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Polydopamine coated gold core/hollow silica shell nanometer material as well as preparation and application thereof

A nanomaterial, polydopamine technology, applied in nanotechnology, metal processing equipment, preparations for in vivo experiments, etc., can solve the problems of uneven loading of nano-venus and difficulty in controlling the shape

Active Publication Date: 2018-11-20
DONGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a polydopamine-wrapped gold core / hollow silicon shell nanomaterial and its preparation and application, to overcome the defects that the loading of the existing material nano-venus is not uniform enough and its shape is difficult to control, the method The prepared nano-platform has excellent biocompatibility and stability, and has good US / CT / PA imaging and photothermal therapy effects, which provides a new way for the development of multimodal imaging contrast agents and integrated platforms for diagnosis and treatment. method with broad application prospects

Method used

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  • Polydopamine coated gold core/hollow silica shell nanometer material as well as preparation and application thereof
  • Polydopamine coated gold core/hollow silica shell nanometer material as well as preparation and application thereof
  • Polydopamine coated gold core/hollow silica shell nanometer material as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0089] The specific process of preparing the ASPP nano-platform is as follows: figure 1 shown.

[0090] (1) Take 30ml of 1mM chloroauric acid solution and heat it to 100°C while stirring, then quickly inject 3ml of sodium citrate solution with a mass fraction of 1%, continue heating and reflux for 20-30min, and cool the solution to room temperature , to obtain a gold colloidal solution. into the gold colloidal solution to facilitate subsequent coating of the silica layer, and stirred and reacted at room temperature for 24 hours. The product was collected by centrifugation.

[0091] (2) Add 0.235ml polyvinylpyrrolidone solution (12.8g / L) to the gold colloid solution in (1), and stir at room temperature for 24h to obtain PVP-modified gold nanoparticles, which are collected by centrifugation.

[0092] (3) Disperse the product obtained in (2) into a mixture of ammonia water (0.62ml), absolute ethanol (13.6ml) and deionized water (3.3ml), ultrasonically disperse for 10min, then...

Embodiment 2

[0098] The product obtained in (7) in Example 1 was configured with sterile PBS buffer to form a mother solution with a gold concentration of 48 μg / ml, and then serially diluted to 32, 16, 8, 4, and 2 μg / ml. The cultured 4T1 cells were planted in a 96-well plate, and inoculated at a density of 10,000 cells / well, with a volume of 100 μL per well. After culturing overnight, wash 2-3 times with PBS, then add the above-mentioned materials of each dilution gradient, and co-culture with the cells for 24 hours. Five parallel wells were made for each gradient, and PBS buffer was used as a blank control. After the culture, wash with 100 μL PBS three times, then add 90 μL serum-free medium and 10 μL CCK8 solution to each well, incubate at 37 °C for 2 h, and detect the absorbance at 450 nm with a microplate reader. The results of cell viability detected by CCK-8 method showed that ASPP did not show obvious cytotoxicity and showed good cytocompatibility ( Figure 6 ).

Embodiment 3

[0100] The product obtained in (7) in Example 1 was prepared with ultrapure water into materials with a gold concentration of 20mM and 40mM, filled with 2mL PE tubes respectively, and B-mode ultrasonic imaging tests were performed on the two groups of materials. Ultrapure water was used as blank control. The test results show that: within the test concentration range, ASPP exhibits excellent US imaging effects ( Figure 7 ).

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Abstract

The invention relates to a polydopamine coated gold core / hollow silica shell nanometer material as well as preparation and application thereof. The nanometer material is prepared according to the following steps: coating gold nanoparticles and perfluorohexane (PFH) in the inner cavity of hollow mesoporous silica, and coating polydopamine on the surface of hollow mesoporous silica; stably reducingHAuCl4 with sodium citrate so as to obtain gold nanoparticles, taking ethyl orthosilicate as a silicon source, forming mesoporous solid silicon-coated gold nanoparticles in a mixed solution of ethanol, ultrapure water and ammonium hydroxide, and etching gold core / hollow silica shell nanoparticles with sodium carbonate; modifying amino on the surface of the nanoparticles, and coating perfluorohexane; finally, coating a polydopamine layer on the surface of the nanoparticles, thereby obtaining the product. The nano platform prepared by the method disclosed by the invention has good stability andexcellent biocompatibility, also has good US / CT / PA imaging and photothermal therapy effects, provides a novel method for development of the multimode imaging contrast agent and diagnosis and treatmentintegrated platform, and is wide in application prospects.

Description

technical field [0001] The invention belongs to the field of hollow mesoporous nanocomposite materials and their preparation and application, in particular to a polydopamine-wrapped gold core / hollow silicon shell nanomaterial and its preparation and application. Background technique [0002] Common molecular imaging methods include Ultrasound Imaging (US), X-ray computed tomography imaging (CT) and Photoacoustic Imaging (PA). Ultrasound imaging has continuous dynamic imaging, but its resolution and sensitivity are low; CT imaging has the advantages of short image acquisition time, high spatial resolution, low price, and flexible reconstruction of 3D images, but there are many reconstructed image artifacts; Acoustic imaging has good anti-interference, but deep tissue imaging is greatly affected by absorption. These three imaging modes have their own advantages and disadvantages. If multiple different types of imaging elements are incorporated into a single nanoparticle syste...

Claims

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Application Information

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IPC IPC(8): A61K49/22A61K41/00A61P35/00B22F9/24B22F1/02B82Y40/00
CPCA61K41/0052A61K41/0057A61K49/221A61K49/225A61P35/00B82Y40/00B22F9/24B22F1/16
Inventor 沈明武蔡超李鑫张昌昌刘梦雪欧阳智俊史向阳彭琛夏进东王玥
Owner DONGHUA UNIV
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