Preparation method of fluorescent carbon dots from roasted chicken and method for detecting concentration of serum albumin by fluorescent carbon dot probe from roasted chicken

Abstract

The invention discloses a method for detecting concentration of serum albumin by a fluorescent carbon dot probe from roasted chicken. The method comprises the following steps: step 1, mixing fluorescent carbon dot solutions with different concentrations extracted from the roasted chicken with a human serum albumin solution to prepare standard solutions with different concentrations, detecting fluorescence intensity of each standard solution to obtain fluorescence spectrograms of the standard solutions, taking the difference between fluorescence intensity when the concentration of human serum albumin is zero and fluorescence intensity of the standard solutions with other concentrations as dependent variables, and establishing a linear relationship with the concentration of human serum albumin; step 2, randomly preparing a human serum protein sample solution containing fluorescent carbon dots, detecting concentration of serum albumin in the sample, and detecting concentration of serum albumin in the sample solution through the linear relationship. According to the method, the concentration of serum protein in the solution is detected through the linear relationship, the method has simple and convenient detection process, high sensitivity and low detection limit, and online in-situ rapid and sensitive detection of hemoglobin concentration of an actual sample can be realized.

Description

technical field [0001] The present invention relates to a method for detecting serum albumin concentration using fluorescent carbon dot probes from grilled chicken. Background technique [0002] In the prior art, serum albumin is the most abundant protein in plasma, and one of the most important carrier proteins in the body. It has important physiological functions such as binding and transport, maintaining osmotic pressure and pH, material exchange, and anticoagulation. Its complex chemical properties and a series of functional groups that allow multifunctional chemical derivatization, the current detection methods for human serum albumin include chemiluminescence, electrochemical, capillary electrophoresis and visible photometry, etc., these methods have pretreatment The method is cumbersome, the detection method is complicated, and the time is long. [0003] In the past ten years, with the rapid development of nanomaterials, fluorescent carbon dots are one of the most po...

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Problems solved by technology

[0002] In the prior art, serum albumin is the most abundant protein in plasma, and one of the most important carrier proteins in the body. It has important physiological functions such as binding and transport, maintaining osmotic pressure and pH, material exchange, and anticoagulation. Its complex chemical properties and a series of functional groups that allow multifunctional chemical derivatization, the current detection methods for human serum albumin include chemiluminescence, electrochemical, capillary electrophoresis and visible photometry, etc., these methods have pretreatment The method is cumbersome, the detection method is complicated, and the time is long, etc.

[0003] In the past ten years, with the rapid development of nanomaterials, fluorescent carbon dots are one of the most popular nanomaterials after titanium dioxide nanoparticles, carbon nanotubes, and graphene due to their stronger luminescent properties and higher stability. At present, the synthesis technologies of fluorescent carbon dots mainly include: electrolysis, high temperature pyrolysis, hydrothermal synthesis and chemical synthesis, etc. However, recent studies have found that carbon dots will be formed during thermal processing of grilled meat products that people eat daily. Through the above complex synthesis steps, using natural food as materials to prepare carbon nanoparticles is economical and environmentally friendly, and the inedible chicken scraps are thermally processed to prepare carbon dots. The price is low, and fluorescent carbon dots have high chemical stability, low toxicity and excellent performance. The unique advantages such as biocompatibility make it have good application prospects in the fields of biochemical sensing, drug carrier, imaging analysis, etc. At present, there is no research on fluorescent carbon dots extracted from natural food as serum albumin probes. to report

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