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Method of degrading surface-source petroleum hydrocarbon in water body through microorganisms

A microbial degradation and biodegradation technology is applied in the field of using microorganisms to degrade non-point source petroleum hydrocarbons in water bodies, which can solve the problems of secondary environmental pollution, poor treatment effect and high cost, and achieves increased contact area, good application prospects and low cost. Effect

Inactive Publication Date: 2018-06-01
LOGISTICAL ENGINEERING UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the above-mentioned deficiencies existing in the prior art, the object of the present invention is to provide a kind of method that utilizes microorganism to degrade water surface source petroleum hydrocarbons, solve existing method for processing river water surface source petroleum hydrocarbon pollution to have high cost, secondary environmental pollution and Dealing with poor performance and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) In waters heavily polluted by petroleum hydrocarbons, 5 g of water samples were collected, and the samples were added to LB liquid medium, and shaken in a constant temperature shaking incubator at 30 °C and a speed of 200 r / min for 12 h.

[0026] The preparation method of LB liquid medium: Weigh 5g of tryptone, 2.5g of yeast extract, and 5g of NaCl respectively, then use deionized water to make up to 300mL, adjust the pH to 7.0 with 5mol / L NaOH solution, and use 121℃ Steam sterilize for 20 min.

[0027] 2) Spread the bacterial liquid obtained in step 1) on an LB plate, and separate and purify a single bacterial strain. Through the identification of 16sRNA, 5 kinds of rapid petroleum hydrocarbon degrading bacteria were obtained: Pseudomonas, Paleobacterium, Bordetella, Gordonia and Pseudomonas aeruginosa, bacteria preservation, standby.

[0028] 3) Inoculate the screened and enriched Pseudomonas, Pseudomonas pallidum, Bordetella, Gordonia and Pseudomonas aeruginosa i...

Embodiment 2

[0035] 1) In waters heavily polluted by petroleum hydrocarbons, 5 g of water samples were collected, and the samples were added to LB liquid medium, and shaken in a constant temperature shaking incubator at 30 °C and a speed of 200 r / min for 12 h.

[0036] 2) Spread the bacterial liquid obtained in step 1) on an LB plate, and separate and purify a single bacterial strain. Through the identification of 16sRNA, 4 kinds of rapid petroleum hydrocarbon degrading bacteria were obtained: Paleobacterium pallidus, Bordetella, Gordonella and Pseudomonas aeruginosa, bacteria preservation, standby.

[0037] 3) Inoculate the screened and enriched Pseudomonas pallidum, Bordetella, Gordonia and Pseudomonas aeruginosa into LB liquid medium respectively, and then place them at a constant temperature of 30°C and 200r / min Shaking culture in a shaking incubator for 36 hours;

[0038] 4) Centrifuge the bacteria cultured in step 3), remove the supernatant, collect the bacteria, and then resuspend ...

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Abstract

The invention discloses a method of degrading surface-source petroleum hydrocarbon in a water body through microorganisms. In the method, the surface-source petroleum hydrocarbon in the water body ishigh-effectively degraded by synergistically combining a biodegradation accelerant N-acyl glutamate, a special microorganism flora and a microbial nutritional composition, thus achieving bio-restoration. Removal rate of the surface-source petroleum hydrocarbon on the polluted water body can reach 100% after about 10 days. The method greatly improves biodegradation efficiency, wherein the microbialdegradation products of the petroleum hydrocarbon are CO2 and H2O, so that the method is free of secondary pollution to environment and is an ideal environment-friendly petroleum hydrocarbon removalmethod. The method has simple operations and employs low-cost and easy-to-obtain raw materials, is free of influence by natural environment, is low in cost, and has great application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioremediation, and in particular relates to a method for degrading non-point source petroleum hydrocarbons in water by microorganisms. Background technique [0002] With the continuous development of society, human's demand for energy is increasing day by day. Petroleum is a valuable energy source and chemical raw material, and its popularization and use has brought great convenience to human production and life. But at the same time, due to improper use and increasingly frequent crude oil spill accidents, while oil, the double-edged sword, is playing a positive role, its negative impact cannot be ignored. Studies have shown that petroleum hydrocarbons have polluted groundwater for as long as 100 years, and 0.1 μg / g of petroleum hydrocarbons can reduce the lifespan of small shrimps in the sea by 20%. In recent years, more than 10 million tons / year of petroleum hydrocarbon-based chemicals have entered t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/38C12R1/385C12R1/01
CPCC02F3/34C02F3/344C02F2101/32C02F2103/007C02F2305/06C12N1/20
Inventor 方建华吴江张楠谷科城刘坪
Owner LOGISTICAL ENGINEERING UNIVERSITY OF PLA
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