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Solid culture method of cordyceps cicadae

A cultivation method, solid cultivation technology, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve problems such as energy waste, achieve the effects of reducing energy consumption, shortening the light time, and reducing the amount of sporulation

Active Publication Date: 2018-11-30
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the light time used in the artificial cultivation of cicadae is mostly 24 hours in the light cultivation stage, which causes unnecessary waste of energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The expanded cultivation of embodiment 1 cicadae

[0016] Slope culture: inoculate the strains into the inclined test tubes, then put the inclined test tubes inoculated with the strains into the incubator at 22°C, and cultivate for 7 days until the mycelium is full of the test tubes;

[0017] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;

[0018] Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, and keep the pressure for 30-45 minutes;...

Embodiment 2

[0022] The solid culture of embodiment 2 cicadae

[0023] Preparation of solid medium: After washing the wheat and controlling the water, add an appropriate amount of water, the weight ratio of which is wheat: water is 1:1.4, and mix well; after mixing well, put it into a culture container, and control the thickness of the medium at 4cm. Place the culture container filled with the culture medium in a sterilizer, and sterilize at 121°C for 30-50 minutes; after sterilization, move the culture container to the buffer room, cool naturally to below 24°C, and move it to the inoculation room.

[0024] Inoculation: prior to inoculation, the culture container was irradiated with ultraviolet light for 0.5h in a ultra-clean workbench or a class 100 laminar flow hood (down); The inoculated containers are placed in a culture room for cultivation.

[0025] Culture conditions: In the dark culture stage, the culture temperature is 22-24°C, and the relative humidity of the air is 70-85%. When...

Embodiment 3

[0026] The solid culture of embodiment 3 cicadae

[0027] The cultivation process is basically the same as that in Example 2, except that the illumination time is 16 hours.

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Abstract

The invention provides a solid culture method of cordyceps cicadae. The culture method is characterized in that according to the method, cordyceps cicada strains subjected to expanded culture are inoculated onto a solid culture medium, dark culture and light culture are carried out in sequence, and fruiting bodies are harvested timely, so that the cordyceps cicadae is obtained, wherein the illumination of 16-20 hours per day is adopted in the light culture stage.

Description

technical field [0001] The invention relates to a solid culture method for cicadas, in particular to a method for artificially cultivating cicadas by fixing the light time in the light cultivation process. Background technique [0002] The traditional Chinese medicine cicada is a complex of bacteria and insects formed by Isaria cicadae Miquel (Paecilomyces cicadae (Miquel) Samson) parasitizing on cicada nymphs. It is one of the famous traditional Chinese medicinal materials in my country. 800 years earlier. Cicada is a medicinal and edible fungus with high nutritional value and specific efficacy. Cicada can produce a variety of physiologically active substances that play an important role in medical treatment and health care, including nucleosides, cyclic peptides, polysaccharides, alcohols, sterols and organic acids, etc., in regulating immunity, improving kidney function, It has obvious effects in regulating lipid metabolism, anti-tumor, anti-fatigue, analgesia, hypnosis,...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G7/06
CPCA01G7/06
Inventor 李春如路文遥钱泽勇纪伟陈桃宝徐煜王玉芹孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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