment 4 Nanoparticles;

[0044] S02: 3 equivalents of yttrium trifluoroacetate, 1 equivalent of ytterbium trifluoroacetate, 0.1 equivalent of erbium trifluoroacetate, 50-100 equivalents of oleic acid, 30-60 equivalents of octadecene, and NaGdF4 in step S01 For nanoparticles, react in an inert gas environment at 280-320°C for 20-40min, and precipitate;

[0045] S03: 5 equivalents of yttrium trifluoroacetate, 1 equivalent of neodymium trifluoroacetate, 50-100 equivalents of oleic acid, 30-60 equivalents of octadecene, and NaGdF in ...

Embodiment 1

[0055] Upconversion Gd@Er@Nd@Lu(UP) multi-shell nanoparticles were prepared by thermal-co-precipitation method. ① First, prepare NaGdF 4 Nanoparticles, weighed gadolinium chloride GdCl 3 ·6H 2 O 0.3717g was added in the three-necked bottle, and 6ml oleic acid and 15ml octadecene were measured simultaneously in the three-necked bottle. Then, pass argon gas for 40 minutes to remove water vapor and oxygen in the reaction flask. Then heat up to 160°C, react for 30 minutes, cool down to room temperature, add NH 4 F (0.1480g) and NaOH (0.1000g), then heated to 100°C, after methanol was removed, the temperature was rapidly raised to 300°C, reacted for 1 hour, and finally lowered to room temperature, centrifuged with ethanol, and dispersed into n-hexane solution. ②Preparation of Gd@Er nanoparticles, first, weigh the corresponding amount of yttrium trifluoroacetate Y(CF 3 COO) 3 0.3336g, ytterbium trifluoroacetate Yb (CF 3 COO) 3 0.1024g, erbium trifluoroacetate Er (CF 3 COO...

Embodiment 2

[0057] Preparation of upconverting nanoparticles co-loaded with photosensitizers. First, the upconverting nanoparticles are phase-transferred. We used the amphiphilic polymer TWEEN20 to transfer the oily upconverting nanoparticles into the aqueous phase, then loaded the photosensitizer merocyanine (MC540) and chlorin (Ce6) molecules, stirred overnight, and then centrifuged to obtain the product UPMC and dispersed in PBS. Finally, polyethylene glycol-folate (DEPE-mPEG) was used to increase the biocompatibility of the nano-photosensitizer. Such as Figure 4 Shown, in the case of 50μg / mL cell viability was significantly reduced.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

An improved photosensitizer is prepared by using a multi-core-shell doped nanometer structure. The improved photosensitizer comprises an innermost layer composed of Gd ions, a first middle layer composed of Er ions, a second middle layer composed of Nd ions and an outer layer composed of Lu ions. An up-converting nanometer sphere is composed of all the layers and loads photosensitizer MC540 and Ce6 molecules. The photosensitizer has the advantages of a high penetration depth and high up-converting luminescent efficiency. The photosensitizer combined folic acid with biological compatibility isused for an internal photodynamic therapy well.

Description

technical field [0001] The invention relates to a photosensitizer, in particular to a nano particle up-conversion photosensitizer. Background technique [0002] Photodynamic therapy (PDT) is a function-preserving tumor ablation intervention. This disease treatment method was discovered at the beginning of the 20th century, and it was demonstrated for the first time by Dougherty et al. in 1975, and extensive research was carried out on PDT. PDT has become a special way of disease treatment. The most basic treatment includes: first treating the tumor site with a photosensitizer (PS), and then irradiating the tumor with light of a specific wavelength to activate the PS. The excited PS transfers its energy to molecular oxygen, thereby generating cytotoxic reactive oxygen species (ROS), such as singlet oxygen ( 1 o 2 ), etc., this oxygen can kill tumor cells. PDT itself has almost no toxic effects on biological systems. Compared with chemotherapy and radiotherapy, PDT will no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K41/00A61K47/02A61K47/22A61K47/60A61P35/00
CPCA61K9/5115A61K41/0057A61K41/0071A61K47/22A61K47/60A61P35/00A61K2300/00
Inventor 王丹薛彬宋军屈军乐
Owner SHENZHEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products