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Application of photochemically-induced cerebral arterial thrombosis model for zebra fish

An ischemic stroke and zebrafish technology, applied in the field of medical animal model research, can solve the problems of severe thrombus reflection, difficult to observe the antithrombotic effect of drugs, unsuccessful thrombus realization, etc., achieving good repeatability and simple and appropriate construction method. , the effect of short cycle

Pending Publication Date: 2018-11-06
NANJING UNIV OF TECH
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AI Technical Summary

Problems solved by technology

Ferric chloride triggers free radical bursts, induces severe thrombosis reaction, and it is difficult to observe the antithrombotic effect of drugs
Phenylhydrazine induction of thrombus has not been successfully achieved

Method used

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  • Application of photochemically-induced cerebral arterial thrombosis model for zebra fish
  • Application of photochemically-induced cerebral arterial thrombosis model for zebra fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1. Anesthetize the zebrafish

[0028] Adult zebrafish of a wild-type strain with normal development were selected and anesthetized with 0.16 mg / ml tricaine aminobenzoate ethyl methylsulfonate (MS-222).

[0029] 2. Photochemical Induction Treatment

[0030] According to the body weight of each zebrafish, the dose of rose bengal solution was injected into its abdomen at a dose of 100 μg / g, and the total injection volume did not exceed 20 μl. The control group was injected with an equal volume of 0.9% normal saline. After 10 minutes, the zebrafish was transferred to a solution containing 75 mg / l tricaine anthranilate ethyl ester methanesulfonate, and the entire optic tectum area of ​​the head was exposed to the light source for 20 minutes, except the head area, The rest of the parts were covered with tinfoil, and after irradiation, they were transferred to normal containers for recovery, and the containers had to be placed in a dark room for observation.

[0031] 24 hour...

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Abstract

The invention discloses an application of a photochemically-induced cerebral arterial thrombosis model for zebra fish. The cerebrum tissue is taken as an identification index of cerebral arterial thrombosis model for zebra fish. The principle of forming photochemically-induced thrombus is adopted. A rose-bengal solution is injected to abdominal cavities of zebra fish. Therefore, complete local infarction in cortex areas of zebra fish is caused by illumination within short time. Accordingly, an in vivo animal model is established. In the model, blood coagulation is caused by damaging vascular endothelial cells due to singlet oxygen released by the rose-bengal solution under light irradiation. The pathological process of cerebral arterial thrombosis is effectively simulated. According to anexperiment result of an observational index, the cerebrum area tissue of zebra fish has evident loose and infarct phenomena. Swimming behaviors of dysneuria such as whirling and standing upright occurto zebra fish. The method is simple and high in success rate and convenient to promote. Damage sensitivity of cerebrum tissue is high and repeatability is good. The photochemically-induced cerebral arterial thrombosis model for zebra fish can act as a practical tool index for screening medicine, for blood vessel protection after cerebral infarction and thrombolytic therapy.

Description

technical field [0001] The invention relates to the application of a photochemically induced zebrafish ischemic stroke model, belonging to the field of medical animal model research. Background technique [0002] Stroke, commonly known as "stroke", is an acute disease caused by disease in the blood vessels that supply blood to the brain. Ischemic stroke is the disorder of blood supply in local brain tissue caused by various reasons, resulting in ischemic and hypoxic lesions and necrosis of brain tissue, which in turn leads to clinically corresponding neurological deficits. [1] . [0003] Stroke is a common and refractory cardiovascular and cerebrovascular disease that seriously endangers human health and life safety. Together with tumor diseases and respiratory diseases, it is one of the three diseases with the highest human mortality rate, and it is also the most developed among the three diseases. The disease with the fastest recovery, the slowest recovery, the most deat...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 何明芳张晓欢李崇勇
Owner NANJING UNIV OF TECH
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