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Solid culture medium for marasmius androsaceus and preparation method of dried marasmius androsaceus thalli

A technology of solid medium and Anluo small leather umbrella, which is applied in botany equipment and methods, cultivation, plant cultivation, etc. It can solve the problems of low content of effective substances, influence on cell reproduction, high manufacturing cost, etc., and achieve the goal of increasing the production of effective substances The content and growth speed have obvious advantages, and the effect of reducing production costs

Inactive Publication Date: 2018-10-09
湖北欣恺生物科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

In the existing culture medium, due to the addition of rice husks to increase the air permeability of the culture medium, it is convenient for the growth of Pymella pilosula mycelium, so that the protein content (nitrogen source) with wheat bran as the main raw material and the carbon with white granulated sugar as the main material source, the nutrients that can be provided are limited, affecting the reproduction of cells
Too low a carbon-to-nitrogen ratio is not conducive to cell growth and the expression and accumulation of foreign proteins, leading to early autolysis of the bacteria, and too high a balance of cell metabolism, which is ultimately not conducive to the accumulation of metabolites
In addition, although the inorganic salts that are beneficial to the growth of Anluo Xiaopi umbrella are provided in the existing culture medium and include potassium, magnesium, phosphorus, etc., they still lack certain elements or insufficient content, which will continue to affect the growth and reproduction of microorganisms. The synthesis and catalytic activity of biological enzymes make the metabolic reactions unable to proceed normally
However, when the existing culture medium is in use, there are following defects: 1. The dried thallus of P. angustifolia obtained by using the solid culture medium has a general growth rate; 2. The content of ethanol extract and water-soluble matter in the metabolites The proteoglycan content and other effective substances in the medium are low; 3. Various identification reactions are not obvious, and the drug effect is not good; 4. Therefore, the manufacturing cost is high, and the drug effect is poor, etc.
On this basis, its concentration was optimized, and the composition of the culture medium of Dermella parsley was optimized through orthogonal experiments, and the optimal medium was finally established: 3% corn flour, 3% bran, MgSO 4 0.1%, KH 2 PO 4 0.3%, VB110mg, water 1000ml, this medium has been screened for C source and N source species, but only for liquid fermentation medium

Method used

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  • Solid culture medium for marasmius androsaceus and preparation method of dried marasmius androsaceus thalli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for preparing the dried thalline of Pseudaria pilosula and the inspection of the finished product by using solid medium is carried out according to the following steps:

[0027] 1. Preparation and sterilization of solid medium: Weigh 47kg of wheat bran, 25kg of corn flour, 2.5kg of soybean meal, 0.5kg of calcium sulfate powder, 25kg of rice husk, 1kg of white sugar, 0.1kg of potassium dihydrogen phosphate, and 0.1kg of magnesium sulfate , add 80kg of water, mix thoroughly, pack into bags or bottles, and sterilize at 121°C for 2 hours;

[0028] 2. Inoculation: In the C+A clean area, inoculate according to 20% of the weight of the solid medium, and inoculate the liquid seeds of Anluo Xiaopi umbrella on the solid medium prepared in step 1;

[0029] 3. Cultivation: Cultivate the solid medium that has been inoculated in step 2 for 50 days at a temperature of 28°C;

[0030] 4. Drying: cut the solid medium cultivated in step 3 into pieces, and dry at 100°C for 18 h...

Embodiment 2

[0035] The method for preparing the dried thalline of Pseudaria pilosula and the inspection of the finished product by using solid medium is carried out according to the following steps:

[0036] 1. Preparation and sterilization of solid medium: Weigh 47kg of wheat bran, 23kg of corn flour, 4kg of soybean meal, 1kg of calcium sulfate powder, 25kg of rice husk, 1kg of white sugar, 0.1kg of potassium dihydrogen phosphate, 0.1kg of magnesium sulfate, add water 80kg, mix well, pack into bags or bottles, and sterilize at 121°C for 2 hours;

[0037] 2. Inoculation: In the C+A clean area, inoculate according to 19% of the weight of the solid medium, and inoculate the liquid seeds of Anluo Xiaopi umbrella on the solid medium prepared in step 1;

[0038] 3. Cultivation: Cultivate the solid medium that has been inoculated in step 2 for 50 days at a temperature of 27°C;

[0039] 4. Drying: cut the solid medium cultivated in step 3 into pieces, and dry at 100°C for 19 hours to obtain dri...

Embodiment 3

[0042] The method for preparing the dried thalline of Pseudaria pilosula and the inspection of the finished product by using solid medium is carried out according to the following steps:

[0043] 1. Preparation and sterilization of solid medium: Weigh 50kg of wheat bran, 22kg of corn flour, 2.25kg of soybean meal, 0.75kg of calcium sulfate powder, 25kg of rice husk, 1kg of white sugar, 0.1kg of potassium dihydrogen phosphate, and 0.1kg of magnesium sulfate , add 80kg of water, mix thoroughly, pack into bags or bottles, and sterilize at 121°C for 2 hours;

[0044] 2. Inoculation: In the C+A clean area, inoculate according to 18% of the weight of the solid medium, and inoculate the liquid seeds of Anluo Xiaopi umbrella on the solid medium prepared in step 1;

[0045] 3. Cultivation: Cultivate the solid medium that has been inoculated in step 2 for 50 days at a temperature of 26°C;

[0046] 4. Drying: cut the solid culture medium cultivated in step 3 into pieces, and dry at 100°...

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Abstract

The invention provides a solid culture medium for marasmius androsaceus and a preparation method of dried marasmius androsaceus thalli. The solid culture medium comprises the following components in parts by weight: 40-55 parts of wheat bran, 18-25 parts of corn flour, 2-5 parts of soybean meal, 0.5-2 parts of calcium salt powder, 20-30 parts of rice husk, 1-5 parts of white granulated sugar, 0.01-0.2 part of potassium dihydrogen phosphate, 0.01-0.2 part of magnesium sulfate and 80-100 parts of water. The preparation method comprises the following steps: 1, preparing and sterilizing the solidculture medium; 2, inoculating; 3, culturing; 4, drying. In the dried marasmius androsaceus thalli cultured by utilizing the culture medium, the contents of effective substances such as proteoglycan in an ethanol extract and a water-dissolved product of the marasmius androsaceus are greatly increased; the growth speed has an obvious advantage.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a method for preparing an Anluo Xiaopi solid culture medium and dried bacteria. Background technique [0002] Anluotong dried fungus is the main ingredient in the production of Anluotong Tablets. Anluotong has the functions of dredging meridians, activating collaterals, promoting blood circulation and relieving pain. The hyphae and mycelia of Anluo Picobacteria contain proteoglycans, mannitol, amino acids, p-hydroxycinnamic acid, cholesterol, octadecanoic acid and other chemical components. The dry fungus of Pygmycus anrophyllum is obtained by culturing the fungus of the genus Pyrotium anrophyllum of the genus Pyremycota angustifolia through solid medium culture and drying. Conventional solid media are wheat bran, rice hulls, water. According to relevant information, the components of the optimized solid medium are wheat bran, white sugar, rice hulls, potassium dihydrogen phos...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20
Inventor 董平刘华杨阳代堂慧
Owner 湖北欣恺生物科技有限公司
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