A kind of preparation method of polypyrrole film substrate for cell culture

A technology of cell culture and polypyrrole, which is applied in the direction of cell culture support/coating, biochemical equipment and methods, tissue culture, etc., can solve the problems of limiting practical application, and achieve low material cost, simple equipment, and regular shape Effect

Active Publication Date: 2020-08-04
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many methods for the preparation of polypyrrole film substrates, but there are still unsolved problems that limit its practical application.

Method used

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  • A kind of preparation method of polypyrrole film substrate for cell culture
  • A kind of preparation method of polypyrrole film substrate for cell culture
  • A kind of preparation method of polypyrrole film substrate for cell culture

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Effect test

Embodiment 1

[0035] Such as figure 1 Shown, the present invention has prepared polypyrrole solution and polypyrrole film substrate by simple oxidative polymerization method and spin-coating method, comprises the following steps:

[0036] (1) Vacuum distillation: build a vacuum distillation device, connect the water inlet and outlet, turn on the oil pump, put the pyrrole reagent into the distillation bottle, stir it with a magnetic force and heat it at a constant temperature of 60°C, the distilled pyrrole monomer is colorless The oily liquid flows into the collection bottle through the condenser tube. After all the pyrrole monomers are purified, the oil pump is turned off, and the pyrrole monomers are collected in a clean test tube, sealed with a parafilm and stored in a 4°C refrigerator for later use.

[0037] (2) Synthesis of polypyrrole: take 0.068g of ammonium persulfate powder and dissolve it in 5ml of pure water to prepare aqueous solution of ammonium persulfate, weigh 0.08g of sodium...

Embodiment 2

[0042] Such as figure 1 Shown, the present invention has prepared polypyrrole solution and polypyrrole film substrate by simple oxidative polymerization method and spin-coating method, comprises the following steps:

[0043] (1) Vacuum distillation: build a vacuum distillation device, connect the water inlet and outlet, turn on the oil pump, put the pyrrole reagent into the distillation bottle, stir it magnetically and heat it at a constant temperature of 80°C, the distilled pyrrole monomer is colorless The oily liquid flows into the collection bottle through the condenser tube. After all the pyrrole monomers are purified, the oil pump is turned off, and the pyrrole monomers are collected in a clean test tube, sealed with a parafilm and stored in a 4°C refrigerator for later use.

[0044] (2) Synthesis of polypyrrole: take 0.034g of ammonium persulfate powder and dissolve it in 4ml of pure water to prepare an aqueous solution of ammonium persulfate, weigh 0.05g of sodium dodec...

Embodiment 3

[0049] Such as figure 1 Shown, the present invention has prepared polypyrrole solution and polypyrrole film substrate by simple oxidative polymerization method and spin-coating method, comprises the following steps:

[0050] (1) Vacuum distillation: build a vacuum distillation device, connect the water inlet and outlet, turn on the oil pump, put the pyrrole reagent into the distillation bottle, stir it magnetically and heat it at a constant temperature of 80°C, the distilled pyrrole monomer is colorless The oily liquid flows into the collection bottle through the condenser tube. After all the pyrrole monomers are purified, the oil pump is turned off, and the pyrrole monomers are collected in a clean test tube, sealed with a parafilm and stored in a 4°C refrigerator for later use.

[0051] (2) Synthesis of polypyrrole: take 0.136g of ammonium persulfate powder and dissolve it in 10ml of pure water to prepare an aqueous solution of ammonium persulfate, weigh 0.10g of sodium dode...

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Abstract

The invention relates to a method for preparing a polypyrrole film substrate for preparing a polypyrrole solution for cell culture, which is realized through the following technical scheme: building a vacuum distillation device to purify pyrrole monomers, using dimethyl sulfoxide as an organic solvent with pyrrole monomers, Catalyst and oxidant are reacted to obtain polypyrrole solution, polypyrrole film base is prepared by spin coating method, vacuum dried and sterilized for cell culture. The invention uses dimethyl sulfoxide as an organic solvent to increase the hydrophilicity of the polypyrrole film substrate and the ion permeability of the cell membrane. The spin coating method is easy to operate, and the thickness of the substrate can be adjusted by the number of times of spin coating. The prepared polypyrrole film substrate is safe, non-toxic, uniform in structure, has good electrical conductivity and stability, and the interaction with cells promotes the differentiation and growth of nerve cell synapses, which is beneficial to the transmission of electrical signals between cells and damaged nerves. Cellular repair.

Description

technical field [0001] The invention relates to a method for preparing a polypyrrole film substrate for preparing a polypyrrole solution for cell culture, and belongs to the technical field of nanomaterial preparation. Background technique [0002] Nanotechnology is one of the important contents of the scientific and technological industrial revolution in the 21st century. It is a highly interdisciplinary comprehensive discipline including physics, chemistry, biology, material science and electronics. It not only includes basic disciplines with observation, analysis and research as the main line, but also technical science with nano-engineering and processing as the main line. It is a complete system integrating cutting-edge science and high technology. The application of nanotechnology in the field of biomedicine has great potential, and a large number of basic research results indicate that it has great advantages in drug delivery, bioimaging and biosensing. As a branch o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/079C08J5/18C08L79/04C08G73/06
CPCC08G73/0611C08J5/18C08J2379/04C12N5/0618C12N2533/30
Inventor 王作斌杨雪马可王莹王馨悦曲英敏董莉彤宋正勋许红梅
Owner CHANGCHUN UNIV OF SCI & TECH
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