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Surface-modified bone meal and preparation method thereof

A surface modification and bone powder technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of bone cell adhesion and inductive reduction, and achieve the effects of good mechanical properties, tight adhesion, and normal growth shape.

Pending Publication Date: 2018-12-14
XIAN GIANT BIOGENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although calcined bone has great advantages as an alternative biomaterial, after degreasing, deproteinizing and high-temperature calcination, the organic components are completely removed, and the adhesion and induction to bone cells are greatly reduced.

Method used

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  • Surface-modified bone meal and preparation method thereof
  • Surface-modified bone meal and preparation method thereof
  • Surface-modified bone meal and preparation method thereof

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preparation example Construction

[0034] The preparation method of the involved surface modified bone meal specifically comprises the following steps:

[0035] Natural bovine cancellous bone is calcined and pulverized to obtain porous hydroxyapatite particles after pretreatment, with a particle size of 0.1-2.0 mm and a pore size of 50 nm-150 μm. The surface of the particles is modified by adsorbing medical biomacromolecules , to obtain surface-modified bone meal.

[0036] in:

[0037] The pretreatment of natural bovine cancellous bone is realized by the following steps:

[0038] Take the cancellous bone part of the bovine bone (metaphysis of femur of one-year-old bovine) and cut it into 2cm×2cm×2cm bone pieces. Bone marrow, cartilage and other tissues on the bone block are dry. After immersing the above bone fragments in NaOH solution with a concentration of 0.5 M for 6 h, 30% H 2 o 2 The solution was soaked for 6h, washed with ultrapure water and PBS (pH between 6.0-7.0), and dried. Most of the organic ...

Embodiment 1

[0048] Pre-treatment of calcined bones: select one-year-old bovine femur metaphysis, cut into 2 cm×2 cm×2 cm bone pieces, wash with ultrapure water, put them in a pot and boil them repeatedly for 8 times, during the boiling process Remove bone marrow, cartilage and other tissues on the bone block, and dry. After immersing the above bone in 0.5 M NaOH for 6 h, use 30% H 2 o 2 Soak for 6h, wash with ultrapure water and PBS (pH between 6.0-7.0), and dry. Most of the organic matter in the bone block was removed by the above steps.

Embodiment 2

[0050] Preparation of calcined bone powder and adsorption of medical biomacromolecules: put the prepared bovine bone into a muffle furnace for high-temperature calcination. The calcination process is calcination at 700°C for 4 hours, secondary calcination at 700°C for 3 hours, and washing with PBS until the pH value is 6.5-7.5, pulverize the bone particles with a particle size of 0.1-0.25 mm, place the bone particles in 20 mg / mL sodium hyaluronate solution for negative pressure adsorption for 2 hours, and dry at 37 °C.

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Abstract

The invention relates to surface-modified bone meal and a preparation method thereof. The preparation method comprises the following steps: pretreating natural bovine cancellous bone, then calcining and pulverizing to obtain porous hydroxyapatite particles, and modifying the surfaces of the particles by adsorbing medical biomacromolecules onto the surfaces of the particles to obtain the surface-modified bone meal. The bone meal material is superior to the conventional calcined bone meal in biocompatibility, benefits rapid adhesion and proliferation of bone cells and formation of a new bone, and shortens the repair time of a defective bone. The bone meal prepared by the preparation method provided by the invention can be applied to repair of bone tissue defects and bone nonunion and can beused as an alveolar filling material and the like.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a surface-modified bone meal and a preparation method thereof. Background technique [0002] Autologous bone grafting and allogeneic bone grafting are the main methods for the repair of bone defects. The effect of autologous bone is better, but there are limited sources, which will cause secondary trauma to patients and surgical complications. Bone allografts can cause immune rejection. The use of artificial materials to replace the former two in clinical application is the main direction in the field of bone grafting, and it is also an important method for repairing periodontal defects, bone defects, and nonunions. [0003] At present, there are many bone repair materials used in bone tissue engineering (such as metal materials, bioceramics, bone cement, nanomaterials, etc.), but the problems faced include the difficulty of replacement and absorption in the impl...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/12A61L27/56A61L27/20
CPCA61L27/12A61L27/20A61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/56A61L2430/02A61L2430/12C08L5/08
Inventor 范代娣古娟马晓轩朱晨辉段志广杨婵媛贾利平
Owner XIAN GIANT BIOGENE TECH CO LTD
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