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Method for producing butanol by utilizing efficient fermentation of pichia pastoris solid waste

A Pichia pastoris and waste technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as cannot be simply discarded/discharged, and achieve the effect of saving consumption and improving economic performance

Active Publication Date: 2018-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to save costs, many factories often simply remove / refine the target product in the fermentation supernatant, and leave a large amount of semi-solid residues of Pichia pastoris, which cannot be simply discarded / discharged due to environmental safety reasons. simply store

Method used

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  • Method for producing butanol by utilizing efficient fermentation of pichia pastoris solid waste
  • Method for producing butanol by utilizing efficient fermentation of pichia pastoris solid waste
  • Method for producing butanol by utilizing efficient fermentation of pichia pastoris solid waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Embodiment 1: 7L static anaerobic fermenter fermentation

[0112] ABE fermentation was carried out in 15% corn flour medium on 7L tanks.

[0113] Fermentation 56h finishes, butanol concentration, ABE total concentration, total sugar utilization rate and total residual sugar concentration are respectively 9.78g / L, 15.72g / L, 43%, 55.00g / L (ABE fermentation performance see figure 1 ).

Embodiment 2

[0114] Embodiment 2: 7L static anaerobic fermenter fermentation

[0115] ABE fermentation was carried out on 7L tanks with 8% corn flour medium.

[0116] Fermentation 37h finishes, butanol concentration, ABE total concentration, total sugar utilization rate and total residual sugar concentration are respectively 4.79g / L, 7.75g / L, 45%, 17.60g / L (ABE fermentation performance see figure 2 , two For the changes of sugar / trisaccharide and total amino acid concentration, see Figure 5 ).

Embodiment 3

[0117] Embodiment 3: 7L static anaerobic fermenter fermentation

[0118] Carry out ABE fermentation with 8% corn flour medium on the 7L tank, the specification of waste Pichia treatment liquid is yeast content 400g / L, NaOH consumption 50g / L (after adjusting pH, SO 4 2- 58g / L), at the beginning of the solvent production period, after the pH bottomed out and rebounded to 0.1-0.2, 0.75L of the above-mentioned waste Pichia treatment solution was added, and the treatment solution was diluted 5 times, and the final total liquid volume was 3.75L. Cornmeal (actual) content, waste yeast content and SO in mixed stock 4 2- The contents were 6.4g / L, 80g / L, and 11.6g / L respectively (the average value was taken after 3 batches of fermentation using Pichia pastoris solid waste A).

[0119] Fermentation 44h finishes, butanol concentration, ABE total concentration, total sugar utilization rate and total residual sugar concentration are respectively 10.63g / L, 16.57g / L, 91%, 4.80g / L (ABE ferm...

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Abstract

The invention discloses a method for producing butanol by utilizing efficient fermentation of pichia pastoris solid waste, which belongs to the technical field of fermentation engineering and waste recycling. The method of the invention comprises the following steps: inoculating a corn powder culture medium with acetone clostridium butyri-cum to perform ABE fermentation, and when the fermentationreaches a solvent production period, adding pichia pastoris solid waste into the corn powder culture medium according to a given ratio to continue the fermentation. The method of the invention is usedfor producing the butanol, so that the recycling and reduction of the solid waste (pichia pastoris solid waste) can be realized, the fermentation raw materials (corn powder) can be saved, and the economical performance of the ABE fermentation can be improved.

Description

technical field [0001] The invention relates to a method for efficiently fermenting and producing butanol by using Pichia pastoris solid waste, and belongs to the technical field of fermentation engineering and waste recycling. Background technique [0002] Bio-butanol (Bio-butanol) is an important platform compound, which can be used to manufacture n-butyl plasticizers such as phthalic acid, aliphatic dibasic acid and phosphoric acid, as well as butyl acrylate and methacrylate, etc. Spray paint products are excellent solvents for the production of antibiotics, vitamins, and hormones. They are also important intermediates for the manufacture of chemical products such as butyraldehyde, butyric acid, butylamine, butyl lactate, glycol ether, and butyl acetate. A clean, renewable, high-efficiency liquid fuel and fuel additive. Therefore, it has very important applications in the fields of preparing chemical products, solvents, and high-efficiency liquid fuels / fuel additives. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12P7/36C12P7/06C12R1/145
CPCC12P7/06C12P7/16C12P7/36Y02E50/10
Inventor 史仲平丁健许萌谢方陈程
Owner JIANGNAN UNIV
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