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Breeding medium of potato stem segment and application method thereof

A medium and potato technology, applied in the directions of application, horticultural methods, botanical equipment and methods, etc., can solve the problems of affecting the reproduction and regeneration rate of stem segments, unsatisfactory and other problems, and achieve easy industrial operation, small growth obstruction, and easy preparation. Effect

Active Publication Date: 2018-08-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although, the medium component that such method obtains is simple, and preparation is easy, but its culture effect, especially for the effect of taking root and strong seedling in the stem section propagation process, is not ideal, thereby finally affects the regeneration rate of stem section propagation

Method used

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  • Breeding medium of potato stem segment and application method thereof
  • Breeding medium of potato stem segment and application method thereof
  • Breeding medium of potato stem segment and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The preparation of potato stem section propagation medium and the effect comparison of rooting and strong seedlings of different composition medium

[0031] Potato stem segment propagation medium, the medium formulation is: MS medium, 0.05mg / L indole acetic acid (IAA), 3g / L G3251, 3g / L polyvinylpyrrolidone (PVP) and 1% sucrose.

[0032] The preparation method of the medium is: weigh 4.43g of MS medium powder (according to the amount provided in the manual), 3g of PVP powder, 10g of sucrose, add appropriate amount of distilled water to dissolve, pipette gun to draw 50ul of 1mg / L IAA into the solution , then add distilled water to make up to 1 liter (L), adjust the pH to 5.8 with 1mg / ml sodium hydroxide (NaOH), weigh 3g of G3251 powder, and heat it in a microwave oven until G3251 is fully dissolved (generally 1L needs to be heated for 7 -8 minutes). The prepared culture is subpackaged and set aside.

[0033] In order to compare the effects of different medi...

Embodiment 2

[0045] Embodiment 2: the method for the stem segment propagation of potato

[0046] Rooting culture: Take the sterile seedlings that have been cultivated for 30 days, use tweezers to pick up the stem segments and place them on the inoculation plate, cut the stem segments 2-5cm with a scalpel, and at least 2 nodes, cut a total of 36 stem segments, and place them in a sterile After the rooting medium, a node cutting was inserted into the medium to induce rooting.

[0047] The formula of rooting medium is: MS, indole acetic acid (IAA) 0.05mg / L, 3g / L G3251, 3g / LPVP, 1% sucrose, PH=5.8. Culture conditions: temperature 24°C, light intensity: 3000-5000lx, light time 16h / d, sterile culture in light incubator. Cultured for 4 weeks.

[0048] Seedling hardening: When the test tube seedlings in the rooting medium are 7-10cm high, the tissue cultured seedlings are hardened indoors. The hardening temperature is 26°C, the light intensity is 5000-10000lx, the light time is 15-17h / d, and the...

Embodiment 3

[0050] Embodiment 3: obtain the method for stem segment propagation again with embodiment 2 rooting culture to obtain tissue culture seedling

[0051] Get the aseptic seedlings of Example 2 rooting culture and amplify the propagation, clamp the stem section with tweezers and place it on the inoculation dish, cut the stem section 2-5cm with a scalpel, at least 2 nodes, and cut 52 stem sections in total and place them on the In the sterilized rooting medium, a node cutting is inserted into the medium to induce rooting.

[0052] The formula of rooting medium is: MS, indole acetic acid (IAA) 0.05mg / L, 3g / L G3251, 3g / LPVP, 1% sucrose, PH=5.8. Culture conditions: temperature 24°C, light intensity: 3000-5000lx, light time 16h / d, sterile culture in light incubator. Cultured for 5 weeks.

[0053] 2. Seedling hardening: When the test-tube seedlings in the rooting medium are 7-10cm high, harden the tissue cultured seedlings indoors. Expose hardening for 5 days.

[0054] 3. Transplant...

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Abstract

The invention relates to a breeding medium of a potato stem segment and an application method thereof. The propagation medium of the potato stem segment comprises a MS medium, indole acetic acid, vegetable gel, polyvinylpyrrolidone and sucrose. The medium has simple composition, is easy to prepare, has high homogenization degree, and is suitable for industrialization operation. By a suitable ratioof the MS, the indole acetic acid, G3251, the PVP (polyvinylpyrrolidone) and the sucrose, the potato stem segment can obtain a good rooting and seedling strengthening effect on the medium, and is suitable for a subsequent one-step culture breeding method. At the same time, the present invention also provides a preparation method and a specific application method of the propagation medium of the potato stem segment.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a propagation medium and a tissue culture method for potato stem segments. Background technique [0002] The potato (Solanum tuberosum L.) belongs to the Solanaceae Solanales; Solanaceae Solanaceae; Solanum Solanum. Potato (S.tuberosum) is also known as potato, yam egg, artichoke, etc., and its tubers are edible. It is a very important multi-purpose economic crop suitable for food, vegetables, feed, and industrial raw materials in the world. It is the fourth largest crop in the world after wheat, rice, and corn. By 2020, the demand for potatoes will increase to 40%, which will exceed the growth rate of rice, wheat, and corn. Especially in Asia and Africa, the demand for potatoes will grow faster. Potatoes are widely distributed, have strong applicability, high yield and rich nutrition, and have multiple uses such as fresh food and processing. In order...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 覃瑞张丹丹李刚刘虹张丽
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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