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Staphylococcus albus powder and application thereof

A technology of staphylococcus albus and staphylococcus, applied in the field of microorganisms, can solve the problem of high fermentation cost of staphylococcus albus, achieve the effect of increasing industrial added value, high industrial added value, and avoiding damage

Inactive Publication Date: 2018-05-18
仁和堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the defect of high fermentation cost of Staphylococcus albus in the prior art, and provide Staphylococcus albus powder prepared by a new fermentation process and its application

Method used

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  • Staphylococcus albus powder and application thereof
  • Staphylococcus albus powder and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A kind of Staphylococcus albus powder, which is prepared by inoculating the seed liquid of Staphylococcus albus into a wheat bran medium for fermentation;

[0030] Specifically, the staphylococcus albus powder is prepared according to the following process:

[0031] 1) Take Staphylococcus albus solution (5×10 7 CFU / mL), according to the inoculum amount of 3%, inoculated into the seed medium, 30°C, 100rpm shaker culture for 18h, to obtain the seed liquid; the components of the seed medium are: glucose 12g / L, agar 7g / L , peptone 3g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, MgSO 4 0.1g / L, FeSO 4 0.1g / L, pH7.5;

[0032] 2) Inoculate the seed liquid with 5% inoculum amount in a fermenter with 50L wheat bran medium for fermentation culture, culture temperature is 30°C, ventilation rate keeps dissolved oxygen level at 20%, and sugar content is controlled by adding glucose The amount is not less than 0.2wt%, and it is fermented and cultivated for 54 hours to obtain Sta...

Embodiment 2

[0039] A kind of Staphylococcus albus powder, which is prepared by inoculating the seed liquid of Staphylococcus albus into a wheat bran medium for fermentation;

[0040] Specifically, the staphylococcus albus powder is prepared according to the following process:

[0041] 1) Take Staphylococcus albus solution (7×10 7 CFU / mL), inoculated into the seed medium according to the inoculation amount of 2%, and cultured on a shaker at 32°C and 100rpm for 24 hours to obtain the seed liquid; the components of the seed medium were: glucose 12g / L, agar 7g / L , peptone 3g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, MgSO 4 0.1g / L, FeSO 4 0.1g / L, pH7.5;

[0042]2) Inoculate the seed liquid with 3% inoculum amount in a fermenter with 50L wheat bran medium for fermentation and culture, the culture temperature is 32°C, the ventilation rate keeps the dissolved oxygen level at 20%, and the sugar content is controlled by adding glucose The amount is not less than 0.2wt%, fermented and culti...

Embodiment 3

[0049] Nitrosalicylic acid colorimetric method detects the content of reducing sugar in component A:

[0050] Taking Example 1 as an example, the influence of temperature and the amount of enzyme added on the reducing sugar content was detected, and the temperature gradient was set as: 40°C, 50°C, 60°C, 70°C, 80°C; the enzyme amount gradient was set as: 0.2U / g, 0.4U / g, 0.6U / g, 0.8U / g, 1.0U / g. Such as figure 1 As shown, when the temperature is lower than 70°C, as the temperature rises, the content of reducing sugar increases, and at about 70°C, the content of reducing sugar obtained by hydrolysis is the highest, indicating that the activity of mesophilic amylase at 70°C can be Reaching a relatively high level; while the temperature of 80 ℃ reducing sugar content decreased sharply, this may be because the high temperature reduces the activity of amylase at medium temperature, and it may also be accompanied by the consumption of a large amount of reducing sugar. Such as figu...

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Abstract

The invention belongs to the technical field of microbes and discloses staphylococcus albus powder. The staphylococcus albus powder is prepared by inoculating a wheat bran nutrient medium with a staphylococcus albus seed solution and performing fermentation and culture. The staphylococcus albus powder disclosed by the invention is prepared by adopting a special fermentation technique, has the advantage of low cost and can be applied to preparation of cough medicines.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to staphylococcus albus powder and its application. Background technique [0002] Staphylococcus albus powder is a non-pathogenic oral bacterial preparation, which is made of inactivated dry bacteria obtained from liquid culture of non-pathogenic Staphylococcus albus. Experiments show that it has antitussive strength similar to that of codeine, acts directly on the medulla cough center, and has an obvious antitussive effect on acute cough. The larger the dose, the stronger the antitussive effect, and it can regulate the autonomic nervous system function, which can make The amount of sputum was significantly reduced. The preparation of Staphylococcus albus powder by fermentation is the only way for pharmaceutical companies to produce Staphylococcus albus powder, but the cost of the medium restricts the development of the company. At present, many companies use glucose-peptone mediu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61K9/19A61P11/14C12R1/44
CPCA61K9/19A61K35/74C12N1/20
Inventor 林祥宇姚其胜聂昌盛
Owner 仁和堂药业有限公司
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