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A method for the simultaneous determination of 22 flavonoids and phenolic acids in citrus fruits

A technology of phenolic acids and fruits, which is applied in the field of chemical analysis to achieve the effect of simple operation, high sensitivity and good repeatability

Active Publication Date: 2020-10-02
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

In the existing research, there is no method for the detection of flavonoids and phenolic acids by high performance liquid chromatography with diode array detector and fluorescence detector

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  • A method for the simultaneous determination of 22 flavonoids and phenolic acids in citrus fruits
  • A method for the simultaneous determination of 22 flavonoids and phenolic acids in citrus fruits
  • A method for the simultaneous determination of 22 flavonoids and phenolic acids in citrus fruits

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Embodiment Construction

[0015] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0016] A method for simultaneously measuring 22 kinds of flavonoids and phenolic acids in citrus fruits, comprising the following steps:

[0017] 1) Preparation of sample solution: Weigh 10 citrus fruits, dry the peel or pulp (dried or freeze-dried), crush them, and pass through a 60-mesh sieve; weigh 0.1~0.5g, add 20mL, 50wt.% methanol solution to ultrasonic Extract for 20 minutes and centrifuge; after repeated extraction twice, combine the supernatants, add 50wt.% methanol solution to make up to 50mL, centrifuge at 8000rpm / min, filter the supernatant through a 0.22μm organic filter membrane to obtain a sample solution, and set aside;

[0018] 2) Preparation of mixed standard samples: gallic acid, synephrine, chlorogenic a...

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Abstract

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.

Description

technical field [0001] The invention belongs to the field of chemical analysis, in particular to a HPLC-DAD-FLD method for simultaneously measuring 22 kinds of flavonoids and phenolic acids in citrus fruits. Background technique [0002] Citrus is the world's largest fruit and one of the main fruits grown in China. In 2015, my country's citrus cultivation area reached 2.513 million hectares, ranking first in the world, and its output was 36.6008 million tons, ranking third in the world. Citrus planting occupies a very important position in the rural economy of southern my country. Citrus is deeply loved by people for its high nutritional value and unique taste. Citrus is rich in phenolic substances, which not only have antibacterial, anti-inflammatory, anti-allergic, analgesic, prevent and treat cardiovascular diseases, anti-virus, anti-oxidation, anti-cancer and other medicinal and edible values, but also one of the important indicators of citrus fruit maturity. one. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈源余文权杨道富杨成龙张迪
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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