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Preparation method of oral inactivated porcine epidemic diarrhea virus microcapsule

A technology for porcine epidemic diarrhea and microcapsules, which is applied in microcapsules, biochemical equipment and methods, viruses, etc., to achieve the effect of preventing virus infection by oral immunization

Pending Publication Date: 2018-06-05
姜新鹏
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still little research on the prevention and control and application of animal diseases, especially in the prevention and control of pig diseases, only the PEDV neutralizing antigen COE protein expressed by E.
Does not use microencapsulated preparations to encapsulate inactivated microorganisms, including porcine inactivated pathogenic bacteria and viruses

Method used

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  • Preparation method of oral inactivated porcine epidemic diarrhea virus microcapsule
  • Preparation method of oral inactivated porcine epidemic diarrhea virus microcapsule
  • Preparation method of oral inactivated porcine epidemic diarrhea virus microcapsule

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specific Embodiment approach 1

[0025] Specific embodiment one: the microcapsule preparation method of oral inactivation porcine epidemic diarrhea virus of the present embodiment, it is carried out according to the following steps:

[0026] 1. Subculture the infected porcine epidemic diarrhea virus strain. After subculture, 40-50% of the cells have cytopathic effects. Collect the cultured virus strains and place them in a -80°C ultra-low temperature refrigerator and repeatedly freeze and thaw at room temperature 3 times to fully lyse the cells, then centrifuge at 2000-4000r / min for 10min, and absorb the supernatant, which is the porcine epidemic diarrhea virus-infected liquid;

[0027] 2. Take the porcine epidemic diarrhea virus liquid infected in step 1 and add it to the sodium alginate liquid, mix well; drop it into the mixed solution of calcium chloride and chitosan acetic acid at a constant speed; Stir at a constant speed for 1 min to make the film-forming reaction fully, and let it stand at room tempera...

specific Embodiment approach 2

[0028] Embodiment 2: This embodiment is different from Embodiment 1 in that: the infected porcine epidemic diarrhea virus strain is the JL2017 strain. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0029] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the subculture of the infected porcine epidemic diarrhea virus strain refers to the continuous subculture of the JL2017 strain on Vero-CCL81 cells. Others are the same as in the first embodiment.

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Abstract

The invention provides a preparation method of an oral inactivated porcine epidemic diarrhea virus microcapsule and relates to the field of preparation of microcapsules. The invention aims at overcoming the technical defects in an oral inactivated vaccine in domestic porcine epidemic diarrhea virus prevention and control at the present stage and provides a preparation method for the oral inactivated porcine epidemic diarrhea virus microcapsule based on inactive porcine epidemic diarrhea virus and oral microcapsule preparation technologies. Animal experimental results find that a serum neutralizing antibody titer detection result shows that the microcapsule vaccine can induce piglet mucosal immunization and systemic immune response, and serum and mucosa antibodies with relatively high levelare produced, so that the microcapsule vaccine is objectively explained to have a certain slow-release effect. After a microcapsule vaccine antigen substance is orally taken by piglets, expression ofspecific antibodies IgG and IgA of the piglets can be induced, resistance of the piglets on PEDV is improved, death rate of the piglets is reduced, and toxicity attack protection rate is 60-75% higher than that of a control group.

Description

technical field [0001] The present invention relates to methods such as inactivation technology and microcapsule preparation technology of epidemic diarrhea virus, in particular to a preparation method of microcapsules for oral inactivation of porcine epidemic diarrhea virus. Background technique [0002] Infection with porcine epidemic diarrhea virus (PEDV) is one of the main causes of porcine viral diarrhea (PED), which is characterized by severe enteritis, vomiting, watery diarrhea, and weight loss. The disease occurs throughout the year, especially in winter, and pigs of all ages are susceptible to infection, but it is the most harmful to piglets and has the highest case fatality rate. Severe piglet mortality makes PEDV infection a major factor in the loss of China's pig industry. The life cycle of a coronavirus begins by invading living cells. After the adsorption is completed, the virus needs to enter the cytoplasm of the host cell in the next step. The virus can in...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K9/52A61K47/36A61P31/14
CPCA61K39/12A61K9/5036A61K2039/5252A61K2039/542A61K2039/552C12N2770/20034
Inventor 姜新鹏高利刘娣
Owner 姜新鹏
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