Method for extracting, purifying and inspecting selenomethionine in Astragali mushroom

Abstract

The invention discloses a method for extracting, purifying and inspecting selenomethionine in Astragali mushroom and relates to the field of extraction, purification and inspection of amino acid in Astragali mushroom. The method comprises the following steps: taking the Astragali mushroom as a raw material, adding a nitric acid solution of 5 percent with the material-liquid ratio being 1:6, centrifuging, adding Tris-Hcl into the precipitate, performing ultrasonic treatment, performing enzymolysis for 36 hours, centrifuging for 10 minutes, taking supernatant, performing ultrafiltration centrifugal suction filtration to obtain crude extracting liquid, taking n-butyl alcohol, acetic acid and water with the ratio of 7:1:2 as a developing agent, developing, separating and purifying the selenomethionine through thin layer chromatography, taking chromatographic methanol and ultrapure water as a flowing phase, measuring the molecular mass information of a sample by a mass spectrometer, performing infrared detection on a functional group, analyzing the molecule structure, measuring the content by a double-ultraviolet spectrophotometer, measuring the optical rotation and the specific rotation by a polarimeter, and measuring the melting point after the solution is evaporated and crystallized. According to the method, the low-content sensitive amino acid selenomethionine is not liable to damage, the extraction efficiency is high and the sensitivity is high; and the method is an extraction, purification and detection method which is simple to operate and high in efficiency.

Description

technical field [0001] The invention relates to the field of extraction, purification and inspection of amino acids in Beiqi mushroom, in particular to a method for extracting, purifying and inspecting selenomethionine in Beiqi mushroom. Background technique [0002] Beiqi mushroom, Pleurotus pleurotus under the Basidiomycota Agaricaceae, uses 74km of Hunyuan County, Shanxi Province 2 Due to the advantages of geographical resources in selenium-rich areas, Pleurotus ostreatus is a new type of edible fungus developed in Hunyuan selenium-rich areas in Shanxi. According to the bioaccumulation effect, Beiqi mushroom contains the pharmacological components of Hunyuan Astragalus, which can enrich astragaloside to a certain extent during the growth and development process. Beiqi mushroom contains trace element selenium and 8 amino acids necessary for human body. The content is relatively high, and only lysine is 1.185%, polysaccharide 9.688%, selenium 0.54ppm, zinc 77.77ppm, and co...

AI Technical Summary

Problems solved by technology

Currently, selenocystine and selenomethionine are identified by PC and TLC techniques, and selenomethionine is determined by hydrogen bromide GC method. When there are hydrocarbons, they are easily destroyed, the noise of the solvent has a great influence on the results, the operation of extraction and purification is cumbersome, and the results are unreliable, etc.

[0005] In order to solve the problem that the extraction, purification and inspection of selenomethionine in Beiqi mushroom are cumbersome, and the results are easily disturbed and unreliable, the present invention provides a kind of Beiqi mushroom that is easy to operate, has short test time, high extraction efficiency and is relatively mild. Method for extraction, purification and detection of selenomethionine in

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