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Silk fibroin hydrogel

A technology of silk fibroin and hydrogel, which is applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve the problem of poor biocompatibility, high osmotic pressure of small molecular weight polyethylene glycol, and inability to reflect the biological phase of silk fibroin. Capacitance and other issues

Inactive Publication Date: 2018-08-17
SUZHOU SIMEITE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention patent of Chinese Patent Publication No. CN 106267370 A discloses a silk fibroin / cellulose 3D printing ink using genipin as a cross-linking agent. Although the article discloses that the material can be used to embed cells, however Tests have proved that genipin has poor biocompatibility, resulting in insufficient overall bioidentity of the material, which cannot be used for embedding cells, and its chemical cross-linking is complex, and its biological safety is yet to be confirmed
[0010] The Chinese Patent Publication No. CN 106362208 A discloses a silk fibroin / xanthan gum hydrogel, which uses silk fibroin powder instead of regenerated silk fibroin. Cross-linking does not form cross-linking between silk fibroin. At this time, silk fibroin powder is no different from other particles that can be cross-linked, and cannot reflect the good biocompatibility of silk fibroin. At the same time, silk fibroin powder The surface is hydrophobic, which is not conducive to cell adhesion and growth
[0011] Chinese Patent Publication No. CN 103289107 A invention patent discloses a silk fibroin in situ gel, which uses a single small molecular weight polyethylene glycol to induce silk fibroin gel, but the low molecular weight polyethylene glycol has a high osmotic pressure , causing the silk fibroin gel to inhibit cell adhesion and proliferation
[0012] The Chinese publication number is CN 101772348 B, which uses ultrasonic method to prepare silk fibroin gel. The silk fibroin hydrogel is not injectable, and when the silk fibroin concentration is higher than 4%, the cells cannot proliferate normally.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides an experimental process and results of the effect of different molecular weight polyethylene glycols on cell proliferation, as follows:

[0041] S1. Refrigerated P2 human bone marrow mesenchymal stem cells were inoculated into T175 cell culture flasks, and cultured in DMEM medium containing 10% FBS and 1% double antibody. When the cells expanded to cover 80% of the bottom of the culture flask, they were washed twice with PBS, digested with 2 mL of 0.25% trypsin, and added 4 mL of cell culture medium to terminate the digestion. Centrifuge, resuspend in cell culture medium, and count.

[0042] S2, 5000 cells / well were seeded in a 24-well cell culture plate, and cultured in DMEM medium containing 10% FBS and 1% double antibody for 12 hours.

[0043] S3. Discard the supernatant, add culture solutions containing different molecular weights and a mass concentration of 5% polyethylene glycol, and continue to cultivate. When culturing for 1 day, 2 days...

Embodiment 2

[0055] This example provides a silk fibroin hydrogel prepared by different molecular weight polyethylene glycols and their combinations, and uses scanning electron microscopy to study the three-dimensional structure of the hydrogel to detect the effects of different molecular weight polyethylene glycols on silk fibroin. Influence of protein gel structure. Specific steps are as follows:

[0056] S1. Cut silkworm cocoons into pieces and weigh 100 grams, boil in 40L of 0.02M sodium carbonate aqueous solution for 30 minutes to remove sericin, then repeatedly wash with deionized water for 3 times, put into fume hood after washing Dry and set aside.

[0057] S2. Weigh 10 grams of degummed silk, place it in 40 mL of 9.3 M lithium bromide solution, and treat it at 60° C. for 4 hours. Place in a dialysis device and dialyze against deionized water for 48 hours, and centrifuge to remove insoluble particles.

[0058] S3. Dilute the regenerated silk fibroin solution obtained in S2 to a ...

Embodiment 3

[0071] This example provides a silk fibroin solution that acts on a large molecular weight polyethylene glycol while adding small molecule polyethylene glycol to promote silk fibroin to form a hydrogel. The results of the gelation speed are compared, specifically as follows step:

[0072] S1. Cut silkworm cocoons into pieces and weigh 100 grams, boil in 40L of 0.02M sodium carbonate aqueous solution for 30 minutes to remove sericin, then repeatedly wash with deionized water for 3 times, put into fume hood after washing Dry and set aside.

[0073] S2. Weigh 10 grams of degummed silk, place it in 40 mL of 9.3 M lithium bromide solution, and treat it at 60° C. for 4 hours. Place in a dialysis device and dialyze against deionized water for 48 hours, and centrifuge to remove insoluble particles.

[0074] S3. Adding high molecular weight polyethylene glycol to the silk fibroin solution obtained in step S2, wherein the mass ratio of silk fibroin to polyethylene glycol is 1:1.

[0...

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Abstract

The invention relates to a hydrogel material, and in particular relates to silk fibroin hydrogel; the silk fibroin hydrogel provided by the invention is the silk fibroin hydrogel prepared from a silkfibroin solution under the action of an inducer containing large molecular weight polyethylene glycol, wherein the large molecular weight polyethylene glycol refers to polyethylene glycol having a weight average molecular weight of 3,000 to 8,000; the silk fibroin hydrogel provided by the invention has good biocompatibility, can promote cell proliferation, is matched with natural tissue in mechanical strength, injectable, and easy to implant, and has no toxic and side effects.

Description

technical field [0001] The invention relates to a hydrogel material, in particular to a silk fibroin hydrogel Background technique [0002] For the treatment of defective or unsightly tissues (such as from trauma, surgical resection or congenital defects), there are two types of tissue sources: autologous tissue transplantation and artificial filling; from the implantation method, it can be divided into window implantation and implantation. Injection fills both. Autologous tissue is limited by sources and is difficult to be widely used, and it will cause secondary trauma to the injured. Artificial filling materials are more and more widely used in clinical practice; injection filling, due to its small wound, simple operation, and less pain for patients, etc. It is widely used in the fields of filling and cosmetic plastic surgery after soft tissue trauma or surgical resection. Therefore, the research and development of injectable soft tissue filling materials has become a r...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/26A61L27/52
CPCA61L27/18A61L27/227A61L27/26A61L27/52A61L2400/06A61L2430/34C08L89/00C08L71/02
Inventor 王晓沁郑兆柱
Owner SUZHOU SIMEITE BIOTECH CO LTD
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