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Frontal polymerization preparation method for thymopentin molecular imprinted hydrogel

A technology of molecular imprinting and hydrogel, which is applied in the direction of pharmaceutical formulations, drug combinations, medical preparations of non-active ingredients, etc., to achieve the effects of stable physical and chemical properties, large drug loading, and improved drug bioavailability

Active Publication Date: 2018-12-07
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention is widely used in the treatment of immunodeficiency diseases

Method used

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  • Frontal polymerization preparation method for thymopentin molecular imprinted hydrogel
  • Frontal polymerization preparation method for thymopentin molecular imprinted hydrogel
  • Frontal polymerization preparation method for thymopentin molecular imprinted hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In order to investigate the surface morphology of thymopentin molecularly imprinted hydrogels more clearly, we used wave polymerization to prepare molecularly imprinted hydrogels (FP-MIP) and non-imprinted hydrogels (FP-NIP), and thermal polymerization to prepare molecules Imprinted hydrogel (BP-MIP) and non-imprinted hydrogel (BP-NIP). The hydrogel material was analyzed by field emission scanning electron microscopy to characterize the structural characteristics of the hydrogel. The specific operation steps are as follows:

[0036] a. Preparation of Thymopentin Molecularly Imprinted Hydrogel Drug Carrier Materials by Wave Polymerization:

[0037] 1) Preparation of DES system: Mix choline chloride (4g) and ethylene glycol (10.8mL) according to the measured amount, stir magnetically in an oil bath at 80°C until a clear and uniform solution is obtained, and store at room temperature for later use.

[0038] 2) Mix thymopentin (326mg), N-isopropylacrylamide (670mg), acryl...

Embodiment 2

[0051] The thymopentin equilibrium adsorption experiment was used to study the specific adsorption performance of the molecularly imprinted hydrogel on the template molecule thymopentin. In order to investigate the specific recognition ability of the imprinted hydrogel on thymopentin, the thymopentin molecular Adsorption isotherms of hydrogel and non-imprinted hydrogel in the range of 0.5-5.0mmol / L. The specific operation steps are as follows:

[0052] a. Same as above method (Example 1) Synthetic Wave Polymerized Molecularly Imprinted Hydrogel (FP-MIP), Wave Polymerized Non-imprinted Hydrogel (FP-NIP), Thermal Polymerized Molecularly Imprinted Hydrogel (BP-MIP), Thermally polymerized non-imprinted hydrogels (BP-NIP).

[0053] b. Weigh 10.0 mg of dried hydrogel material and put them into 5ml centrifuge tubes. Then prepare an aqueous solution of 0.5-5.0mmol / L TP-5, add 3.0mL of TP-5 solutions of different concentrations to the above centrifuge tubes, soak in a water bath at 3...

Embodiment 3

[0060] MTT assay to study the cytotoxicity of hydrogel materials. In order to investigate the cytocompatibility of the hydrogel material, the relative cell proliferation rate after the cells were cultured on the hydrogel material extract for different periods of time was determined. The specific operation steps are as follows:

[0061] a. Same as above method (Example 1) Synthetic Wave Polymerized Molecularly Imprinted Hydrogel (FP-MIP), Wave Polymerized Non-imprinted Hydrogel (FP-NIP), Thermal Polymerized Molecularly Imprinted Hydrogel (BP-MIP), Thermally polymerized non-imprinted hydrogels (BP-NIP).

[0062] b. Take mouse fibroblasts grown in logarithmic phase, trypsinize, and dilute to 1×10 with DMEM medium containing 10% fetal bovine serum (FBS). 4 Concentration, add to 96-well plate, add 100 μL of cell suspension to each well, and then place the cells in a cell culture incubator (37°C, 5% CO 2 ) for 24 hours, the original culture medium was discarded, and the hydrogel ...

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Abstract

The invention relates to a frontal polymerization preparation method for a thymopentin molecular imprinted hydrogel which is prepared from the raw materials in percentage by mass: 4.33%-11.94% of thymopentin, 10.00%-36.44% of N-isopropylacrylamide, 5.88%-21.86% of acrylic acid, 0.36%-0.74% of N, N'-methylenebisacrylamide, 9.92%-33.58% of choline chloride and 30.12%-53.68% of ethylene glycol. By using the frontal polymerization preparation method, a molecular imprinted hydrogel sustained-release material is prepared by taking bioactive molecule thymopentin as a template and a green solvent DESsystem as a pore-foaming agent, and the molecular imprinted hydrogel sustained-release material serving as a drug carrier is stable in physical and chemical properties and obvious in imprinting effect; the molecular imprinted hydrogel sustained-release material is large in drug loading capacity and has an obvious sustained-release effect for the template molecule thymopentin; and compared with a thermal polymerization imprinted hydrogel, the molecular imprinted hydrogel sustained-release material is capable of prolonging the in-vivo metabolism time of a drug and increasing the biological availability of the drug.

Description

technical field [0001] The invention relates to a method for preparing thymopentin molecularly imprinted hydrogel by wave polymerization. Background technique [0002] Thymopentin (Arg-Lys-Asp-Va1-Tyr, TP-5) is a synthetic pentapeptide corresponding to the active site of thymopoietin II. Since its discovery, TP-5 has been clinically used to treat immunodeficiency diseases such as rheumatoid arthritis, cancer, hepatitis B virus infection and acquired immunodeficiency syndrome (AIDS). However, due to the extensive enzymatic degradation and poor membrane permeability, TP-5 has a very short half-life of 30 s in human plasma, so its clinical application is greatly limited. At present, TP-5 is mostly used clinically for intramuscular or intravenous injection, but studies on TP-5 oral administration are rare, because polypeptide drugs have poor stability and low absorption rate in the gastrointestinal tract, so a preparation of a Drug-loaded devices that can protect thymopentin f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08J3/075C08F2/44C08F220/06C08F220/54C08F222/38A61K9/06A61K47/18A61P29/00A61P35/00A61P31/20A61P1/16A61P31/18
CPCA61K9/0002A61K9/06A61K47/18A61P1/16A61P29/00A61P31/18A61P31/20A61P35/00C08F2/44C08F220/54C08J3/075C08J9/26C08J2333/24C08F220/06C08F222/385
Inventor 刘照胜王晓琳黄艳萍
Owner TIANJIN MEDICAL UNIV
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