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Bacillus py-3 and its agent and application

A PY-3, Bacillus technology, applied in bacteria, chemical instruments and methods, biochemical equipment and methods, etc., to achieve the effect of improving sewage water quality, reducing corrosion, and meeting design requirements

Active Publication Date: 2021-07-13
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The fourth object of the present invention is to apply the microbial strain, its bacterial agent or fermented liquid in the oilfield polymer flooding to treat the sewage of the prepared polymer, solve the problem of polymer solution viscosity reduction caused by sulfide, and improve the polymerization rate. oil displacement effect

Method used

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  • Bacillus py-3 and its agent and application
  • Bacillus py-3 and its agent and application
  • Bacillus py-3 and its agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Screening of Bacillus sp. PY-3 (CGMCC No.15606) strain.

[0035] Take the sewage from the Gudao oil production plant in Shengli Oilfield, take 1ml with a disposable syringe, and insert it into a solid medium (the composition of the medium: Na 2 S·9H 2 O 0.005wt%, anhydrous sodium acetate 0.3wt%, NaHCO 3 0.1wt%, NH 4 Cl 0.1wt%, KNO 3 0.2wt%, K 2 HPO 4 0.1wt%, agar 2%, the balance is water, pH value is 7.0, 80% nitrogen is blown off to ensure anaerobic environment in the whole process of preparing the medium), 37 ° C constant temperature culture for 7 days until a single colony appears on the medium . Pick a single colony on the medium and transfer it to an anaerobic liquid medium with the same composition for expanded culture, and culture at a constant temperature of 37°C for 7 days. Take the culture solution and insert it into the anaerobic solid medium again, culture at a constant temperature of 37°C for 7 days, and isolate a single colony.

[0036] Each singl...

Embodiment 2

[0039] Morphological, physiological and biochemical characteristics of Bacillus sp. PY-3 (CGMCC No.15606).

[0040] 1. Test strains

[0041] Bacillus sp. PY-3 (CGMCC No. 15606) strain isolated in Example 1 of the present invention.

[0042] 2. Experimental method

[0043] Refer to the experimental method of "Bergey's Manual of Systematic Bacteriology" to detect its Gram staining, cell size and shape, growth temperature, growth pH range, and NaCl tolerance. Perform methyl red, catalase, V-P, glucose acid production, starch hydrolysis, pyocyanin, oxidase experiments, nitrate reduction, nitrite reduction experiments, and use of acetate, citrate, arabinose, galactose , xylose, inosose, lactose, rhamnose and sucrose experiments.

[0044] 3. Experimental results

[0045] The results showed that the bacterium was Gram-positive, rod-shaped under the microscope, facultative anaerobic, the growth temperature was 30-50°C, the most suitable growth temperature was 37°C, the growth pH r...

Embodiment 3

[0047] Bacillus sp. PY-3 (CGMCC No.15606) performance experiment on sulfide removal.

[0048] 1. Test strains

[0049] Bacillus sp. PY-3 (CGMCC No. 15606) strain isolated in Example 1 of the present invention.

[0050] 2. Experimental method

[0051] Pick a single colony of the tested strain and insert it into 100 ml of anaerobic liquid medium for overnight cultivation at 37° C. to the logarithmic growth phase. The composition of the medium is the same as in Example 1. Prepare oilfield sewage with a sulfide content of 30mg / L, inoculate the cultivated bacterial solution into a triangular flask containing 100ml of oilfield sewage at an inoculation amount of 2%, place it in a 37°C incubator, and test the sulfide in it every 12 hours. The concentration of sulfide, the original water sample without inoculation treatment was used as the control in the experiment.

[0052] 3. Test results

[0053] Test results such as image 3 shown. Under the condition of 37 ℃, the sulfide con...

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Abstract

The invention discloses a strain of Bacillus sp. PY-3 and its bacterial agent and application. The strain of the present invention is obtained from the sewage sample of Gudao oil production plant in Shengli Oilfield, and is obtained by enrichment culture screening at 37° C. under anaerobic conditions , and was obtained by repeated screening and passage through the desulfurization performance investigation, which was named Bacillus sp.PY‑3, and its deposit number was CGMCC No.15606. The invention discloses its application in oilfield sewage treatment. The bacillus or its bacterial agent of the present invention can effectively remove sulfide in sewage, reduce corrosion and improve sewage water quality. For the treatment of polymer sewage prepared in polymer flooding in oilfields, the bacillus or its bacterial agent of the present invention can effectively solve the problem of viscosity reduction caused by sulfide, and ensure the development effect of polymer flooding.

Description

technical field [0001] The invention belongs to the fields of oilfield sewage treatment technology and environmental biotechnology, and specifically relates to a strain of bacillus PY-3 and its application in oilfield sewage treatment. Background technique [0002] With the continuous development of thermal recovery and water injection of heavy oil, a large number of produced fluids with high sulfide content have appeared in various oil fields. The existence of sulfide in oilfield wastewater will cause various hazards. Sulfide in sewage will cause severe corrosion of equipment, pipelines and other metal materials, resulting in equipment damage, and the black precipitation of ferrous sulfide, the corrosion product, will cause suspended solids in sewage to rise and form dirt, resulting in deterioration of water quality. At the same time, during the application of polymer flooding technology, due to the sulfide contained in the reinjected sewage, the viscosity of the prepared ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F103/36C02F101/10
CPCC02F3/345C02F2103/365C12N1/20C12N1/205C12R2001/07
Inventor 束青林徐鹏徐闯张守献潘永强袁长忠冯逸茹曹嫣镔宋永亭林军章
Owner CHINA PETROLEUM & CHEM CORP
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